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Rabbit Recombinant Monoclonal Nucleoside triphosphate phosphohydrolase antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (AB182154), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (AB182154), expandable thumbnail
  • Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (AB182154), expandable thumbnail
  • Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (AB182154), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/230
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Has nucleotide phosphatase activity towards ATP, GTP, CTP, TTP and UTP. Hydrolyzes nucleoside diphosphates with lower efficiency.

Alternative names

C1orf57, NTPCR, Cancer-related nucleoside-triphosphatase, NTPase, Nucleoside triphosphate phosphohydrolase

Recommended products

Rabbit Recombinant Monoclonal Nucleoside triphosphate phosphohydrolase antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR14325
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Nucleoside triphosphate phosphohydrolase often called NTPase is an enzyme responsible for the hydrolysis of nucleoside triphosphates. It plays an important role in converting nucleoside triphosphates to nucleoside diphosphates with the release of inorganic phosphate. This process is important as it regulates the availability of nucleotides for various biological functions. NTPase typically with a mass of approximately 70 kDa is expressed in various tissues but shows high activity in tissues with rapid cell division. It is particularly studied in cellular lines such as HEK293T where it assists in the investigation of cellular mechanisms.

Biological function summary

This enzyme participates in critical cellular processes by managing the concentration of nucleoside triphosphates. Its activity influences DNA and RNA synthesis signal transduction and energy transfer. NTPase does not generally form complexes with other proteins but may interact with other molecules transiently during its catalytic action. This transient nature allows it to act efficiently where nucleoside triphosphate function is necessary.

Pathways

NTPase influences multiple biochemical pathways important for cell viability and function. It plays a role in the nucleotide metabolism pathway which is essential for DNA and RNA biosynthesis. Moreover its involvement in the energy transfer pathway ties it to proteins such as myosin and kinases which require energy from ATP hydrolysis to function properly. These interactions underline its importance in cellular physiology.

Associated diseases and disorders

Disruptions in NTPase activity have been linked to mitochondrial disorders and certain cancers. Abnormal levels of nucleoside triphosphates caused by malfunctioning NTPase can lead to mitochondrial dysfunction contributing to disorders like mitochondrial myopathy. In cancer altered nucleotide metabolism may support uncontrolled cell proliferation. NTPase could connect with proteins such as p53 or oncogene products accumulating interest as a potential therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154), expandable thumbnail

    Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)

    Lanes 1-4: Merged signal (red and green). Green - ab182154 observed at 21 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab182154 Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] was shown to specifically react with Nucleoside triphosphate phosphohydrolase in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line Human NTPCR (Nucleoside triphosphate phosphohydrolase) knockout HEK-293T cell line ab266397 (knockout cell lysate Human NTPCR (Nucleoside triphosphate phosphohydrolase) knockout HEK-293T cell lysate ab258083) was used. Wild-type and Nucleoside triphosphate phosphohydrolase knockout samples were subjected to SDS-PAGE. ab182154 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

    Lane 2: NTPCR knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

    Lane 2: Western blot - Human NTPCR (Nucleoside triphosphate phosphohydrolase) knockout HEK-293T cell line (Human NTPCR (Nucleoside triphosphate phosphohydrolase) knockout HEK-293T cell line ab266397)

    Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

    Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Flow Cytometry (Intracellular) - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)

    Intracellular flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling Nucleoside triphosphate phosphohydrolase with ab182154 at 1/230 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154), expandable thumbnail

    Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)

    Western blot analysis of immunoprecipitation pellet from U87-MG cell lysate immunoprecipitated using ab182154 at 1/20 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    All lanes: Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)

    Predicted band size: 21 kDa

  • Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154), expandable thumbnail

    Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)

    All lanes: Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154) at 1/2000 dilution

    Lane 1: U87-MG cell lysate at 20 µg

    Lane 2: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 21 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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