AB256124

Anti-Nucleophosmin antibody [NA24] (BSA and Azide free)

Recombinant
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(5 Publications)

Mouse Recombinant Monoclonal Nucleophosmin antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

NPM, NPM1, Nucleophosmin, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on human liver. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Nucleophosmin with ab238029 at 1/100 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cells. ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker was used to counterstain tubulin at 1/200 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary at 1/2000 diluton (Red). The Nuclear counterstain was DAPI (Blue).

The negative controls are as follows :
-ve control 1 : ab238029 at 1/100 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary at 1/1000 dilution.
-ve control 2 : ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Nucleophosmin with ab238029 at 1/500 dilution (Red) compared with a Mouse monoclonal IgG isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-mouse IgG (Alexa Fluor^® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) . Nuclear staining on human pancreas. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on mouse stomach is observed. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) . Nuclear staining on mouse liver is observed. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 3T3-L1 (Mouse embryonic fibroblast) cells labelling Nucleophosmin with ab238029 at 1/500 dilution (Red) compared with a Mouse monoclonal IgG isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-mouse IgG (Alexa Fluor^® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat liver is observed. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat colon is observed. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

Unconjugated

Anti-Nucleophosmin antibody [NA24] (N-terminal)

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NA24

Isotype

IgG1

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "ProteinArray-species-checked": "notRecommended", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "Incubate with primary antibody for 15 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ProteinArray-species-checked": "notRecommended", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ProteinArray-species-checked": "notRecommended", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

ab256124 is the carrier-free version of ab238029.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleophosmin also known as NPM1 or B23 is a multifunctional nucleolar phosphoprotein with a molecular weight of approximately 37 kDa. It is highly expressed in the nucleolus of many human cells and is involved in various cellular processes. NPM1 aids ribosome biogenesis intracellular transport of ribosomal components and regulation of the centrosome. Its nucleocytoplasmic shuttling ability is due to its N-terminal domain which plays an important role in binding to nucleic acids and other proteins. Apart from these functions NPM1 acts as a histone chaperone and is important for maintaining genomic integrity.

Biological function summary

One of the central roles of NPM1 is its involvement in the regulation of cell growth and proliferation. NPM1 forms a complex with other nucleolar proteins affecting ribosome subunit assembly and transport. This protein also participates in the control of the ARF/p53 pathway by sequestering p53-regulating proteins which are essential for cell cycle control and apoptosis. Its interactions with proteins such as nucleolin and nucleophosmin-like proteins allow NPM1 to perform its functions in organizing the nucleolus and regulating responses to stress signals.

Pathways

The NPM1 protein is integrally involved in key cellular pathways like the regulation of the cell cycle and apoptosis. It directly interacts with pathways such as the ribosome biogenesis pathway and the p53 pathway. NPM1 has connections with proteins like ARF and MDM2 which play roles in retinoblastoma protein (pRb) regulation and in the control of tumor-suppressor activities. These pathways under NPM1’s influence are critical for maintaining cellular homeostasis and ensuring proper cell cycle checkpoints.

NPM1 mutations are commonly observed in acute myeloid leukemia (AML) where they affect the normal function of the protein leading to leukemogenesis. NPM1 is also linked to tumorigenesis through its impact on the p53 tumor suppressor pathway. Specifically alterations in NPM1 can disrupt the balance of other related proteins like ALK and p53 influencing the onset and progression of leukemia. Understanding the roles of NPM1 in these contexts is essential for developing targeted therapies to treat AML and other related disorders effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication : phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed : 22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed : 25956029). May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1 (By similarity).

See full target information NPM1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Endocrinology 158:503-515 PubMed28001444

2016

Vitamin D Receptor Expression in Plasmablastic Lymphoma and Myeloma Cells Confers Susceptibility to Vitamin D.

Applications

Unspecified application

Species

Unspecified reactive species

Duncan M Gascoyne,Linden Lyne,Hayley Spearman,Francesca M Buffa,Elizabeth J Soilleux,Alison H Banham

Molecular and cellular biology 34:4474-84 PubMed25288641

2014

mTOR signaling regulates nucleolar targeting of the SUMO-specific isopeptidase SENP3.

Applications

Unspecified application

Species

Unspecified reactive species

Nithya Raman,Arnab Nayak,Stefan Muller

The EMBO journal 30:1067-78 PubMed21326211

2011

The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth Finkbeiner,Markus Haindl,Stefan Muller

Proteomics 10:2812-21 PubMed20533335

2010

Proteomic analysis of low- to high-grade astrocytomas reveals an alteration of the expression level of raf kinase inhibitor protein and nucleophosmin.

Applications

Unspecified application

Species

Unspecified reactive species

Marcela Gimenez,Vanessa Cristina de Oliveira Souza,Clarice Izumi,Manuela R Barbieri,Roger Chammas,Sueli Mieko Oba-Shinjo,Miyuki Uno,Suely Kazue Nagahashi Marie,José Cesar Rosa

Blood 93:632-42 PubMed9885226

1999

Detection of normal and chimeric nucleophosmin in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

J L Cordell,K A Pulford,B Bigerna,G Roncador,A Banham,E Colombo,P G Pelicci,D Y Mason,B Falini

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nucleophosmin antibody [NA24] (BSA and Azide free)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (BSA and Azide free) (AB256124)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise