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AB221994

Anti-NUDT21/CFIM25 antibody

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(1 Publication)

Rabbit Polyclonal NUDT21/CFIM25 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NUDT21 aa 1-150.

View Alternative Names

CFIM25, CPSF25, CPSF5, NUDT21, Cleavage and polyadenylation specificity factor subunit 5, Cleavage and polyadenylation specificity factor 25 kDa subunit, Cleavage factor Im complex 25 kDa subunit, Nucleoside diphosphate-linked moiety X motif 21, Nudix hydrolase 21, Pre-mRNA cleavage factor Im 68 kDa subunit, CPSF 25 kDa subunit, CFIm25, Nudix motif 21

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-NUDT21/CFIM25 antibody (AB221994)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUDT21/CFIM25 antibody (AB221994)

PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma cell line) cells stained for NUDT21/CFIM25 (green) using ab221994 at 4 μg/ml in ICC/IF.

Western blot - Anti-NUDT21/CFIM25 antibody (AB221994)
  • WB

Supplier Data

Western blot - Anti-NUDT21/CFIM25 antibody (AB221994)

All lanes:

Western blot - Anti-NUDT21/CFIM25 antibody (ab221994) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NUDT21 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O43809

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUDT21 also known as CFIm25 is a protein important for RNA processing. This protein is part of the cleavage factor Im complex and plays an important role in pre-mRNA polyadenylation. NUDT21 is about 25 kDa in molecular weight and expresses widely across human tissues. It is especially abundant in the brain and liver indicating important roles in these areas.
Biological function summary

NUDT21 acts as a component of the cleavage factor I which is essential for mRNA polyadenylation. This protein ensures proper 3’-end processing of pre-mRNA by interacting with other CFIm complex components like CPSF and CstF. This interaction is necessary for the regulation of mRNA stability and eventual translation into proteins. The correct functioning of NUDT21 is necessary for the maintenance of gene expression.

Pathways

NUDT21 is part of the mRNA 3'-end processing pathway. This pathway is critical for proper gene expression affecting mRNA synthesis and degradation rates. Within this pathway NUDT21 works closely with proteins such as CPSF6 and CPSF7 facilitating the assembly of the mRNA cleavage complex. The pathway influences general RNA growth and stability impacting numerous cellular processes.

Abnormalities in NUDT21 are linked to neurological disorders and cancer. Loss of function or altered expression is connected to intellectual disability affecting neural development and cognitive abilities. In the context of cancer NUDT21 levels can influence tumor growth and progression. Changes in NUDT21 may cause dysregulation in associated proteins like CPSF6 further complicating these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed : 14690600, PubMed : 15937220, PubMed : 17024186, PubMed : 17098938, PubMed : 29276085, PubMed : 8626397, PubMed : 9659921). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed : 14690600, PubMed : 17024186, PubMed : 8626397, PubMed : 9659921). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed : 17098938, PubMed : 23187700, PubMed : 29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed : 17098938, PubMed : 20695905, PubMed : 29276085). NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7 (PubMed : 29276085). Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing (PubMed : 14690600, PubMed : 15169763, PubMed : 17024186, PubMed : 20479262, PubMed : 22813749, PubMed : 8626397). The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA (PubMed : 20479262, PubMed : 21295486). Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function (By similarity). Binds to chromatin (By similarity). Binds to, but does not hydrolyze mono- and di-adenosine nucleotides (PubMed : 18445629).
See full target information NUDT21
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Diabetology & metabolic syndrome 13:152 PubMed34963484

2021

MiR-423-5p activated by E2F1 promotes neovascularization in diabetic retinopathy by targeting HIPK2.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Xiao,Yinu Zhao,Hongjing Sun,Jia Xu,Wenjie Li,Limo Gao
View all publications
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