Anti-Nucleostemin antibody

7 product images

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  Western blot - Anti-Nucleostemin antibody (ab70346)

  Detection: Chemiluminescence with an exposure time of 10 seconds

  All lanes: Western blot - Anti-Nucleostemin antibody (ab70346) at 1/5000 dilution

  Lane 1: HeLa Whole cell lysate at 50 µg

  Lane 2: HEK293T Whole cell lysate at 50 µg

  Lane 3: Jurkat Whole cell lysate at 50 µg

  Secondary

  All lanes: Goat anti-Rabbit Light Chain HRP Conjugate

  Predicted band size: 62 kDa

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  Western blot - Anti-Nucleostemin antibody (ab70346)

  Detection: Chemiluminescence with an exposure time of 30 seconds.

  All lanes: Western blot - Anti-Nucleostemin antibody (ab70346) at 1/2000 dilution

  Lane 1: TMCK-1 Whole cell lysate at 50 µg

  Lane 2: NIH 3T3 :Whole cell lysate at 50 µg

  Secondary

  All lanes: Goat anti-Rabbit Light Chain HRP Conjugate

  Predicted band size: 62 kDa

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  Immunoprecipitation - Anti-Nucleostemin antibody (ab70346)

  Nucleostemin was immunoprecipitated from HEK-293T whole cell lysate with 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab70346 at 0.04 μg/ml dilution.
  

  Lane 1: ab70346 IP in HEK-293T whole cell lysate.

  Lane 2: Control IgG IP in HEK-293T whole cell lysate.

  All lanes: Immunoprecipitation - Anti-Nucleostemin antibody (ab70346)

  Predicted band size: 62 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)

  Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded section of mouse CT26 colon carcinoma tissue labelling Nucleostemin with ab70346 at 1:200 (1μg/ml). Detection: DAB.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)

  Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded section of human breast carcinoma tissue labelling Nucleostemin with ab70346 at 1:200 (1μg/ml). Detection: DAB.

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  Immunocytochemistry/ Immunofluorescence - Anti-Nucleostemin antibody (ab70346)

  ICC/IF image of ab70346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70346, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

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  This image is courtesy of a customer review submitted by Carl Hobbs, King's College London, United Kingdom

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)

  Immunohistochemistical detection of Nucleostemin with ab70346 on formaldehyde-fixed paraffin-embedded human colon sections. Antigen retrieval step: heat mediated in citric acid pH6 buffer. Blocking step: 1% BSA for 10 mins @ rt°C. Primary antibody ab70346 incubated at 1/50 for 16 hours in TBS/BSA/azide. Secondary antibody: anti-rabbit IgG conjugated to biotin (1/200). The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. Stem cell markers have been observed in cycling columnar cells at the crypt base (Nature 449, 1003-1007; 2007). The cells (nuclei of the simple columnar epithelium of the upper mucosa) immunoreactive for Nucleostemin in this image (green arrowheads) anatomically correspond to the same cells expected to be Lgr5 positivite. A red arrowhead indicates nucleolar positivity in one of many cell nuclei in the Lamina propria of the mucosa (this area is not known for stem cell posit