Anti-Nucleoporin p62/NUP62 antibody

• WB

Supplier Data

Western blot - Anti-Nucleoporin p62/NUP62 antibody (AB96134)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution

Lane 1:

NT2D1 whole cell lysates at 30 µg

Lane 2:

PC 3 whole cell lysates at 30 µg

Lane 3:

U87 MG whole cell lysates at 30 µg

Lane 4:

SK N SH whole cell lysates at 30 µg

Predicted band size: 53 kDa

false

• WB

Supplier Data

Western blot - Anti-Nucleoporin p62/NUP62 antibody (AB96134)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 53 kDa

false

• WB

Supplier Data

Western blot - Anti-Nucleoporin p62/NUP62 antibody (AB96134)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/5000 dilution

Lane 1:

Neuro2A whole cell lysates at 30 µg

Lane 2:

NIH 3T3 whole cell lysates at 30 µg

Lane 3:

Raw264.7 whole cell lysates at 30 µg

Lane 4:

C2C12 whole cell lysates at 30 µg

Predicted band size: 53 kDa

false

• WB

Supplier Data

Western blot - Anti-Nucleoporin p62/NUP62 antibody (AB96134)

Samples were separated by 12 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/5000 dilution

All lanes:

PC 12 whole cell lysate at 30 µg

Predicted band size: 53 kDa

false

• WB

CiteAb

Western blot - Anti-Nucleoporin p62/NUP62 antibody (AB96134)

Western Blotting using Anti-Nucleoporin p62/NUP62 antibody, ab96134. Publication image from Clift, D. et al., 2017, Cell, 29153837. Legend direct from paper.

Requirements for Trim-Away Efficiency and Specificity, Related to Figure 1, Figure 2, Figure 3, Figure 4, Figure 5, Figure 6, Figure 7(A) The indicated cell lines were electroporated with PBS, anti-ERK1 antibody, His-Lipoyl-TRIM21, or anti-ERK1 + His-Lipoyl-TRIM21 and whole cell lysates harvested 3 hours later for immunoblotting.(B and C) Normal human lung fibroblasts (NHLFs) were electroporated with the indicated proteins/antibodies and whole cell lysates harvested 3 hours later for immunoblotting.(D) HEK293T and HEK293T-mCherry-TRIM21 cell lines were electroporated with control IgG or an antibody directed against the Nucleoporin Nup98 (anti-Nup98), harvested 3 hours later and lysates immunoblotted for the indicated proteins. The Nup98 antibody was raised against the Nup98 N terminus, which is rich in phenylalanine-glycine (FG) repeats. The FG-repeats are shared by several other nucleoporins collectively known as the FG Nups (Brohawn et al., 2009). Asterisks show additional bands recognized by the anti-Nup98 antibody which are the same size as Nup214 and Nup62 recognized by the Mab414 and anti-Nup62 antibodies respectively. Consistent with non-specific binding, Trim-Away using the anti-Nup98 N-terminal antibody also triggered degradation of Nup62 and Nup214. Other nucleoporins Nup358 and Nup153 were not degraded, suggesting that co-depletion was not the result of degradation of the entire nuclear pore complex.

false