Skip to main content

Rabbit Recombinant Monoclonal Nucleostemin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (AB248337), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (AB248337), expandable thumbnail
  • OI-RD Scanning - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (AB248337), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Tested

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Nucleostemin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR3911(2)

Purification technique

Affinity purification

Dissociation constant

6.76 x 10-11 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248337 is the carrier-free version of Anti-Nucleostemin antibody [EPR3911(2)] ab129185.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Nucleostemin is a protein involved in cellular regulation and has an approximate mass of 60 kDa. It is also known by the alternative name GNL3. Mechanical function of nucleostemin involves involvement in the regulation of cell cycle and maintenance of stem cell properties. This protein is expressed mainly in the nucleolus and is found in abundance within stem cells and certain cancer cells where its levels change depending on cell cycle stages.

Biological function summary

Nucleostemin plays an important role in controlling cell proliferation and maintaining stem cell characteristics. It is not part of a large complex but interacts with other proteins to perform its functions. The protein facilitates the regulation of cell division and its presence in stem cells assures the maintenance of an undifferentiated state which is important for tissue regeneration and repair.

Pathways

Nucleostemin interacts with various cell cycle and DNA repair mechanisms. Among important biological pathways nucleostemin is involved in the p53 signaling pathway assisting in cellular stress responses and enabling cell survival during DNA damage. There is also a relationship with the MDM2 protein as nucleostemin indirectly influences p53 stability by modulating MDM2 activity therefore playing a role in tumor suppression and oncogenic processes.

Associated diseases and disorders

Researchers associate nucleostemin with cancer and neurological disorders. In cancers its overexpression is linked to the unchecked proliferation of tumor cells often observed in glioblastoma and certain sarcomas where nucleostemin interacts with proteins like p53 and MDM2 to influence tumor dynamics. In neurological contexts its role in stem cell regulation draws attention when considering diseases involving neurodegeneration where maintaining neural stem cells remains important.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (ab248337), expandable thumbnail

    Western blot - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (ab248337)

    This data was developed using Anti-Nucleostemin antibody [EPR3911(2)] ab129185, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Nucleostemin antibody [EPR3911(2)] (Anti-Nucleostemin antibody [EPR3911(2)] ab129185) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: PC-3 cell lysate at 10 µg

    Lane 3: SW480 cell lysate at 10 µg

    Lane 4: K562 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 62 kDa

  • Flow Cytometry (Intracellular) - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (ab248337), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (ab248337)

    This data was developed using Anti-Nucleostemin antibody [EPR3911(2)] ab129185, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of permeabilized SW480 cells using Anti-Nucleostemin antibody [EPR3911(2)] ab129185 (red) at 1/10 dilution or a rabbit IgG (negative) (green).

  • OI-RD Scanning - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (ab248337), expandable thumbnail

    OI-RD Scanning - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (ab248337)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com