AB183480

Anti-Nucleoporin p62/NUP62 (phospho T269 + S272) antibody

Name: Anti-Nucleoporin p62/NUP62 (phospho T269 + S272) antibody (ab183480) | Abcam
Brand: Abcam Limited
SKU: AB183480

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(1 Publication)

Rabbit Polyclonal Nucleoporin p62/NUP62 phospho S272 + T269 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Nuclear pore glycoprotein p62 phospho S272 + T269.

View Alternative Names

Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, NUP62

1 Images

Supplier Data

Western blot - Anti-Nucleoporin p62/NUP62 (phospho T269 + S272) antibody (AB183480)

All lanes:

Western blot - Anti-Nucleoporin p62/NUP62 (phospho T269 + S272) antibody (ab183480) at 1/1000 dilution

Lane 1:

Jurkat cell lysate

Lane 2:

Jurkat cell lysate with immunizing phosphopeptide

Predicted band size: 53 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human Nuclear pore glycoprotein p62 phospho S272 + T269. The exact immunogen used to generate this antibody is proprietary information.

P37198

Specificity

ab183480 is specific for the ~62kDa Nucleoporin p62 protein phosphorylated at Thr269 and Ser272. Immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen but not by the corresponding dephosphopeptide

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Primates": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab183480 is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage buffer

pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoporin p62 also known as NUP62 plays a significant role in transporting molecules across the nuclear envelope. This protein is a part of the nuclear pore complex which acts as a gateway for nucleocytoplasmic exchange. NUP62 has a molecular weight of approximately 62 kDa. It is expressed in the cell nucleus particularly at the nuclear envelope where it helps in the regulation of macromolecular traffic between the nucleus and cytoplasm.

Biological function summary

NUP62 is essential for maintaining the normal function of the nuclear pore complex. It forms a soluble subcomplex with other nucleoporins facilitating nuclear pore assembly. This protein contributes to the dynamic nature of the nuclear porins ensuring efficient and selective transport. NUP62 also interacts directly with nucleic acids and other transport receptors allowing for the precise regulation of nuclear-cytoplasmic exchange processes.

Pathways

NUP62 takes part in critical transport pathways such as the karyopherin-mediated import and export processes. It collaborates with importin-beta a transport receptor that binds to nuclear localization signals on cargo proteins. Through these pathways NUP62 ensures that essential proteins and RNAs are correctly swapped between the nucleus and the rest of the cell playing an important role in cellular homeostasis and gene expression regulation.

Defects in NUP62 have been linked to conditions like neurodegenerative diseases and certain cancers. Anomalies in its function can disrupt normal cellular communication and lead to pathological states. NUP62 interacts with proteins implicated in these conditions such as amyloid precursor protein in Alzheimer's disease indicating its importance in neuronal function. In cancer altered expression of NUP62 can contribute to the dysregulation of gene expression which is a hallmark of tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the nuclear pore complex (PubMed : 1915414). The N-terminal is probably involved in nucleocytoplasmic transport (PubMed : 1915414). The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex (PubMed : 1915414, PubMed : 24107630). Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation (PubMed : 24107630). It might be involved in protein recruitment to the centrosome after nuclear breakdown (PubMed : 24107630).

See full target information Nuclear pore glycoprotein p62 phospho S272 + T269

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 30:29 PubMed40045239

2025

CDC20 protects the heart from doxorubicin-induced cardiotoxicity by modulating CCDC69 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyu Feng,Ningning Zhang,Liang Wang,Xumin Guan,Yunpeng Xie,Yun-Long Xia

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Nucleoporin p62/NUP62 (phospho T269 + S272) antibody

Western blot - Anti-Nucleoporin p62/NUP62 (phospho T269 + S272) antibody (AB183480)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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