AB180166

Anti-NUDT19 antibody [EPR13162-63]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal NUDT19 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Acyl-coenzyme A diphosphatase NUDT19, Nucleoside diphosphate-linked moiety X motif 19, Nudix motif 19, NUDT19

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT19 antibody [EPR13162-63] (AB180166)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT19 antibody [EPR13162-63] (AB180166)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling NUDT19 using ab180166 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-NUDT19 antibody [EPR13162-63] (AB180166)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUDT19 antibody [EPR13162-63] (AB180166)

Immunofluorescence analysis of 293T cells labeling NUDT19 using ab180166 at 1/100 (green). DAPI nuclear staining (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT19 antibody [EPR13162-63] (AB180166)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NUDT19 using ab180166 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-NUDT19 antibody [EPR13162-63] (AB180166)

All lanes:

Western blot - Anti-NUDT19 antibody [EPR13162-63] (ab180166) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Human ovary tissue lysate at 10 µg

Lane 4:

Human spleen tissue lysate at 10 µg

Predicted band size: 42 kDa

true

Carrier free

Anti-NUDT19 antibody [EPR13162-63] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13162-63

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUDT19 also known as Diphosphatase NUDT19 is a protein that functions by hydrolyzing nucleoside diphosphates which are involved in various biochemical processes such as cell signaling and metabolism. It carries a molecular mass of approximately 22 kDa. NUDT19 mostly expresses in the kidneys and liver playing important roles in detoxification and energy metabolism in these tissues.

Biological function summary

NUDT19 is involved in regulating intracellular levels of Coenzyme A and its derivatives through its enzymatic action. By breaking down these molecules it helps maintain cellular metabolic balance. While it does not function as part of a larger complex its activity directly impacts the availability of metabolic substrates required for energy production and other cellular functions.

Pathways

NUDT19 plays a critical role in the Coenzyme A biosynthesis pathway. It contributes to the regulation of this pathway by controlling the levels of intermediates through its enzymatic activity. This protein links to other key enzymes such as ACSM3 which plays a part in the catabolism of fatty acids. These interactions highlight NUDT19’s involvement in lipid metabolism and energy balance.

NUDT19 has connections to metabolic disorders such as diabetes and fatty liver disease. Abnormal activity or expression of NUDT19 may lead to disruptions in energy metabolism contributing to the pathology of these conditions. Its role in managing Coenzyme A derivatives also ties it to SIRT5 a protein implicated in metabolic regulation and stress responses. Understanding NUDT19’s function could lead to insights into therapeutic strategies for related metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate (By similarity). Mediates the hydrolysis of a wide range of CoA esters, including choloyl-CoA and branched-chain fatty-acyl-CoA esters and at low substrate concentrations medium and long-chain fatty-acyl-CoA esters are the primary substrates (By similarity). Highest activity seen with medium-chain acyl-CoA esters and higher rates of activity seen with the unsaturated acyl-CoA esters compared with the saturated esters (By similarity). Exhibits decapping activity towards dpCoA-capped RNAs in vitro (By similarity).

See full target information Nucleoside diphosphate-linked moiety X motif 19

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Medical science monitor : international medical jo 22:2357-62 PubMed27385084

2016

Thymic Stromal Lymphopoietin Promotes Fibrosis and Activates Mitogen-Activated Protein Kinases in MRC-5 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Su Tang,Xiaodong Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUDT19 antibody [EPR13162-63]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT19 antibody [EPR13162-63] (AB180166)

Immunocytochemistry/ Immunofluorescence - Anti-NUDT19 antibody [EPR13162-63] (AB180166)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT19 antibody [EPR13162-63] (AB180166)

Western blot - Anti-NUDT19 antibody [EPR13162-63] (AB180166)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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