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AB250596

Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free

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Rabbit Recombinant Monoclonal Nucleoside triphosphate phosphohydrolase antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

C1orf57, NTPCR, Cancer-related nucleoside-triphosphatase, NTPase, Nucleoside triphosphate phosphohydrolase

4 Images
Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (AB250596)
  • WB

Unknown

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (AB250596)

This data was developed using ab182154, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab182154 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.

ab182154 Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] was shown to specifically react with Nucleoside triphosphate phosphohydrolase in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266397 (knockout cell lysate ab258083) was used. Wild-type and Nucleoside triphosphate phosphohydrolase knockout samples were subjected to SDS-PAGE. ab182154 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (<a href='/en-us/products/primary-antibodies/nucleoside-triphosphate-phosphohydrolase-antibody-epr14325-ab182154'>ab182154</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

NTPCR knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human NTPCR (Nucleoside triphosphate phosphohydrolase) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ntpcr-nucleoside-triphosphate-phosphohydrolase-knockout-hek-293t-cell-line-ab266397'>ab266397</a>)

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (AB250596)
  • IP

Unknown

Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (AB250596)

This data was developed using ab182154, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from U87-MG cell lysate immunoprecipitated using ab182154 at 1/20 dilution.
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (ab250596)

Predicted band size: 21 kDa

false

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (AB250596)
  • WB

Supplier Data

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (AB250596)

This data was developed using ab182154, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (ab250596) at 1/2000 dilution

Lane 1:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 21 kDa

false

Flow Cytometry (Intracellular) - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (AB250596)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] - BSA and Azide free (AB250596)

This data was developed using ab182154, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling Nucleoside triphosphate phosphohydrolase with ab182154 at 1/230 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Unconjugated

    Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14325

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250596 is the carrier-free version of ab182154.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoside triphosphate phosphohydrolase often called NTPase is an enzyme responsible for the hydrolysis of nucleoside triphosphates. It plays an important role in converting nucleoside triphosphates to nucleoside diphosphates with the release of inorganic phosphate. This process is important as it regulates the availability of nucleotides for various biological functions. NTPase typically with a mass of approximately 70 kDa is expressed in various tissues but shows high activity in tissues with rapid cell division. It is particularly studied in cellular lines such as HEK293T where it assists in the investigation of cellular mechanisms.
Biological function summary

This enzyme participates in critical cellular processes by managing the concentration of nucleoside triphosphates. Its activity influences DNA and RNA synthesis signal transduction and energy transfer. NTPase does not generally form complexes with other proteins but may interact with other molecules transiently during its catalytic action. This transient nature allows it to act efficiently where nucleoside triphosphate function is necessary.

Pathways

NTPase influences multiple biochemical pathways important for cell viability and function. It plays a role in the nucleotide metabolism pathway which is essential for DNA and RNA biosynthesis. Moreover its involvement in the energy transfer pathway ties it to proteins such as myosin and kinases which require energy from ATP hydrolysis to function properly. These interactions underline its importance in cellular physiology.

Disruptions in NTPase activity have been linked to mitochondrial disorders and certain cancers. Abnormal levels of nucleoside triphosphates caused by malfunctioning NTPase can lead to mitochondrial dysfunction contributing to disorders like mitochondrial myopathy. In cancer altered nucleotide metabolism may support uncontrolled cell proliferation. NTPase could connect with proteins such as p53 or oncogene products accumulating interest as a potential therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has nucleotide phosphatase activity towards ATP, GTP, CTP, TTP and UTP. Hydrolyzes nucleoside diphosphates with lower efficiency.
See full target information NTPCR
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com