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AB197310

Anti-NUDT12 antibody

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(2 Publications)

Rabbit Polyclonal NUDT12 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUDT12 aa 150-400.

View Alternative Names

NAD-capped RNA hydrolase NUDT12, DeNADding enzyme NUDT12, NADH pyrophosphatase NUDT12, Nucleoside diphosphate-linked moiety X motif 12, Nudix motif 12, NUDT12

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT12 antibody (AB197310)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT12 antibody (AB197310)

Immunohistochemical analysis of formaliln-fixed, paraffin-embedded Human fetal stomach tissue labeling NUDT12 with ab197310 at 1/100 dilution.

Western blot - Anti-NUDT12 antibody (AB197310)
  • WB

Supplier Data

Western blot - Anti-NUDT12 antibody (AB197310)

All lanes:

Western blot - Anti-NUDT12 antibody (ab197310) at 1/500 dilution

Lane 1:

293T cell lysate

Lane 2:

HepG2 cell lysate

Lane 3:

HeLa cell lysate

Lane 4:

A549 cell lysate

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NUDT12 aa 150-400. The exact immunogen used to generate this antibody is proprietary information.

Q9BQG2

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Reactivity data

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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUDT12 also known as Nudix hydrolase 12 plays a role in hydrolyzing nucleoside diphosphates. It mainly acts on ADP-ribose a product from NAD+ metabolism. The molecular mass of NUDT12 is approximately 43 kDa. Expression of NUDT12 occurs significantly in liver tissues and to some extent in other tissues as well. It contributes to maintaining nucleotide pools ensuring cellular homeostasis by freeing the cells from non-canonical nucleotides that can be harmful.
Biological function summary

NUDT12 regulates cellular functions by processing NAD+ metabolites. It forms part of a complex involved with enzymes that manage nucleotide hydrolysis. By removing non-standard nucleotides from the internal environment it prevents harmful incorporation into nucleic acids. Its activity supports processes like DNA repair safeguarding genome integrity which is important for normal cell function.

Pathways

NUDT12 functions within NAD+ metabolism and the nucleotide salvage pathway. It is part of a group of Nudix hydrolases that includes proteins like NUDT1. These proteins collectively aid in nucleotide management and energy homeostasis. By collaborating in these pathways NUDT12 ensures metabolic processes proceed without interference from aberrant molecules.

Dysfunction in NUDT12 is linked to conditions related to metabolic imbalance such as metabolic syndrome and certain liver diseases. This is due to its role in managing nucleotide pools a process implicated in the pathology of these disorders. Connections between NUDT12 and proteins like PARP which is involved in DNA repair and epigenetic regulation highlight its potential impact on disease mechanisms where NAD+ and its derivatives play roles.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

mRNA decapping enzyme that specifically removes the nicotinamide adenine dinucleotide (NAD) cap from a subset of mRNAs by hydrolyzing the diphosphate linkage to produce nicotinamide mononucleotide (NMN) and 5' monophosphate mRNA (PubMed : 31101919, PubMed : 31875550). The NAD-cap is present at the 5'-end of some RNAs; in contrast to the canonical N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay (PubMed : 31101919). Preferentially acts on NAD-capped transcripts in response to nutrient stress (PubMed : 31101919). Also acts on free nicotinamide adenine dinucleotide molecules : hydrolyzes NAD(H) into NMN(H) and AMP, and NADPH into NMNH and 2',5'-ADP (PubMed : 12790796). May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle (PubMed : 12790796). Regulates the levels of circadian clock components PER1, PER2, PER3 and CRY2 in the liver (By similarity).
See full target information NUDT12
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 23:292 PubMed37004045

2023

Aspartate beta-hydroxylase domain containing 1 as a prognostic marker associated with immune infiltration in skin cutaneous melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shiquan Sun,Min Deng,Juan Wen,Xiaoyuan Chen,Jiaqi Xu,Yu Liu,Huanhuan Wan,Jin Wang,Leping Yan,Yong He,Yunsheng Xu

Nature chemical biology 15:575-582 PubMed31101919

2019

Structural and mechanistic basis of mammalian Nudt12 RNA deNADding.

Applications

Unspecified application

Species

Unspecified reactive species

Ewa Grudzien-Nogalska,Yixuan Wu,Xinfu Jiao,Huijuan Cui,Maria K Mateyak,Ronald P Hart,Liang Tong,Megerditch Kiledjian
View all publications
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Product promise

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