AB188413

Anti-Nucleoporin p62/NUP62 antibody [2A]

Name: Anti-Nucleoporin p62/NUP62 antibody [2A] (ab188413) | Abcam
Brand: Abcam Limited
SKU: AB188413
Price: 535 USD
Availability: InStock

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(6 Publications)

Rat Monoclonal Nucleoporin p62/NUP62 antibody. Suitable for IP, WB, ICC/IF and reacts with African green monkey, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUP62 aa 1-300.

View Alternative Names

Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, NUP62

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody [2A] (AB188413)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody [2A] (AB188413)

Immunofluorescent analysis of HeLa cells, labeling Nucleoporin p62/NUP62 with ab188413 at 1/400 dilution, followed by Alexa Fluor®488-conjugated goat anti-rat IgG secondary antibody at 1/500 dilution. HeLa cells were fixed with 3.7% formaldehyde and
permeabilized with 0.5% Triton X-100.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody [2A] (AB188413)

Immunofluorescent analysis of HeLa cells labeling Nucleoporin p62/NUP62 with ab188413 at 1/400 dilution, followed by Alexa Fluor®488-conjugated goat anti-rat IgG secondary antibody at 1/500 dilution. HeLa cells were fixed with 3.7% formaldehyde and
permeabilized with 0.5% Triton X-100.

Supplier Data

Western blot - Anti-Nucleoporin p62/NUP62 antibody [2A] (AB188413)

All lanes:

Western blot - Anti-Nucleoporin p62/NUP62 antibody [2A] (ab188413) at 1/500 dilution

All lanes:

HeLa nuclear membrane lysate

Secondary

All lanes:

Alkaline phosphatase-conjugated anti-rat IgG

Predicted band size: 53 kDa

Observed band size: 60 kDa

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Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human, African green monkey

Applications

ICC/IF, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human NUP62 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P37198

Epitope

aa 1-179 (FG-repeat region)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/400", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/400", "ICCIF-species-notes": "" }, "African green monkey": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/400", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

ab188413 was purified from serum-free culture medium of the hybridoma.

Storage buffer

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoporin p62 also known as NUP62 plays a significant role in transporting molecules across the nuclear envelope. This protein is a part of the nuclear pore complex which acts as a gateway for nucleocytoplasmic exchange. NUP62 has a molecular weight of approximately 62 kDa. It is expressed in the cell nucleus particularly at the nuclear envelope where it helps in the regulation of macromolecular traffic between the nucleus and cytoplasm.

Biological function summary

NUP62 is essential for maintaining the normal function of the nuclear pore complex. It forms a soluble subcomplex with other nucleoporins facilitating nuclear pore assembly. This protein contributes to the dynamic nature of the nuclear porins ensuring efficient and selective transport. NUP62 also interacts directly with nucleic acids and other transport receptors allowing for the precise regulation of nuclear-cytoplasmic exchange processes.

Pathways

NUP62 takes part in critical transport pathways such as the karyopherin-mediated import and export processes. It collaborates with importin-beta a transport receptor that binds to nuclear localization signals on cargo proteins. Through these pathways NUP62 ensures that essential proteins and RNAs are correctly swapped between the nucleus and the rest of the cell playing an important role in cellular homeostasis and gene expression regulation.

Defects in NUP62 have been linked to conditions like neurodegenerative diseases and certain cancers. Anomalies in its function can disrupt normal cellular communication and lead to pathological states. NUP62 interacts with proteins implicated in these conditions such as amyloid precursor protein in Alzheimer's disease indicating its importance in neuronal function. In cancer altered expression of NUP62 can contribute to the dysregulation of gene expression which is a hallmark of tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the nuclear pore complex (PubMed : 1915414). The N-terminal is probably involved in nucleocytoplasmic transport (PubMed : 1915414). The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex (PubMed : 1915414, PubMed : 24107630). Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation (PubMed : 24107630). It might be involved in protein recruitment to the centrosome after nuclear breakdown (PubMed : 24107630).

See full target information NUP62

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:3380 PubMed35697676

2022

NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda M Gleixner,Brandie Morris Verdone,Charlton G Otte,Eric N Anderson,Nandini Ramesh,Olivia R Shapiro,Jenna R Gale,Jocelyn C Mauna,Jacob R Mann,Katie E Copley,Elizabeth L Daley,Juan A Ortega,Maria Elena Cicardi,Evangelos Kiskinis,Julia Kofler,Udai B Pandey,Davide Trotti,Christopher J Donnelly

Nature neuroscience 24:1077-1088 PubMed34059832

2021

Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yen-Chen Lin,Meenakshi Sundaram Kumar,Nandini Ramesh,Eric N Anderson,Aivi T Nguyen,Boram Kim,Simon Cheung,Justin A McDonough,William C Skarnes,Rodrigo Lopez-Gonzalez,John E Landers,Nicolas L Fawzi,Ian R A Mackenzie,Edward B Lee,Jeffrey A Nickerson,David Grunwald,Udai B Pandey,Daryl A Bosco

Cell systems 10:133-155.e6 PubMed32027860

2020

A Global Screen for Assembly State Changes of the Mitotic Proteome by SEC-SWATH-MS.

Applications

Unspecified application

Species

Unspecified reactive species

Moritz Heusel,Max Frank,Mario Köhler,Sabine Amon,Fabian Frommelt,George Rosenberger,Isabell Bludau,Simran Aulakh,Monika I Linder,Yansheng Liu,Ben C Collins,Matthias Gstaiger,Ulrike Kutay,Ruedi Aebersold

Cellular and molecular life sciences : CMLS 76:2199-2216 PubMed30762072

2019

Nuclear pore protein TPR associates with lamin B1 and affects nuclear lamina organization and nuclear pore distribution.

Applications

Unspecified application

Species

Unspecified reactive species

Jindřiška Fišerová,Miloslava Maninová,Tomáš Sieger,Jana Uhlířová,Lenka Šebestová,Michaela Efenberková,Martin Čapek,Karel Fišer,Pavel Hozák

Developmental cell 43:141-156.e7 PubMed29065306

2017

Mitotic Disassembly of Nuclear Pore Complexes Involves CDK1- and PLK1-Mediated Phosphorylation of Key Interconnecting Nucleoporins.

Applications

Unspecified application

Species

Unspecified reactive species

Monika I Linder,Mario Köhler,Paul Boersema,Marion Weberruss,Cornelia Wandke,Joseph Marino,Caroline Ashiono,Paola Picotti,Wolfram Antonin,Ulrike Kutay

Journal of cell science 130:2066-2077 PubMed28476938

2017

Chromatin organization at the nuclear periphery as revealed by image analysis of structured illumination microscopy data.

Applications

Unspecified application

Species

Unspecified reactive species

Jindřiška Fišerová,Michaela Efenberková,Tomáš Sieger,Miloslava Maninová,Jana Uhlířová,Pavel Hozák

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Nucleoporin p62/NUP62 antibody [2A]

Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody [2A] (AB188413)

Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody [2A] (AB188413)

Western blot - Anti-Nucleoporin p62/NUP62 antibody [2A] (AB188413)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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