AB168363

Anti-Nucleolin (phospho T76) antibody [EPR8080-2]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal Nucleolin phospho T76 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

Nucleolin, Protein C23, NCL

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional cell carcinoma tissue labeling Nucleolin with ab168363 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Nucleolin with ab168363 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

ab168363 showing +ve staining in Human ovarian carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

ab168363 showing +ve staining in Human endometrial carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

ab168363 showing +ve staining in Human breast carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

ab168363 showing +ve staining in Human kidney carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

IP

Unknown

Immunoprecipitation - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

Western blot analysis on immunoprecipitation pellet from 293 cell lysate using anti Phospho Nucleolin (pT76) ab158363 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

Predicted band size: 76 kDa

false

Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

WB

Unknown

Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (AB168363)

All lanes:

Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363) at 1/1000 dilution

Lane 1:

293T cell lysates, untreated at 10 µg

Lane 2:

293T cell lysates, treated with Lambda Phosphatase at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 76 kDa

false

Carrier free

Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Nucleolin (phospho T76) antibody [EPR8080-2]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Nucleolin (phospho T76) antibody [EPR8080-2]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Nucleolin (phospho T76) antibody [EPR8080-2]
660 APC

APC Anti-Nucleolin (phospho T76) antibody [EPR8080-2]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Nucleolin (phospho T76) antibody [EPR8080-2]
578 PE

PE Anti-Nucleolin (phospho T76) antibody [EPR8080-2]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Nucleolin (phospho T76) antibody [EPR8080-2]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8080-2

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P, IP, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleolin also known as C23 is a multifunctional protein mainly found in the nucleolus with a molecular weight of approximately 100 kDa. It plays a mechanical role in ribosomal RNA (rRNA) synthesis and ribosome biogenesis. Nucleolin is widely expressed in proliferating cells including HeLa culture and shows higher expression in cancer cells compared to normal cells. The nucleolin protein is involved in various nucleolar processes like the modulation of chromatin structure and nucleocytoplasmic transport.

Biological function summary

The nucleolin molecular weight protein contributes to regulating cellular processes such as chromatin remodeling rRNA transcription and mRNA stability. Nucleolin often forms complexes with various other proteins and nucleic acids enhancing its functionality within the cell nucleus. Its participation in processes like DNA replication and cell proliferation shows the central role nucleolin plays at the cellular level. It influences cellular responses by binding directly to specific RNA or DNA sequences.

Pathways

The nucleolin protein engages in major cellular pathways such as the RNA processing and p53 signaling pathways. Through the RNA processing pathway it interacts with proteins like fibrillarin to assist in rRNA maturation. Moreover within the p53 signaling pathway nucleolin regulates the activity of the tumor suppressor protein p53 impacting cell cycle progression and apoptosis. These interactions highlight nucleolin's role in maintaining cellular homeostasis and response to stress.

The nucleolin protein often shows altered expression in cancers and cardiovascular diseases. In cancer nucleolin's high expression levels correlate with tumor progression and metastasis making it a potential target for cancer therapies. In cardiovascular diseases nucleolin interacts with proteins like VEGF to influence angiogenesis which affects disease progression. Understanding nucleolin's involvement in these diseases provides insight into potential therapeutic targets and interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

See full target information NCL phospho T76

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2579 PubMed40089461

2025

High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells.

Applications

Unspecified application

Species

Unspecified reactive species

Camilla Rega,Ifigenia Tsitsa,Theodoros I Roumeliotis,Izabella Krystkowiak,Maria Portillo,Lu Yu,Julia Vorhauser,Jonathon Pines,Jörg Mansfeld,Jyoti Choudhary,Norman E Davey

Frontiers in cell and developmental biology 9:728821 PubMed34733844

2021

Controls Angiogenesis by Targeting VE-PTP Through Interaction With Nucleolin.

Applications

Unspecified application

Species

Unspecified reactive species

Honglin Luo,Yongde Zhang,Yanfei Deng,Lequn Li,Zhaoan Sheng,Yanling Yu,Yong Lin,Xiaohan Chen,Pengfei Feng

Oncology reports 41:590-598 PubMed30320343

2018

Phosphorylation of nucleolin is indispensable to its involvement in the proliferation and migration of non-small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Huang,Yanyang Wu,Hong Tan,Tianyao Guo,Ke Zhang,Daiqiang Li,Zhongyi Tong

Clinical proteomics 13:26 PubMed27708557

2016

Plasma degradome affected by variable storage of human blood.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Kaisar,Leon F A van Dullemen,Marie-Laëtitia Thézénas,M Zeeshan Akhtar,Honglei Huang,Sandrine Rendel,Philip D Charles,Roman Fischer,Rutger J Ploeg,Benedikt M Kessler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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