Rabbit Recombinant Monoclonal Nucleoporin p62/NUP62 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human NUP62.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836), expandable thumbnail](https://content.abcam.com/products/images/nucleoporin-p62-nup62-antibody-hl1226-bsa-and-azide-free-ab313836--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440706.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836)")
![Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836), expandable thumbnail](https://content.abcam.com/products/images/nucleoporin-p62-nup62-antibody-hl1226-bsa-and-azide-free-ab313836--western-blot-img419117.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836)")
![Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836), expandable thumbnail](https://content.abcam.com/products/images/nucleoporin-p62-nup62-antibody-hl1226-bsa-and-azide-free-ab313836--western-blot-img419116.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836)")
![Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836), expandable thumbnail](https://content.abcam.com/products/images/nucleoporin-p62-nup62-antibody-hl1226-bsa-and-azide-free-ab313836--western-blot-img419115.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human NUP62. Database link P37198
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Expected |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Mouse | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Rat | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Essential component of the nuclear pore complex (PubMed:1915414). The N-terminal is probably involved in nucleocytoplasmic transport (PubMed:1915414). The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex (PubMed:1915414, PubMed:24107630). Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation (PubMed:24107630). It might be involved in protein recruitment to the centrosome after nuclear breakdown (PubMed:24107630).
Additional Targets
Nup62, Nup62
Alternative names
Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, NUP62
Recommended products
Rabbit Recombinant Monoclonal Nucleoporin p62/NUP62 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human NUP62.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human NUP62. Database link P37198
- Carrier free
- Yes
- Clone number
- HL1226
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nucleoporin p62 also known as NUP62 plays a significant role in transporting molecules across the nuclear envelope. This protein is a part of the nuclear pore complex which acts as a gateway for nucleocytoplasmic exchange. NUP62 has a molecular weight of approximately 62 kDa. It is expressed in the cell nucleus particularly at the nuclear envelope where it helps in the regulation of macromolecular traffic between the nucleus and cytoplasm.
- Biological function summary
NUP62 is essential for maintaining the normal function of the nuclear pore complex. It forms a soluble subcomplex with other nucleoporins facilitating nuclear pore assembly. This protein contributes to the dynamic nature of the nuclear porins ensuring efficient and selective transport. NUP62 also interacts directly with nucleic acids and other transport receptors allowing for the precise regulation of nuclear-cytoplasmic exchange processes.
- Pathways
NUP62 takes part in critical transport pathways such as the karyopherin-mediated import and export processes. It collaborates with importin-beta a transport receptor that binds to nuclear localization signals on cargo proteins. Through these pathways NUP62 ensures that essential proteins and RNAs are correctly swapped between the nucleus and the rest of the cell playing an important role in cellular homeostasis and gene expression regulation.
- Associated diseases and disorders
Defects in NUP62 have been linked to conditions like neurodegenerative diseases and certain cancers. Anomalies in its function can disrupt normal cellular communication and lead to pathological states. NUP62 interacts with proteins implicated in these conditions such as amyloid precursor protein in Alzheimer's disease indicating its importance in neuronal function. In cancer altered expression of NUP62 can contribute to the dysregulation of gene expression which is a hallmark of tumorigenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836), expandable thumbnail](https://content.abcam.com/products/images/nucleoporin-p62-nup62-antibody-hl1226-bsa-and-azide-free-ab313836--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440706.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836)
Paraffin-embedded rat brain tissue stained for Nucleoporin p62 protein at nuclear envelope with ab313836 at a 1/100 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
![Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836), expandable thumbnail](https://content.abcam.com/products/images/nucleoporin-p62-nup62-antibody-hl1226-bsa-and-azide-free-ab313836--western-blot-img419117.jpg "Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836)")
Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836)
All lanes: Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836) at 1/5000 dilution
Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell extract at 30 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 4: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 53 kDa
![Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836), expandable thumbnail](https://content.abcam.com/products/images/nucleoporin-p62-nup62-antibody-hl1226-bsa-and-azide-free-ab313836--western-blot-img419116.jpg "Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836)")
Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836)
All lanes: Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836) at 1/5000 dilution
Lane 1: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Lane 2: Rat2 (Rat fibroblast cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 53 kDa
![Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836), expandable thumbnail](https://content.abcam.com/products/images/nucleoporin-p62-nup62-antibody-hl1226-bsa-and-azide-free-ab313836--western-blot-img419115.jpg "Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836)")
Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836)
All lanes: Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836) at 1/5000 dilution
Lane 1: Neuro-2a (Mouse neuroblastoma cell line) whole cell extract at 30 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell extract at 30 µg
Lane 3: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg
Lane 4: C2C12 (Mouse myoblast cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 53 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com