AB109546

Anti-Nucleophosmin (phospho S125) antibody [EPR1856]

Name: Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546) | Abcam
Brand: Abcam Limited
SKU: AB109546
Price: 380 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal Nucleophosmin phospho S125 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

NPM, NPM1, Nucleophosmin, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (AB109546)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (AB109546)

ab109546 at a 1/100 dilution staining Nucleophosmin in formalin-fixed paraffin-embedded Human kidney tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (AB109546)

ab109546 at a 1/100 dilution staining Nucleophosmin in formailn-fixed paraffin-embedded Human breast tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (AB109546)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (AB109546)

Overlay histogram showing HeLa cells stained with ab109546 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109546 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Unknown

Western blot - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (AB109546)

All lanes:

Western blot - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HeLa cell lysate treated with Alkaline Phosphatase at 10 µg

Predicted band size: 32 kDa

false

Carrier free

Anti-Nucleophosmin (phospho S125) antibody [EPR1856] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1856

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleophosmin also known as NPM1 or B23 is a multifunctional nucleolar phosphoprotein with a molecular weight of approximately 37 kDa. It is highly expressed in the nucleolus of many human cells and is involved in various cellular processes. NPM1 aids ribosome biogenesis intracellular transport of ribosomal components and regulation of the centrosome. Its nucleocytoplasmic shuttling ability is due to its N-terminal domain which plays an important role in binding to nucleic acids and other proteins. Apart from these functions NPM1 acts as a histone chaperone and is important for maintaining genomic integrity.

Biological function summary

One of the central roles of NPM1 is its involvement in the regulation of cell growth and proliferation. NPM1 forms a complex with other nucleolar proteins affecting ribosome subunit assembly and transport. This protein also participates in the control of the ARF/p53 pathway by sequestering p53-regulating proteins which are essential for cell cycle control and apoptosis. Its interactions with proteins such as nucleolin and nucleophosmin-like proteins allow NPM1 to perform its functions in organizing the nucleolus and regulating responses to stress signals.

Pathways

The NPM1 protein is integrally involved in key cellular pathways like the regulation of the cell cycle and apoptosis. It directly interacts with pathways such as the ribosome biogenesis pathway and the p53 pathway. NPM1 has connections with proteins like ARF and MDM2 which play roles in retinoblastoma protein (pRb) regulation and in the control of tumor-suppressor activities. These pathways under NPM1’s influence are critical for maintaining cellular homeostasis and ensuring proper cell cycle checkpoints.

NPM1 mutations are commonly observed in acute myeloid leukemia (AML) where they affect the normal function of the protein leading to leukemogenesis. NPM1 is also linked to tumorigenesis through its impact on the p53 tumor suppressor pathway. Specifically alterations in NPM1 can disrupt the balance of other related proteins like ALK and p53 influencing the onset and progression of leukemia. Understanding the roles of NPM1 in these contexts is essential for developing targeted therapies to treat AML and other related disorders effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication : phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed : 22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed : 25956029). May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1 (By similarity).

See full target information NPM1 pS125

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communications 681:194-199 PubMed37783117

2023

Phosphoproteome analysis of the crosstalk between sumoylation and phosphorylation in mouse spermatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Noa Applebaum,Sara Chemel,Shaina Matveev,Sayanto Subrato Pal,Amitabha Sengupta,Benjamin Lucas,Margarita Vigodner

Molecular & cellular proteomics : MCP 22:100488 PubMed36563749

2022

HIV-1 Transcription Inhibitor 1E7-03 Decreases Nucleophosmin Phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Xionghao Lin,Asrar Ahmad,Andrey I Ivanov,Jyothirmai Simhadri,Songping Wang,Namita Kumari,Tatiana Ammosova,Sergei Nekhai

Journal of cellular physiology 233:8826-8838 PubMed29806702

2018

Interaction of CacyBP/SIP with NPM1 and its influence on NPM1 localization and function in oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Rosińska,Anna Filipek

eLife 3:e01641 PubMed24867636

2014

Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery.

Applications

ICC/IF

Species

Human

Daniel G Booth,Masatoshi Takagi,Luis Sanchez-Pulido,Elizabeth Petfalski,Giulia Vargiu,Kumiko Samejima,Naoko Imamoto,Chris P Ponting,David Tollervey,William C Earnshaw,Paola Vagnarelli

Cell cycle (Georgetown, Tex.) 13:1227-36 PubMed24675893

2014

MKK7 and ARF: new players in the DNA damage response scenery.

Applications

Unspecified application

Species

Unspecified reactive species

Athanassios Kotsinas,Panagiota Papanagnou,Panagiotis Galanos,Daniel Schramek,Paul Townsend,Josef M Penninger,Jiri Bartek,Vassilis G Gorgoulis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nucleophosmin (phospho S125) antibody [EPR1856]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (AB109546)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (AB109546)

Flow Cytometry (Intracellular) - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (AB109546)

Western blot - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (AB109546)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

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Shipped at conditions

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Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise