JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB220753

Anti-Nucleoside Diphosphate Kinase 7 antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal Nucleoside Diphosphate Kinase 7 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Nucleoside diphosphate kinase 7 aa 150-300.

View Alternative Names

Nucleoside diphosphate kinase 7, NDK7, NDP kinase 7, Putative 3'-5'-DNA exonuclease NDK7, Serine protein kinase NME7, nm23-H7, NME7

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside Diphosphate Kinase 7 antibody (AB220753)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside Diphosphate Kinase 7 antibody (AB220753)

Immunofluorescent analysis of paraformaldehyde-fixed, Triton X-100 permeabilized U-251 MG cells labeling Nucleoside Diphosphate Kinase 7 with ab220753 at 4 μg/mL dilution (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Nucleoside diphosphate kinase 7 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5B8

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoside Diphosphate Kinase 7 also known as NME7 plays an important role in the catalysis of the transfer of gamma phosphate from nucleoside triphosphates to nucleoside diphosphates. This kinase part of the NM23 family comprises an approximate mass of 41 kDa and resides primarily in the human cytosol. Expression of NME7 occurs broadly in various tissues with notable amounts in the brain and liver. The presence of alternate names like NDPK 7 within scientific literature highlights its established relevance in the nucleoside diphosphate kinase activity field.
Biological function summary

Nucleoside diphosphate kinase facilitates regulation of cellular processes including cell proliferation differentiation and signal transduction. Though it does not traditionally form large protein complexes its actions link it indirectly to several molecular assemblies through substrate and product interactions. NME7 demonstrates additional roles in microtubule organization essential for cellular structural maintenance. Its ATP-binding and transfer capabilities make it an important player in maintaining cellular nucleotide balance.

Pathways

NME7 engages in metabolic and signal transduction pathways that manage nucleotide synthesis and energy balance. In the purine metabolism pathway it functions alongside enzymes like guanylate kinase. NME7 also plays a role in the microtubule-associated regulation pathway affecting cell division and stability. By interacting with proteins such as tubulin it further integrates within the cytoskeletal dynamics system important for cell cycle progression and mitosis.

Nucleoside diphosphate kinase 7 implicates itself in neurodegenerative diseases and certain types of cancer. Its function in nucleotide balance and cell proliferation connects it to pathways disrupted in these diseases. Misregulation of NME7 has ties to Alzheimer's disease through its interaction with proteins involved in apoptosis and microtubule assembly like Tau protein. Additional links to cancer progression stem from alterations in its kinase activity often resulting in unchecked cellular growth in malignancies. Understanding these connections may present therapeutic opportunities targeting NME7-related pathways.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Possesses an intrinsic kinase activity (PubMed : 24807905, PubMed : 34764205). Phosphorylates GSK3B at 'Ser-9', leading to the activation of Wnt/beta-catenin signaling (PubMed : 34764205). Additionally, exhibits a 3'-5'-DNA exonuclease activity that removes single nucleotides from the 3' terminus of single-stranded DNA substrates and digests overhanging mismatched 3' termini from double-stranded DNA substrates, possibly participating in DNA nucleolytic processing (PubMed : 16313181). In vitro, does not seem to have nucleoside diphosphate kinase activity (PubMed : 16313181, PubMed : 24807905). Functional component of the gamma-tubulin ring complex, implicated in the regulation of its microtubule-nucleating activity in centrosomes, in a kinase activity-dependent manner (PubMed : 24807905). May maintain primary cilium assembly and ciliary microtubule stability (PubMed : 39824631). Part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, required for motile cilia beating (PubMed : 36191189).
See full target information Nucleoside diphosphate kinase 7

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Genes 12: PubMed34356103

2021

Heterozygous Mutation Affects Glucose Tolerance in Male Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lucie Šedová,Jan Prochazka,Dagmar Zudová,Běla Bendlová,Josef Včelák,Radislav Sedlacek,Ondřej Šeda

International journal of molecular sciences 22: PubMed33916973

2021

Semi-Lethal Primary Ciliary Dyskinesia in Rats Lacking the Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Lucie Šedová,Ivana Buková,Pavla Bažantová,Silvia Petrezsélyová,Jan Prochazka,Elena Školníková,Dagmar Zudová,Josef Včelák,Pavol Makovický,Běla Bendlová,Ondřej Šeda,Radislav Sedlacek

Oncology letters 16:6573-6581 PubMed30405797

2018

Therapeutic effects of lenvatinib in combination with rAd-p53 for the treatment of non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Renzhi Yu,Minghuan Wang,Xiuli Zhu,Zhe Sun,Aiying Jiang,Huixin Yao
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com