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AB278648

Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1]

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(1 Publication)

Rabbit Recombinant Monoclonal Nucleophosmin phospho S4 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NPM1 phospho S4.

View Alternative Names

NPM, NPM1, Nucleophosmin, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin

3 Images
Flow Cytometry - Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (AB278648)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (AB278648)

Flow cytometric analysis of K562 cells treated with imatinib (red) or treated with pervanadate (green) using ab278648 at 0.01 ug/mL, or concentration-matched Rabbit (G9) mAb IgG Isotope Control for cells treated with imatinib (black) or treated with pervanadate (blue).

Flow Cytometry - Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (AB278648)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (AB278648)

Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or treated with pervanadate (green) or treated with imatinib and blocked with phospho-peptide (black) or treated with pervanadate and blocked with phospho-peptide (gold) or treated with imatinib and blocked by non-phospho-peptide (dark blue) or treated with pervanadate and block with non-phospho-peptide (purple) using ab278648 at 0.01 ug/mL.

Western blot - Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (AB278648)
  • WB

Supplier Data

Western blot - Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (AB278648)

All lanes:

Western blot - Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (ab278648) at 0.1 µg/mL

Lane 1:

Human epithelial cell line from cervix adenocarcinoma (treated with nocodazole) cell extract

Lane 2:

Human epithelial cell line from cervix adenocarcinoma (untreated) cell extract

Predicted band size: 32 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

NPMS4-A1

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human NPM1 phospho S4. The exact immunogen used to generate this antibody is proprietary information.

P06748

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleophosmin also known as NPM1 or B23 is a multifunctional nucleolar phosphoprotein with a molecular weight of approximately 37 kDa. It is highly expressed in the nucleolus of many human cells and is involved in various cellular processes. NPM1 aids ribosome biogenesis intracellular transport of ribosomal components and regulation of the centrosome. Its nucleocytoplasmic shuttling ability is due to its N-terminal domain which plays an important role in binding to nucleic acids and other proteins. Apart from these functions NPM1 acts as a histone chaperone and is important for maintaining genomic integrity.
Biological function summary

One of the central roles of NPM1 is its involvement in the regulation of cell growth and proliferation. NPM1 forms a complex with other nucleolar proteins affecting ribosome subunit assembly and transport. This protein also participates in the control of the ARF/p53 pathway by sequestering p53-regulating proteins which are essential for cell cycle control and apoptosis. Its interactions with proteins such as nucleolin and nucleophosmin-like proteins allow NPM1 to perform its functions in organizing the nucleolus and regulating responses to stress signals.

Pathways

The NPM1 protein is integrally involved in key cellular pathways like the regulation of the cell cycle and apoptosis. It directly interacts with pathways such as the ribosome biogenesis pathway and the p53 pathway. NPM1 has connections with proteins like ARF and MDM2 which play roles in retinoblastoma protein (pRb) regulation and in the control of tumor-suppressor activities. These pathways under NPM1’s influence are critical for maintaining cellular homeostasis and ensuring proper cell cycle checkpoints.

NPM1 mutations are commonly observed in acute myeloid leukemia (AML) where they affect the normal function of the protein leading to leukemogenesis. NPM1 is also linked to tumorigenesis through its impact on the p53 tumor suppressor pathway. Specifically alterations in NPM1 can disrupt the balance of other related proteins like ALK and p53 influencing the onset and progression of leukemia. Understanding the roles of NPM1 in these contexts is essential for developing targeted therapies to treat AML and other related disorders effectively.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication : phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed : 22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed : 25956029). May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1 (By similarity).
See full target information NPM1 phospho S4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental neurology 373:114656 PubMed38114054

2023

Intranasal administration of recombinant prosaposin attenuates neuronal apoptosis through GPR37/PI3K/Akt/ASK1 pathway in MCAO rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yu,Jinlan Li,Nathanael Matei,Wenna Wang,Lihui Tang,Jinwei Pang,Xue Li,Lili Fang,Jiping Tang,John H Zhang,Min Yan
View all publications
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