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AB204739

Anti-NUDT16 antibody

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(1 Publication)

Rabbit Polyclonal NUDT16 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NUDT16.

View Alternative Names

U8 snoRNA-decapping enzyme, IDP phosphatase, Inosine diphosphate phosphatase, Nucleoside diphosphate-linked moiety X motif 16, Nudix hydrolase 16, U8 snoRNA-binding protein H29K, m7GpppN-mRNA hydrolase, IDPase, Nudix motif 16, NUDT16

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-NUDT16 antibody (AB204739)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUDT16 antibody (AB204739)

Immunofluorescent analysis of MCF7 cells labeling NUDT16 with ab204739 at 4 μg/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT16 antibody (AB204739)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT16 antibody (AB204739)

Immunohistochemical analysis of paraffin-embedded Human appendix tissue labeling NUDT16 with ab204739 at a dilution of 1/20.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NUDT16. The exact immunogen used to generate this antibody is proprietary information.

Q96DE0

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUDT16 also known as diphosphatase is an enzyme that belongs to the nudix hydrolase family. It has a molecular mass of approximately 19 kDa. This enzyme cleaves diphosphate groups from nucleoside diphosphate derivatives. NUDT16 is highly expressed in several tissues including liver and testis. Its activity is important for maintaining cellular nucleotide pool balance by removing non-canonical nucleotides that can potentially be harmful if incorporated into DNA or RNA.
Biological function summary

NUDT16 functions to regulate nucleotide metabolism. It catalyzes the hydrolysis of nucleoside diphosphates like diadenosine tetraphosphate into monophosphates. NUDT16 often operates with other proteins in multi-enzyme complexes where it plays a role in modulating the concentrations of nucleoside diphosphates within cells. This activity coordinates the synthesis and degradation of nucleotides influencing cellular energy status and coenzyme levels.

Pathways

With regards to pathways NUDT16 integrates into nucleotide catabolism and salvage pathways. These pathways facilitate the conversion and recycling of nucleotides ensuring a steady supply for nucleic acid synthesis. NUDT16 interacts within these pathways alongside other key enzymes such as IMP dehydrogenase and GMP synthetase highlighting its role in controlling guanine nucleotide levels. This involvement provides a direct link to DNA replication and repair mechanisms which depend on the accurate control of nucleotide pools.

NUDT16 has connections to cancer and autoimmune diseases. Abnormal activity or expression of NUDT16 may contribute to the accumulation of atypical nucleotides potentially leading to mutations and genomic instability that characterize several cancer types. In autoimmune contexts altered NUDT16 function can impact immune cell activation and response. It exhibits an intriguing relationship with proteins like SAMHD1 a known regulator of nucleotide pools involved in immune responses and cancer.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Also catalyzes the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Also acts as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms (PubMed : 20385596, PubMed : 26121039). The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP (PubMed : 20385596). Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions (PubMed : 20385596). Exhibits decapping activity towards NAD-capped RNAs and FAD-capped RNAs (PubMed : 32432673). Exhibits decapping activity towards dpCoA-capped RNAs in vitro (By similarity).
See full target information NUDT16
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Renal failure 47:2536729 PubMed40775604

2025

Identification of novel pathogenic mutations in associated with distal renal tubular acidosis and analysis of wild-type expression in glomerular disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yaru Jiang,Menglu Ren,Chun Gan,Qing Yang,Huimin Jiang,Han Xiao,Xindi Zhou,Wanbing Chen,Xuejun Yang,Baohui Yang,Yaxi Chen,Mo Wang,Haiping Yang,Wei Jiang,Qiu Li
View all publications
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