Rabbit Polyclonal Nucleoporin p62/NUP62 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUP62 aa 250 to C-terminus.
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- Molecular metabolism 64:1015672022Dual specificity phosphatase 1 attenuates inflammation-induced cardiomyopathy by improving mitophagy and mitochondrial metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Tan,Yue Zhang,Jing He,Feng Wu,Di Wu,Nengxian Shi,Weifeng Liu,Ziying Li,Wenqian Liu,Hao Zhou,Wenting ChenPubMed 35944900
- FEBS open bio 10:2021-20392020miRNA profiling of primate cervicovaginal lavage and extracellular vesicles reveals miR-186-5p as a potential antiretroviral factor in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZezhou Zhao et. al.PubMed 33017084
- The journal of gene medicine 22:e32002020miR-129-5p attenuates hypoxia-induced apoptosis in rat H9c2 cardiomyocytes by activating autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjia Li,Yanping Ren,Tianyu Meng,Wei Yang,Wei ZhangPubMed 32298509
- Experimental and therapeutic medicine 17:1884-18902019Inhibition of HIF-1α restrains fracture healing via regulation of autophagy in a rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunjie Qiao,Jiang Huang,Meng Zhou,Guanglei Cao,Huiliang ShenPubMed 30783464
- Cell 171:1692-1706.e182017A Method for the Acute and Rapid Degradation of Endogenous Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDean Clift et. al.PubMed 29153837
- Journal of immunology (Baltimore, Md. : 1950) 197:3086-30982016Global Analysis of O-GlcNAc Glycoproteins in Activated Human T Cells.
- International journal of molecular medicine 37:679-892016HMGB1 induces apoptosis and EMT in association with increased autophagy following H/R injury in cardiomyocytes.
- Molecular and cellular biology 35:2658-722015Hypoxia Signaling Cascade for Erythropoietin Production in Hepatocytes.
- PLoS pathogens 11:e10047762015Impact of Leishmania infection on host macrophage nuclear physiology and nucleopore complex integrity.
- Cell death & disease 6:e16242015DRAM1 regulates apoptosis through increasing protein levels and lysosomal localization of BAX.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ-J Guan et. al.PubMed 25633293
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NUP62 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P37198
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Expected | Expected |
| Cow | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Essential component of the nuclear pore complex (PubMed:1915414). The N-terminal is probably involved in nucleocytoplasmic transport (PubMed:1915414). The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex (PubMed:1915414, PubMed:24107630). Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation (PubMed:24107630). It might be involved in protein recruitment to the centrosome after nuclear breakdown (PubMed:24107630).
Alternative names
Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, NUP62
Recommended products
Rabbit Polyclonal Nucleoporin p62/NUP62 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUP62 aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NUP62 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P37198
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nucleoporin p62 also known as NUP62 plays a significant role in transporting molecules across the nuclear envelope. This protein is a part of the nuclear pore complex which acts as a gateway for nucleocytoplasmic exchange. NUP62 has a molecular weight of approximately 62 kDa. It is expressed in the cell nucleus particularly at the nuclear envelope where it helps in the regulation of macromolecular traffic between the nucleus and cytoplasm.
- Biological function summary
NUP62 is essential for maintaining the normal function of the nuclear pore complex. It forms a soluble subcomplex with other nucleoporins facilitating nuclear pore assembly. This protein contributes to the dynamic nature of the nuclear porins ensuring efficient and selective transport. NUP62 also interacts directly with nucleic acids and other transport receptors allowing for the precise regulation of nuclear-cytoplasmic exchange processes.
- Pathways
NUP62 takes part in critical transport pathways such as the karyopherin-mediated import and export processes. It collaborates with importin-beta a transport receptor that binds to nuclear localization signals on cargo proteins. Through these pathways NUP62 ensures that essential proteins and RNAs are correctly swapped between the nucleus and the rest of the cell playing an important role in cellular homeostasis and gene expression regulation.
- Associated diseases and disorders
Defects in NUP62 have been linked to conditions like neurodegenerative diseases and certain cancers. Anomalies in its function can disrupt normal cellular communication and lead to pathological states. NUP62 interacts with proteins implicated in these conditions such as amyloid precursor protein in Alzheimer's disease indicating its importance in neuronal function. In cancer altered expression of NUP62 can contribute to the dysregulation of gene expression which is a hallmark of tumorigenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134)
10% SDS-PAGE.
All lanes: Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1: Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 30 µg
Lane 2: GL261 whole cell lysate at 30 µg
Lane 3: C8D30 whole cell lysate at 30 µg
Lane 4: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Lane 5: BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate at 30 µg
Lane 6: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg
Lane 7: C2C12 (Mouse myoblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 53 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody (ab96134)
ab96134, at a 1/200 dilution, staining Nucleoporin p62/NUP62 in paraformaldehyde fixed A431 by Immunofluorescence analysis.
Lower image: merged with DNA probe.
Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134)
10% SDS-PAGE.
All lanes: Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Lane 2: Rat2 (Rat fibroblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoporin p62/NUP62 antibody (ab96134)
Paraffin-embedded mouse fore brain tissue stained for Nucleoporin p62/NUP62 using ab96134 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134)
10% SDS PAGE
All lanes: Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1: A431 whole cell lysate at 30 µg
Lane 2: H1299 whole cell lysate at 30 µg
Predicted band size: 53 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody (ab96134)
Immunofluorescence analysis of A431 cells, staining Nucleoporin p62/NUP62 with ab96134 at 1/200 dilution, demonstrating punctate nuclear staining.
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