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AB324964

Anti-Nucleoprotein Crimean-Congo hemorrhagic fever virus antibody [BA11]

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Mouse Recombinant Monoclonal NCAP antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Crimean-Congo hemorrhagic fever virus (isolate C68031), Recombinant fragment - Crimean-Congo hemorrhagic fever virus (isolate C68031) samples.

View Alternative Names

Nucleoprotein, Nucleocapsid protein, Protein N

2 Images
Indirect ELISA - Anti-Nucleoprotein Crimean-Congo hemorrhagic fever virus antibody [BA11] (AB324964)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Nucleoprotein Crimean-Congo hemorrhagic fever virus antibody [BA11] (AB324964)

Indirect ELISA analysis of ab324964 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution dilution.

Antigen : Glycoprotein N Crimean-Congo hemorrhagic fever virus, Nucleoprotein Crimean-Congo hemorrhagic fever virus.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Nucleoprotein Crimean-Congo hemorrhagic fever virus antibody [BA11] (AB324964)
  • WB

Supplier Data

Western blot - Anti-Nucleoprotein Crimean-Congo hemorrhagic fever virus antibody [BA11] (AB324964)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Nucleoprotein Crimean-Congo hemorrhagic fever virus antibody [BA11] (ab324964) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his-tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Glycoprotein N Crimean-Coo hemorrhagic fever virus expression vector containing a myc-his-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Nucleoprotein Crimean-Coo hemorrhagic fever virus expression vector containing a myc-his-tag whole cell lysate at 1 µg

Lane 4:

293T cells transfected with a Glycoprotein C Crimean-Coo hemorrhagic fever virus expression vector containing a myc-his-tag whole cell lysate at 4 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 53 kDa,36 kDa

false

Exposure time: 4s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BA11

Isotype

IgG1

Carrier free

No

Reacts with

Crimean-Congo hemorrhagic fever virus (isolate C68031)

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Crimean-Congo hemorrhagic fever virus (isolate C68031)": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Crimean-Congo hemorrhagic fever virus (isolate C68031)": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "62.5 ng/mL", "IELISA-species-notes": "<p></p>" }, "Transfected cell lysate - Crimean-Congo hemorrhagic fever virus (isolate C68031)": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds dsRNA and ssRNA and probably participates in the packaging of viral genome (By similarity). In the dsRNA binding mode, the nucleocapsid protein specifically binds to the vRNA panhandle secondary structure formed at the termini of viral genome (By similarity). Does not discriminate between viral and nonviral RNAs through ssRNA binding mode (By similarity). Displays dsDNA endonuclease activity that is sequence non-specific (By similarity).
See full target information N
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com