AB208015

Anti-Nucleophosmin (citrulline R196) antibody [EPR20172]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal Nucleophosmin citrulline R196 antibody. Suitable for IP, Dot, WB and reacts with Human, Rat, Mouse, Synthetic peptide samples. Cited in 2 publications.

View Alternative Names

Nucleophosmin, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, Npm1

4 Images

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Immunoprecipitation - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (AB208015)

Nucleophosmin (citrulline R196) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 expression vector for 24h then treated with 10 mM CaCl₂ and 10 μM ionomycin for 2h, whole cell lysate with ab208015 at 1/30 dilution. Western blot was perfromed from the immunoprecipitate using ab208015 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution.

Lane 1 : HEK-293T transfected with GFP-tagged PADI4 expression vector for 24h then treated with 10 mM CaCl₂ and 10 μM ionomycin for 2h, whole cell lysate 10μg (Input).

Lane 2 : ab208015 IP in HEK-293T transfected with GFP-tagged PADI4 expression vector for 24h then treated with 10 mM CaCl₂ and 10 μM ionomycin for 2h, whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab208015 in HEK-293T transfected with GFP-tagged PADI4 expression vector for 24h then treated with 10mM CaCl₂ and 10 μM ionomycin for 2h, whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

Exposure time : lesss than 1 second.

All lanes:

Immunoprecipitation - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (ab208015)

Predicted band size: 32 kDa

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Supplier Data

Western blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (AB208015)

Blocking/Dilution buffer : 5% NFDM/TBST.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

All lanes:

Western blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (ab208015) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate at 10 µg

Lane 2:

NIH/3T3 transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate at 10 µg

Predicted band size: 32 kDa

Observed band size: 37 kDa

true

Exposure time: 15s

Supplier Data

Western blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (AB208015)

Exposure time : Lane 1 and 2 : 5 seconds; Lane 3 and 4 : 3 minutes.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (ab208015) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with GFP-tagged PADI2 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate at 20 µg

Lane 3:

C6 (rat glial tumor cell line) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate at 20 µg

Lane 4:

C6 transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate at 20 µg

Predicted band size: 32 kDa

Observed band size: 37 kDa

true

Supplier Data

Dot Blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (AB208015)

Dot blot analysis of Nucleophosmin (citruline R196) labeled with ab208015 at 1/1000 dilution.

Lane 1 : Nucleophosmin (citrulline R196) peptide.

Lane 2 : Nucleophosmin non-citrulline peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Carrier free

Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20172

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleophosmin also known as NPM1 or B23 is a multifunctional nucleolar phosphoprotein with a molecular weight of approximately 37 kDa. It is highly expressed in the nucleolus of many human cells and is involved in various cellular processes. NPM1 aids ribosome biogenesis intracellular transport of ribosomal components and regulation of the centrosome. Its nucleocytoplasmic shuttling ability is due to its N-terminal domain which plays an important role in binding to nucleic acids and other proteins. Apart from these functions NPM1 acts as a histone chaperone and is important for maintaining genomic integrity.

Biological function summary

One of the central roles of NPM1 is its involvement in the regulation of cell growth and proliferation. NPM1 forms a complex with other nucleolar proteins affecting ribosome subunit assembly and transport. This protein also participates in the control of the ARF/p53 pathway by sequestering p53-regulating proteins which are essential for cell cycle control and apoptosis. Its interactions with proteins such as nucleolin and nucleophosmin-like proteins allow NPM1 to perform its functions in organizing the nucleolus and regulating responses to stress signals.

Pathways

The NPM1 protein is integrally involved in key cellular pathways like the regulation of the cell cycle and apoptosis. It directly interacts with pathways such as the ribosome biogenesis pathway and the p53 pathway. NPM1 has connections with proteins like ARF and MDM2 which play roles in retinoblastoma protein (pRb) regulation and in the control of tumor-suppressor activities. These pathways under NPM1’s influence are critical for maintaining cellular homeostasis and ensuring proper cell cycle checkpoints.

NPM1 mutations are commonly observed in acute myeloid leukemia (AML) where they affect the normal function of the protein leading to leukemogenesis. NPM1 is also linked to tumorigenesis through its impact on the p53 tumor suppressor pathway. Specifically alterations in NPM1 can disrupt the balance of other related proteins like ALK and p53 influencing the onset and progression of leukemia. Understanding the roles of NPM1 in these contexts is essential for developing targeted therapies to treat AML and other related disorders effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication : phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade. In complex with MYC enhances the transcription of MYC target genes. May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1 (PubMed : 20513429).

See full target information Npm1 citrulline R196

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadl2238 PubMed38394209

2024

Identification of the metaphyseal skeletal stem cell building trabecular bone.

Applications

Unspecified application

Species

Unspecified reactive species

Guan Yang,Qi He,Xiaoxiao Guo,Rong-Yu Li,Jingting Lin,Yiming Lang,Wanyu Tao,Wenjia Liu,Huisang Lin,Shilai Xing,Yini Qi,Zhongliang Xie,Jing-Dong J Han,Bin Zhou,Yan Teng,Xiao Yang

Clinical and translational medicine 12:e944 PubMed35735113

2022

Lipid metabolism-related lncRNA SLC25A21-AS1 promotes the progression of oesophageal squamous cell carcinoma by regulating the NPM1/c-Myc axis and SLC25A21 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Liu,Chunxiang Li,Lingling Fang,Liyu Wang,Hengchang Liu,He Tian,Yujia Zheng,Tao Fan,Jie He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nucleophosmin (citrulline R196) antibody [EPR20172]

Immunoprecipitation - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (AB208015)

Western blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (AB208015)

Western blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (AB208015)

Dot Blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (AB208015)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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