Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - BSA and Azide free (AB250597)

This data was developed using ab182155, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of U87-MG cells, staining Nucleoside triphosphate phosphohydrolase (red) with ab182155 at 1/100 dilution. An Alexa Fluor®555-conjugated goat anti rabbit IgG was used as a secondary antibody. Nuclei were counterstained with DAPI (blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - BSA and Azide free (AB250597)

This data was developed using ab182155, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of A549 cells, staining Nucleoside triphosphate phosphohydrolase (green) with ab182155 at 1/100 dilution. An Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody. Nuclei were counterstained with DAPI (blue).

• IP

Supplier Data

Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - BSA and Azide free (AB250597)

This data was developed using ab182155, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate (lane 1) or negative control (lane 2), labeling Nucleoside triphosphate phosphohydrolase immunoprecipitated using ab182155 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (<a href='/en-us/products/primary-antibodies/nucleoside-triphosphate-phosphohydrolase-antibody-epr14325-50-n-terminal-ab182155'>ab182155</a>)

Predicted band size: 21 kDa

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• WB

Supplier Data

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - BSA and Azide free (AB250597)

This data was developed using ab182155, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (<a href='/en-us/products/primary-antibodies/nucleoside-triphosphate-phosphohydrolase-antibody-epr14325-50-n-terminal-ab182155'>ab182155</a>) at 1/20000 dilution

Lane 1:

U87-MG cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

• WB

Lab

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - BSA and Azide free (AB250597)

This data was developed using ab182155, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab182155 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.

ab182155 Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal was shown to specifically react with Nucleoside triphosphate phosphohydrolase in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266397 (knockout cell lysate ab258083) was used. Wild-type and Nucleoside triphosphate phosphohydrolase knockout samples were subjected to SDS-PAGE. ab182155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325-50] - N-terminal (<a href='/en-us/products/primary-antibodies/nucleoside-triphosphate-phosphohydrolase-antibody-epr14325-50-n-terminal-ab182155'>ab182155</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

NTPCR knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human NTPCR (Nucleoside triphosphate phosphohydrolase) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ntpcr-nucleoside-triphosphate-phosphohydrolase-knockout-hek-293t-cell-line-ab266397'>ab266397</a>)

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false