AB244255

Anti-Nucleoside phosphorylase antibody

Name: Anti-Nucleoside phosphorylase antibody (ab244255) | Abcam
Brand: Abcam Limited
SKU: AB244255
Price: 630 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Nucleoside phosphorylase antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PNP aa 1-200.

View Alternative Names

NP, PNP, Purine nucleoside phosphorylase, Inosine phosphorylase, Inosine-guanosine phosphorylase

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoside phosphorylase antibody (AB244255)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoside phosphorylase antibody (AB244255)

Formalin-fixed, paraffin-embedded human testis tissue stained for Nucleoside phosphorylase with ab244255 at a 1/200 dilution in immunohistochemical analysis.

Low expression as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoside phosphorylase antibody (AB244255)

Formalin-fixed, paraffin-embedded human epididymis tissue stained for Nucleoside phosphorylase with ab244255 at a 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside phosphorylase antibody (AB244255)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside phosphorylase antibody (AB244255)

PFA fixed, Triton X-100 permeabilized RPTEC TERT1 (Human cell line) cells labeling Nucleoside phosphorylase using ab244255 at 4 μg/ml (green) in ICC/IF.

Unknown

Western blot - Anti-Nucleoside phosphorylase antibody (AB244255)

All lanes:

Western blot - Anti-Nucleoside phosphorylase antibody (ab244255) at 0.4 µg/mL

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (Rat cell line) cell lysate

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PNP aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P00491

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoside phosphorylase also known as PNP or purine nucleoside phosphorylase is an enzyme with a vital mechanical role in nucleoside metabolism. The enzyme facilitates the phosphorolytic cleavage of the glycosidic bond in nucleosides producing ribose 1-phosphate and free bases such as guanine and hypoxanthine. This catalytic function plays an important part in nucleotide salvage pathways. PNP has a molecular mass of approximately 32000 Daltons and shows expression predominantly in the lymphoid tissues including the thymus and spleen.

Biological function summary

Nucleoside phosphorylase is important in maintaining nucleotide homeostasis. It operates as a monomer or part of a homotrimeric complex which allows it to efficiently catalyze its reactions in purine metabolism. The absence or dysfunction of PNP results in the accumulation of nucleosides and diminished levels of nucleotide pools which can severely hamper DNA replication and repair.

Pathways

Nucleoside phosphorylase plays an integral role in the purine salvage pathway which is vital for recycling purines to form new nucleotides. It works alongside other enzymes such as adenine phosphoribosyltransferase to conserve energy by recycling purines. This pathway connects closely with the hypoxanthine-guanine phosphoribosyltransferase (HGPRT) pathway showing how PNP is intertwined in broader nucleotide biosynthesis and degradation processes.

Deficiencies in nucleoside phosphorylase correlate strongly with immunodeficiencies notably purine nucleoside phosphorylase deficiency (PNP deficiency) which leads to compromised T-cell immunity. This condition can result in recurrent infections and developmental delay. Additionally the enzyme's dysregulation associates with certain leukemias where altered nucleotide pools contribute to the proliferation of malignant cells. In these contexts PNP interacts with proteins central to these disorders including those involved in purine metabolism placing it at a critical junction for potential therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate (PubMed : 23438750, PubMed : 3029074, PubMed : 9305964). Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine (PubMed : 9305964).

See full target information PNP

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 13:2922-2937 PubMed37559979

2023

Indisulam exerts anticancer effects via modulation of transcription, translation and alternative splicing on human cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihui Dou,Xuetian Zhang,Wei Su,Taotao Zhang,Fei Ye,Dapeng Zhao,Xiaohua Chen,Qiang Li,Hong Zhang,Cuixia Di

Frontiers in cell and developmental biology 9:636802 PubMed33869182

2021

Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoting Chen,Mian Wu,Nan Liang,Junxi Lu,Shen Qu,Haibing Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Nucleoside phosphorylase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoside phosphorylase antibody (AB244255)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoside phosphorylase antibody (AB244255)

Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside phosphorylase antibody (AB244255)

Western blot - Anti-Nucleoside phosphorylase antibody (AB244255)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise