AB182154

Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325]

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal Nucleoside triphosphate phosphohydrolase antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C1orf57, NTPCR, Cancer-related nucleoside-triphosphatase, NTPase, Nucleoside triphosphate phosphohydrolase

4 Images

Flow Cytometry (Intracellular) - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (AB182154)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (AB182154)

Intracellular flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling Nucleoside triphosphate phosphohydrolase with ab182154 at 1/230 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Supplier Data

Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (AB182154)

Western blot analysis of immunoprecipitation pellet from U87-MG cell lysate immunoprecipitated using ab182154 at 1/20 dilution.
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)

Predicted band size: 21 kDa

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Supplier Data

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (AB182154)

All lanes:

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154) at 1/2000 dilution

Lane 1:

U87-MG cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 21 kDa

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Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (AB182154)

Lanes 1-4 : Merged signal (red and green). Green - ab182154 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.

ab182154 Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] was shown to specifically react with Nucleoside triphosphate phosphohydrolase in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266397 (knockout cell lysate ab258083) was used. Wild-type and Nucleoside triphosphate phosphohydrolase knockout samples were subjected to SDS-PAGE. ab182154 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

NTPCR knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human NTPCR (Nucleoside triphosphate phosphohydrolase) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ntpcr-nucleoside-triphosphate-phosphohydrolase-knockout-hek-293t-cell-line-ab266397'>ab266397</a>)

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

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Carrier free

Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14325

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/230", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoside triphosphate phosphohydrolase often called NTPase is an enzyme responsible for the hydrolysis of nucleoside triphosphates. It plays an important role in converting nucleoside triphosphates to nucleoside diphosphates with the release of inorganic phosphate. This process is important as it regulates the availability of nucleotides for various biological functions. NTPase typically with a mass of approximately 70 kDa is expressed in various tissues but shows high activity in tissues with rapid cell division. It is particularly studied in cellular lines such as HEK293T where it assists in the investigation of cellular mechanisms.

Biological function summary

This enzyme participates in critical cellular processes by managing the concentration of nucleoside triphosphates. Its activity influences DNA and RNA synthesis signal transduction and energy transfer. NTPase does not generally form complexes with other proteins but may interact with other molecules transiently during its catalytic action. This transient nature allows it to act efficiently where nucleoside triphosphate function is necessary.

Pathways

NTPase influences multiple biochemical pathways important for cell viability and function. It plays a role in the nucleotide metabolism pathway which is essential for DNA and RNA biosynthesis. Moreover its involvement in the energy transfer pathway ties it to proteins such as myosin and kinases which require energy from ATP hydrolysis to function properly. These interactions underline its importance in cellular physiology.

Disruptions in NTPase activity have been linked to mitochondrial disorders and certain cancers. Abnormal levels of nucleoside triphosphates caused by malfunctioning NTPase can lead to mitochondrial dysfunction contributing to disorders like mitochondrial myopathy. In cancer altered nucleotide metabolism may support uncontrolled cell proliferation. NTPase could connect with proteins such as p53 or oncogene products accumulating interest as a potential therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has nucleotide phosphatase activity towards ATP, GTP, CTP, TTP and UTP. Hydrolyzes nucleoside diphosphates with lower efficiency.

See full target information NTPCR

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:61 PubMed34934432

2021

LIM mineralization protein-1 inhibits IL-1β-induced human chondrocytes injury by altering the NF-κB and MAPK/JNK pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Dijun Ou,Sheng Liu,Changjun Tong,Hezhong Tan,Yadong Yang,Chunlei He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325]

Flow Cytometry (Intracellular) - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (AB182154)

Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (AB182154)

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (AB182154)

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (AB182154)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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