Anti-Nucleophosmin antibody [SP236] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (AB245744)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling Nucleophosmin with ab183340 at 1/100 dilution (2.51 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340)

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (AB245744)

Intracellular Flow Cytometry analysis of 3T3-L1 (Mouse embryonic fibroblast) labeling Nucleophosmin with purified ab183340 at 1/200 dilution (1.255μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (AB245744)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling Nucleophosmin with ab183340 at 1/100 dilution (2.51 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (AB245744)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Nucleophosmin with ab183340 at a 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (ab183340)

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (AB245744)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Nucleophosmin with purified ab183340 at 1/200 dilution (1.255μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (AB245744)

Immunocytochemistry/ Immunofluorescence analysis of 3T3-L1 (mouse embryonic fibroblast) cells labeling Nucleophosmin with purified ab183340 at 1/100 (2.5μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (AB245744)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling Nucleophosmin with ab183340 at 1/100 dilution (2.51 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340)

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (AB245744)

Immunocytochemistry/ Immunofluorescence analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labeling Nucleophosmin with purified ab183340 at 1/100(2.5μg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (AB245744)

Intracellular flow cytometric analysis of Jurkat cells, labeling Nucleophosmin with ab183340diluted 1/400 (green), compared with a negative control of anti-rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183340).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (AB245744)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Nucleophosmin with purified ab183340 at 1/100 (2.5μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340).