Anti-Nucleophosmin antibody [FC82291]

6 product images

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  Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530)

  False colour image of Western blot: Anti-BRD2 antibody [EPR7642] - ChIP Grade staining at 1/1000 dilution, shown in green; Mouse Anti-Nucleophosmin antibody [FC82291] (ab10530) loading control staining at 2 ug/mL imaged in ECL. In Western blot, Anti-BRD2 antibody [EPR7642] - ChIP Grade ab139690 was shown to bind specifically to BRD2. A band was observed at 110 kDa in wild-type A549 cell lysates with no signal observed at this size in BRD2 knockout cell line. To generate this image, wild-type and BRD2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween®20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent Anti-PKC delta (phospho S299) antibody [EPNCI119] ab133456) and imaged with 20 seconds exposure time. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and HRP conjugated Goat anti-Mouse (H+L) at 1/20000 dilution.

  All lanes: Western blot - Anti-BRD2 antibody [EPR7642] - ChIP Grade (Anti-BRD2 antibody [EPR7642] - ChIP Grade ab139690) at 1/1000 dilution

  Lane 1: Wild-type A549 ab288558 cytoplasm lysate at 20 µg

  Lane 2: Wild-type A549 ab288558 nuclear lysate at 20 µg

  Lane 3: BRD2 knockout A549 cytoplasm lysate at 20 µg

  Lane 4: BRD2 knockout A549 nuclear lysate at 20 µg

  Lane 5: Wild-type HEK-293T ab255553 cell lysate at 20 µg

  Lane 6: BRD2 knockout HEK-293T cell lysate at 20 µg

  Performed under reducing conditions.

  Observed band size: 110 kDa

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  This image is courtesy of an anonymous customer review

  Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530)

  Western blot image of ab10530 staining whole cell lysate of U2OS cells. The gel was blocked with 5% milk for 1 hour at 21°C. The primary antibody was diluted to 2 μg/ml and incubated for 12 hours at 4°C. A HRP conjugated goat anti-mouse antibody was used as the secondary.

  All lanes: Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530)

  Predicted band size: 32 kDa

  Observed band size: 38 kDa

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  Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530)

  All lanes: Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530) at 0.2 µg/mL

  All lanes: 3T3 cell lysate

  Secondary

  All lanes: Anti-Mouse IgG (Fab)

  Predicted band size: 32 kDa

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  Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530)

  Cell line lysate separated on SDS-PAGE, probed with ab10530 (0.5 μg/mL)

  All lanes: Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530) at 0.5 µg/mL

  Lane 1: HeLa cell lysate

  Lane 2: JURKAT cell lysate

  Lane 3: COS7 cell lysate

  Lane 4: NIH-3T3 cell lysate

  Lane 5: RAT2 cell lysate

  Lane 6: CHO cell lysate

  Lane 7: MDBK cell lysate

  Lane 8: MDCK cell lysate

  Secondary

  All lanes: Goat Anti-Mouse IgG-Peroxidase

  Predicted band size: 32 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [FC82291] (ab10530)

  Immunocytochemistry/ Immunofluorescence analysis of HeLa cells staining Nucleophosmin using ab10530 (dilution 1/500). Cells were fixed and permeabilized with methanol followed by methanol:acetone. Developed using Goat Anti-Mouse IgG, Cy3™ conjugate and counterstained with DAPI (blue) to stain nuclei.

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  This image is courtesy of an Anonymous customer review.

  Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [FC82291] (ab10530)

  ab10530 staining Nucleophosmin in immortalized Human trabecular meshwork cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 3% BSA for 45 minutes at room temperature. The primary antibody was diluted 1/1000 in PBS/0.3% BSA and incubated with the sample for 2 hours. The secondary antibody was Cy3^®-conjugated Donkey anti-Mouse polyclonal, diluted 1/1000.