AB313836

Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal Nucleoporin p62/NUP62 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human NUP62.

View Alternative Names

Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, NUP62, Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, Nup62, Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, Nup62

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836)

Paraffin-embedded rat brain tissue stained for Nucleoporin p62 protein at nuclear envelope with ab313836 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836)

All lanes:

Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836) at 1/5000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (Human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836)

All lanes:

Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836) at 1/5000 dilution

Lane 1:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg

Lane 2:

Rat2 (Rat fibroblast cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 53 kDa

true

Supplier Data

Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836)

All lanes:

Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (ab313836) at 1/5000 dilution

Lane 1:

Neuro-2a (Mouse neuroblastoma cell line) whole cell extract at 30 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell extract at 30 µg

Lane 3:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg

Lane 4:

C2C12 (Mouse myoblast cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 53 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1226

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat, Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NUP62.

P37198

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoporin p62 also known as NUP62 plays a significant role in transporting molecules across the nuclear envelope. This protein is a part of the nuclear pore complex which acts as a gateway for nucleocytoplasmic exchange. NUP62 has a molecular weight of approximately 62 kDa. It is expressed in the cell nucleus particularly at the nuclear envelope where it helps in the regulation of macromolecular traffic between the nucleus and cytoplasm.

Biological function summary

NUP62 is essential for maintaining the normal function of the nuclear pore complex. It forms a soluble subcomplex with other nucleoporins facilitating nuclear pore assembly. This protein contributes to the dynamic nature of the nuclear porins ensuring efficient and selective transport. NUP62 also interacts directly with nucleic acids and other transport receptors allowing for the precise regulation of nuclear-cytoplasmic exchange processes.

Pathways

NUP62 takes part in critical transport pathways such as the karyopherin-mediated import and export processes. It collaborates with importin-beta a transport receptor that binds to nuclear localization signals on cargo proteins. Through these pathways NUP62 ensures that essential proteins and RNAs are correctly swapped between the nucleus and the rest of the cell playing an important role in cellular homeostasis and gene expression regulation.

Defects in NUP62 have been linked to conditions like neurodegenerative diseases and certain cancers. Anomalies in its function can disrupt normal cellular communication and lead to pathological states. NUP62 interacts with proteins implicated in these conditions such as amyloid precursor protein in Alzheimer's disease indicating its importance in neuronal function. In cancer altered expression of NUP62 can contribute to the dysregulation of gene expression which is a hallmark of tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the nuclear pore complex (PubMed : 1915414). The N-terminal is probably involved in nucleocytoplasmic transport (PubMed : 1915414). The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex (PubMed : 1915414, PubMed : 24107630). Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation (PubMed : 24107630). It might be involved in protein recruitment to the centrosome after nuclear breakdown (PubMed : 24107630).

See full target information NUP62

Additional targets

Nup62,Nup62

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836)

Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836)

Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836)

Western blot - Anti-Nucleoporin p62/NUP62 antibody [HL1226] - BSA and Azide free (AB313836)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise