Rabbit Recombinant Monoclonal NUDCD2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (AB249691), expandable thumbnail](https://content.abcam.com/products/images/nudcd2-antibody-epr11558-bsa-and-azide-free-ab249691--western-blot-img105388.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (AB249691)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (AB249691), expandable thumbnail](https://content.abcam.com/products/images/nudcd2-antibody-epr11558-bsa-and-azide-free-ab249691--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113669.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (AB249691)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (AB249691), expandable thumbnail](https://content.abcam.com/products/images/nudcd2-antibody-epr11558-bsa-and-azide-free-ab249691--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56786.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (AB249691)")
![Flow Cytometry (Intracellular) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (AB249691), expandable thumbnail](https://content.abcam.com/products/images/nudcd2-antibody-epr11558-bsa-and-azide-free-ab249691--flow-cytometry-intracellular-img79744.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (AB249691)")
![Immunoprecipitation - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (AB249691), expandable thumbnail](https://content.abcam.com/products/images/nudcd2-antibody-epr11558-bsa-and-azide-free-ab249691--immunoprecipitation-img69122.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (AB249691)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
May regulate the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone.
Alternative names
NudC domain-containing protein 2, NUDCD2
Recommended products
Rabbit Recombinant Monoclonal NUDCD2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Alternative names
NudC domain-containing protein 2, NUDCD2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR11558
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab249691 is the carrier-free version of Anti-NUDCD2 antibody [EPR11558] ab171979.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NUDCD2 also known as NudC Domain Containing 2 is a protein that plays a role in cellular processes like protein transport. This protein weighs approximately 45 kDa. NUDCD2 locates primarily in the cytoplasm where it interacts with microtubules. The expression profile of NUDCD2 shows its presence in multiple tissues including liver lungs and lymph nodes highlighting its significance in these areas.
- Biological function summary
The actions of NUDCD2 involve interactions with the mitotic spindle suggesting a role in cell division. NUDCD2 associates with elements of the dynein-dynactin complex which implies participation in intracellular transport mechanisms. This complex facilitates processes essential for efficient mitotic spindle function an important aspect of cell division and proper chromosome segregation.
- Pathways
NUDCD2 is involved in the regulation of the microtubule cytoskeleton. It functions within pathways like the cell cycle regulation pathway in particular during mitosis. NUDCD2 is related to proteins such as dynein which contribute to the stabilization of microtubules and proper mitotic spindle formation. This connection indicates that NUDCD2 impacts processes that control cell proliferation and genomic stability.
- Associated diseases and disorders
NUDCD2 has connections to cancer where disruptions in its function could lead to abnormal cell division and tumor formation. Impaired regulation of proteins like NUDCD2 might result in missegregation of chromosomes promoting oncogenesis. Additionally NUDCD2 implications in neurological disorders exist since defects in microtubule-related processes can lead to neurodevelopmental issues linking it with proteins involved in neuronal function and stability.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691), expandable thumbnail](https://content.abcam.com/products/images/nudcd2-antibody-epr11558-bsa-and-azide-free-ab249691--western-blot-img105388.jpg "Western blot - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691)")
Western blot - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691)
This data was developed using Anti-NUDCD2 antibody [EPR11558] ab171979, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-NUDCD2 antibody [EPR11558] (Anti-NUDCD2 antibody [EPR11558] ab171979) at 1/1000 dilution
Lane 1: K562 cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Predicted band size: 18 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691), expandable thumbnail](https://content.abcam.com/products/images/nudcd2-antibody-epr11558-bsa-and-azide-free-ab249691--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113669.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691)
This data was developed using Anti-NUDCD2 antibody [EPR11558] ab171979, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling NUDCD2 with Anti-NUDCD2 antibody [EPR11558] ab171979 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691), expandable thumbnail](https://content.abcam.com/products/images/nudcd2-antibody-epr11558-bsa-and-azide-free-ab249691--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56786.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691)
This data was developed using Anti-NUDCD2 antibody [EPR11558] ab171979, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling NUDCD2 with Anti-NUDCD2 antibody [EPR11558] ab171979 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691), expandable thumbnail](https://content.abcam.com/products/images/nudcd2-antibody-epr11558-bsa-and-azide-free-ab249691--flow-cytometry-intracellular-img79744.jpg "Flow Cytometry (Intracellular) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691)")
Flow Cytometry (Intracellular) - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691)
This data was developed using Anti-NUDCD2 antibody [EPR11558] ab171979, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized 293T cells labeling NUDCD2 with Anti-NUDCD2 antibody [EPR11558] ab171979 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).
![Immunoprecipitation - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691), expandable thumbnail](https://content.abcam.com/products/images/nudcd2-antibody-epr11558-bsa-and-azide-free-ab249691--immunoprecipitation-img69122.jpg "Immunoprecipitation - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691)")
Immunoprecipitation - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691)
This data was developed using Anti-NUDCD2 antibody [EPR11558] ab171979, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from 293T cell lysate labeling NUDCD2 with Anti-NUDCD2 antibody [EPR11558] ab171979 at 1/10 dilution.
All lanes: Immunoprecipitation - Anti-NUDCD2 antibody [EPR11558] (Anti-NUDCD2 antibody [EPR11558] ab171979)
Predicted band size: 18 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691), expandable thumbnail](https://content.abcam.com/products/images/nudcd2-antibody-epr11558-bsa-and-azide-free-ab249691--immunocytochemistry-immunofluorescence-img14917.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691)")
Immunocytochemistry/ Immunofluorescence - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691)
This data was developed using Anti-NUDCD2 antibody [EPR11558] ab171979, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labeling NUDCD2 with Anti-NUDCD2 antibody [EPR11558] ab171979 at 1/100 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691), expandable thumbnail](https://content.abcam.com/products/images/nudcd2-antibody-epr11558-bsa-and-azide-free-ab249691--immunocytochemistry-immunofluorescence-img14917.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NUDCD2 antibody [EPR11558] - BSA and Azide free (ab249691)")
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