Anti-Nucleophosmin antibody [NA24] (N-terminal)

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (N-terminal) (AB238029)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on human liver. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (N-terminal) (AB238029)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) . Nuclear staining on human pancreas. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [NA24] (N-terminal) (AB238029)

ab238029 staining Nucleophosmin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab238029 at 1μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (N-terminal) (AB238029)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) . Nuclear staining on mouse liver is observed. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (N-terminal) (AB238029)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on mouse stomach is observed. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (N-terminal) (AB238029)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat liver is observed. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (N-terminal) (AB238029)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat colon is observed. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

• WB

Lab

Western blot - Anti-Nucleophosmin antibody [NA24] (N-terminal) (AB238029)

Lanes 1 - 7 : Merged signal (red and green). Green - ab238029 observed at 36 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa. ab238029 was shown to react with Nucleophosmin in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab238029 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Nucleophosmin antibody [NA24] (N-terminal) (ab238029) at 1 µg/mL

Lane 1:

Daudi whole cell lysate at 20 µg

Lane 2:

Jurkat whole cell lysate at 20 µg

Lane 3:

Caco-2 whole cell lysate at 20 µg

Lane 4:

Sample: HeLa whole cell lysate at 20 µg

Lane 5:

NIH/3T3 whole cell lysate at 20 µg

Lane 6:

MEF whole cell lysate at 20 µg

Lane 7:

PC-12 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/20000 dilution

Predicted band size: 32 kDa

Observed band size: 36 kDa

false

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] (N-terminal) (AB238029)

Staining performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab238029, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX