AB309553

Anti-Nucleoprotein antibody [EPR24334-11] - BSA and Azide free (Capture)

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for sELISA and reacts with SARS-CoV-2 samples.

View Alternative Names

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N

1 Images

Sandwich ELISA - Anti-Nucleoprotein antibody [EPR24334-11] - BSA and Azide free (Capture) (AB309553)

sELISA

Supplier Data

Sandwich ELISA - Anti-Nucleoprotein antibody [EPR24334-11] - BSA and Azide free (Capture) (AB309553)

Sandwich ELISA of ab309552 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24334-11

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

style

left-column

Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 Nucleoprotein often referred to as the N protein plays a central role in the life cycle of the virus. It has a molecular mass of approximately 45 to 50 kDa and is encoded by the N gene. The N protein is expressed in the cytoplasm of infected cells. It forms the viral ribonucleoprotein complex by binding to the viral RNA genome ensuring its proper packaging into new particles. The N protein supports viral replication and assembly through interactions with other viral proteins.

Biological function summary

The N protein interacts with several host cellular components to facilitate viral replication. It is not part of a large complex but forms homodimers which are essential for its function. Through these interactions the N protein disrupts normal cellular processes to favor viral replication. The N protein can modulate the host's immune response by interfering with signal transduction pathways this helps the virus evade detection.

Pathways

Many cell processes are hijacked by the N protein. It is involved in the viral replication and transcription complex which includes interacting with the host's translation machinery. The N protein associates with proteins like RNA polymerase to ensure efficient viral genome replication. It also affects the intracellular transport and assembly pathways of the virus critical for the production of infectious virions.

The N protein has a significant role in COVID-19. Its presence is often used as a marker for infection and is a target for diagnostic assays. The N protein can trigger an excessive immune response leading to cytokine storms associated with severe acute respiratory distress syndrome (ARDS) in COVID-19 patients. It is connected to other viral proteins such as the spike protein which is involved in the virus's ability to enter host cells compounding its impact on disease progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed : 33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed : 34901782, PubMed : 36534661).. May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed : 35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed : 35921414).. May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.

See full target information N

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nucleoprotein antibody [EPR24334-11] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-Nucleoprotein antibody [EPR24334-11] - BSA and Azide free (Capture) (AB309553)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise