Anti-Nucleophosmin antibody [FC82291]

• WB

Lab

Western blot - Anti-Nucleophosmin antibody [FC82291] (AB10530)

False colour image of Western blot : Anti-BRD2 antibody [EPR7642] - ChIP Grade staining at 1/1000 dilution, shown in green; Mouse Anti-Nucleophosmin antibody [FC82291] (ab10530) loading control staining at 2 ug/mL imaged in ECL. In Western blot, ab139690 was shown to bind specifically to BRD2. A band was observed at 110 kDa in wild-type A549 cell lysates with no signal observed at this size in BRD2 knockout cell line. To generate this image, wild-type and BRD2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween®20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 20 seconds exposure time. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and HRP conjugated Goat anti-Mouse (H+L) at 1/20000 dilution.

All lanes:

Western blot - Anti-BRD2 antibody [EPR7642] - ChIP Grade (<a href='/en-us/products/primary-antibodies/brd2-antibody-epr7642-chip-grade-ab139690'>ab139690</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 ab288558 cytoplasm lysate at 20 µg

Lane 2:

Wild-type A549 ab288558 nuclear lysate at 20 µg

Lane 3:

BRD2 knockout A549 cytoplasm lysate at 20 µg

Lane 4:

BRD2 knockout A549 nuclear lysate at 20 µg

Lane 5:

Wild-type HEK-293T ab255553 cell lysate at 20 µg

Lane 6:

BRD2 knockout HEK-293T cell lysate at 20 µg

Observed band size: 110 kDa

false

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [FC82291] (AB10530)

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells staining Nucleophosmin using ab10530 (dilution 1/500). Cells were fixed and permeabilized with methanol followed by methanol : acetone. Developed using Goat Anti-Mouse IgG, Cy3™ conjugate and counterstained with DAPI (blue) to stain nuclei.

• ICC/IF

AbReview23076****

Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [FC82291] (AB10530)

ab10530 staining Nucleophosmin in immortalized Human trabecular meshwork cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 3% BSA for 45 minutes at room temperature. The primary antibody was diluted 1/1000 in PBS/0.3% BSA and incubated with the sample for 2 hours. The secondary antibody was Cy3^®-conjugated Donkey anti-Mouse polyclonal, diluted 1/1000.

This image is courtesy of an Anonymous Abreview.

• WB

Supplier Data

Western blot - Anti-Nucleophosmin antibody [FC82291] (AB10530)

Cell line lysate separated on SDS-PAGE, probed with ab10530 (0.5 μg/mL)

All lanes:

Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530) at 0.5 µg/mL

Lane 1:

HeLa cell lysate

Lane 2:

JURKAT cell lysate

Lane 3:

COS7 cell lysate

Lane 4:

NIH-3T3 cell lysate

Lane 5:

RAT2 cell lysate

Lane 6:

CHO cell lysate

Lane 7:

MDBK cell lysate

Lane 8:

MDCK cell lysate

Secondary

All lanes:

Goat Anti-Mouse IgG-Peroxidase

Predicted band size: 32 kDa

false

• WB

AbReview8715****

Western blot - Anti-Nucleophosmin antibody [FC82291] (AB10530)

Western blot image of ab10530 staining whole cell lysate of U2OS cells. The gel was blocked with 5% milk for 1 hour at 21°C. The primary antibody was diluted to 2 μg/ml and incubated for 12 hours at 4°C. A HRP conjugated goat anti-mouse antibody was used as the secondary.

All lanes:

Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530)

Predicted band size: 32 kDa

Observed band size: 38 kDa

false

This image is courtesy of an anonymous Abreview

• WB

Supplier Data

Western blot - Anti-Nucleophosmin antibody [FC82291] (AB10530)

All lanes:

Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530) at 0.2 µg/mL

All lanes:

3T3 cell lysate

Secondary

All lanes:

Anti-Mouse IgG (Fab)

Predicted band size: 32 kDa

false