Rabbit Recombinant Monoclonal Nucleostemin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IP | WB | IHC-P | Flow Cyt (Intra) | |
---|---|---|---|---|
Human | Not recommended | Tested | Not recommended | Tested |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).
E2IG3, NS, GNL3, Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, Novel nucleolar protein 47, Nucleolar GTP-binding protein 3, Nucleostemin, NNP47
Rabbit Recombinant Monoclonal Nucleostemin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR3911(2)
Affinity purification
6.76 x 10-11 M
Blue Ice
+4°C
+4°C
Do Not Freeze
ab248337 is the carrier-free version of Anti-Nucleostemin antibody [EPR3911(2)] ab129185.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Nucleostemin is a protein involved in cellular regulation and has an approximate mass of 60 kDa. It is also known by the alternative name GNL3. Mechanical function of nucleostemin involves involvement in the regulation of cell cycle and maintenance of stem cell properties. This protein is expressed mainly in the nucleolus and is found in abundance within stem cells and certain cancer cells where its levels change depending on cell cycle stages.
Nucleostemin plays an important role in controlling cell proliferation and maintaining stem cell characteristics. It is not part of a large complex but interacts with other proteins to perform its functions. The protein facilitates the regulation of cell division and its presence in stem cells assures the maintenance of an undifferentiated state which is important for tissue regeneration and repair.
Nucleostemin interacts with various cell cycle and DNA repair mechanisms. Among important biological pathways nucleostemin is involved in the p53 signaling pathway assisting in cellular stress responses and enabling cell survival during DNA damage. There is also a relationship with the MDM2 protein as nucleostemin indirectly influences p53 stability by modulating MDM2 activity therefore playing a role in tumor suppression and oncogenic processes.
Researchers associate nucleostemin with cancer and neurological disorders. In cancers its overexpression is linked to the unchecked proliferation of tumor cells often observed in glioblastoma and certain sarcomas where nucleostemin interacts with proteins like p53 and MDM2 to influence tumor dynamics. In neurological contexts its role in stem cell regulation draws attention when considering diseases involving neurodegeneration where maintaining neural stem cells remains important.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
This data was developed using Anti-Nucleostemin antibody [EPR3911(2)] ab129185, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Nucleostemin antibody [EPR3911(2)] (Anti-Nucleostemin antibody [EPR3911(2)] ab129185) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: PC-3 cell lysate at 10 µg
Lane 3: SW480 cell lysate at 10 µg
Lane 4: K562 cell lysate at 10 µg
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 62 kDa
This data was developed using Anti-Nucleostemin antibody [EPR3911(2)] ab129185, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized SW480 cells using Anti-Nucleostemin antibody [EPR3911(2)] ab129185 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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