Rabbit Polyclonal Nucleoporin p62/NUP62 phospho S272 + T269 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Nuclear pore glycoprotein p62 phospho S272 + T269.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Nuclear pore glycoprotein p62 phospho S272 + T269. The exact immunogen used to generate this antibody is proprietary information. Database link P37198
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Primates | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Primates | Dilution info - | Notes - |
Associated Products
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Target data
Function
Essential component of the nuclear pore complex (PubMed:1915414). The N-terminal is probably involved in nucleocytoplasmic transport (PubMed:1915414). The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex (PubMed:1915414, PubMed:24107630). Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation (PubMed:24107630). It might be involved in protein recruitment to the centrosome after nuclear breakdown (PubMed:24107630).
Alternative names
Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, NUP62
Recommended products
Rabbit Polyclonal Nucleoporin p62/NUP62 phospho S272 + T269 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Nuclear pore glycoprotein p62 phospho S272 + T269.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Nuclear pore glycoprotein p62 phospho S272 + T269. The exact immunogen used to generate this antibody is proprietary information. Database link P37198
- Purification technique
- Affinity purification Immunogen
- Specificity
ab183480 is specific for the ~62kDa Nucleoporin p62 protein phosphorylated at Thr269 and Ser272. Immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen but not by the corresponding dephosphopeptide
- Concentration
- Purification notes
ab183480 is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nucleoporin p62 also known as NUP62 plays a significant role in transporting molecules across the nuclear envelope. This protein is a part of the nuclear pore complex which acts as a gateway for nucleocytoplasmic exchange. NUP62 has a molecular weight of approximately 62 kDa. It is expressed in the cell nucleus particularly at the nuclear envelope where it helps in the regulation of macromolecular traffic between the nucleus and cytoplasm.
- Biological function summary
NUP62 is essential for maintaining the normal function of the nuclear pore complex. It forms a soluble subcomplex with other nucleoporins facilitating nuclear pore assembly. This protein contributes to the dynamic nature of the nuclear porins ensuring efficient and selective transport. NUP62 also interacts directly with nucleic acids and other transport receptors allowing for the precise regulation of nuclear-cytoplasmic exchange processes.
- Pathways
NUP62 takes part in critical transport pathways such as the karyopherin-mediated import and export processes. It collaborates with importin-beta a transport receptor that binds to nuclear localization signals on cargo proteins. Through these pathways NUP62 ensures that essential proteins and RNAs are correctly swapped between the nucleus and the rest of the cell playing an important role in cellular homeostasis and gene expression regulation.
- Associated diseases and disorders
Defects in NUP62 have been linked to conditions like neurodegenerative diseases and certain cancers. Anomalies in its function can disrupt normal cellular communication and lead to pathological states. NUP62 interacts with proteins implicated in these conditions such as amyloid precursor protein in Alzheimer's disease indicating its importance in neuronal function. In cancer altered expression of NUP62 can contribute to the dysregulation of gene expression which is a hallmark of tumorigenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Nucleoporin p62/NUP62 (phospho T269 + S272) antibody (ab183480)
All lanes: Western blot - Anti-Nucleoporin p62/NUP62 (phospho T269 + S272) antibody (ab183480) at 1/1000 dilution
Lane 1: Jurkat cell lysate
Lane 2: Jurkat cell lysate with immunizing phosphopeptide
Predicted band size: 53 kDa
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