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Rabbit Recombinant Monoclonal Nucleophosmin phospho T199 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] - BSA and Azide free (AB208698), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] - BSA and Azide free (AB208698), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] - BSA and Azide free (AB208698), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] - BSA and Azide free (AB208698), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Expected
Tested
Tested
Tested

Expected
Expected

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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1 product for Alternative Version

Target data

Function

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029). May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1 (By similarity).

Alternative names

NPM, NPM1, Nucleophosmin, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin

Recommended products

Rabbit Recombinant Monoclonal Nucleophosmin phospho T199 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP1857Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab208698 is the carrier-free version of Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] ab81551.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Nucleophosmin also known as NPM1 or B23 is a multifunctional nucleolar phosphoprotein with a molecular weight of approximately 37 kDa. It is highly expressed in the nucleolus of many human cells and is involved in various cellular processes. NPM1 aids ribosome biogenesis intracellular transport of ribosomal components and regulation of the centrosome. Its nucleocytoplasmic shuttling ability is due to its N-terminal domain which plays an important role in binding to nucleic acids and other proteins. Apart from these functions NPM1 acts as a histone chaperone and is important for maintaining genomic integrity.

Biological function summary

One of the central roles of NPM1 is its involvement in the regulation of cell growth and proliferation. NPM1 forms a complex with other nucleolar proteins affecting ribosome subunit assembly and transport. This protein also participates in the control of the ARF/p53 pathway by sequestering p53-regulating proteins which are essential for cell cycle control and apoptosis. Its interactions with proteins such as nucleolin and nucleophosmin-like proteins allow NPM1 to perform its functions in organizing the nucleolus and regulating responses to stress signals.

Pathways

The NPM1 protein is integrally involved in key cellular pathways like the regulation of the cell cycle and apoptosis. It directly interacts with pathways such as the ribosome biogenesis pathway and the p53 pathway. NPM1 has connections with proteins like ARF and MDM2 which play roles in retinoblastoma protein (pRb) regulation and in the control of tumor-suppressor activities. These pathways under NPM1’s influence are critical for maintaining cellular homeostasis and ensuring proper cell cycle checkpoints.

Associated diseases and disorders

NPM1 mutations are commonly observed in acute myeloid leukemia (AML) where they affect the normal function of the protein leading to leukemogenesis. NPM1 is also linked to tumorigenesis through its impact on the p53 tumor suppressor pathway. Specifically alterations in NPM1 can disrupt the balance of other related proteins like ALK and p53 influencing the onset and progression of leukemia. Understanding the roles of NPM1 in these contexts is essential for developing targeted therapies to treat AML and other related disorders effectively.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] - BSA and Azide free (ab208698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] - BSA and Azide free (ab208698)

    Immunohistochemistry analysis of paraffin-embedded Human lymphoma tissue, using 1/100 Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] ab81551.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] ab81551).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] - BSA and Azide free (ab208698), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] - BSA and Azide free (ab208698)

    Immunofluorescent staing of HeLa cells using 1/100 Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] ab81551.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] ab81551).

  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] - BSA and Azide free (ab208698), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] - BSA and Azide free (ab208698)

    Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] ab81551 staining Nucleophosmin (phospho T199) in human HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with 0.1% Triton x100 in PBS and blocking with 5% serum was performed for 30 minutes at 230C. Samples were incubated with primary antibody (1/150 in PBS) for 1 hour at 23°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/100 as secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] ab81551).

  • Flow Cytometry (Intracellular) - Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] - BSA and Azide free (ab208698), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] - BSA and Azide free (ab208698)

    Overlay histogram showing HeLa cells stained with Anti-Cytokeratin 19 antibody [EPR1579Y] ab76539 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Cytokeratin 19 antibody [EPR1579Y] ab76539, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] ab81551).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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