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Rabbit Recombinant Monoclonal NUDT5 antibody. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-NUDT5 antibody [EPR7735] (AB129163), expandable thumbnail
  • Western blot - Anti-NUDT5 antibody [EPR7735] (AB129163), expandable thumbnail
  • OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] (AB129163), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate (PubMed:27257257). In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP (PubMed:10567213, PubMed:10722730, PubMed:17052728, PubMed:19699693, PubMed:21389046). Can also hydrolyze other nucleotide sugars with low activity (PubMed:19699693, PubMed:21389046). In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate. Nuclear ATP synthesis takes place when dephosphorylated at Thr-45 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). Does not play a role in U8 snoRNA decapping activity (By similarity). Binds U8 snoRNA (By similarity).

Alternative names

NUDIX5, HSPC115, NUDT5, ADP-sugar pyrophosphatase, 8-oxo-dGDP phosphatase, Nuclear ATP-synthesis protein NUDIX5, Nucleoside diphosphate-linked moiety X motif 5, YSA1H, Nudix motif 5, hNUDT5

Recommended products

Rabbit Recombinant Monoclonal NUDT5 antibody. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR7735
Purification technique
Affinity purification Protein A
Dissociation constant
3.48 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Nudix hydrolase 5 (NUDT5) enzyme also known as ADP-sugar pyrophosphatase performs important mechanical roles by hydrolyzing ADP-ribose to ribose-5-phosphate and AMP. It holds a molecular mass of approximately 39 kDa. NUDT5 shows high expression levels in tissues like liver and ovary. Its activity involves detoxifying nucleoside diphosphate derivatives which prevents their accumulation and potential interference with normal cellular processes.

Biological function summary

NUDT5 influences intracellular signaling pathways and DNA repair processes. It operates as part of multi-enzyme complexes that manage nucleotide metabolism and signaling. By maintaining a balance of ADP-ribose inside cells NUDT5 helps regulate cellular stress responses and genomic stability. Its role in transforming ADP-ribose supports maintaining adequate cellular energy and nucleotide pools for various biological functions.

Pathways

Research identifies NUDT5 as being involved in the ADP-ribose metabolism pathway and DNA repair mechanisms. In the ADP-ribose pathway NUDT5 assists in the regulation of ADP-ribose levels working alongside other proteins like PARP1 which adds ADP-ribose units to proteins during DNA repair. NUDT5's actions in these pathways ensure the proper functioning of cellular stress responses and the prevention of genome instability.

Associated diseases and disorders

NUDT5 has connections to tumorigenesis and neurodegenerative disorders. Elevated activity of NUDT5 is sometimes observed in certain cancers suggesting a potential role in cancer cell viability. In neurodegenerative diseases such as Parkinson's dysfunctional ADP-ribose metabolism implicates NUDT5. Changes in NUDT5 activity can interact with proteins like PARP1 altering cellular processes that contribute to disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163), expandable thumbnail

    Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)

    Lanes 1-4: Merged signal (red and green). Green - ab129163 observed at 30 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab129163 Anti-NUDT5 antibody [EPR7735] was shown to specifically react with NUDT5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human NUDT5 knockout HEK-293T cell line ab266242 (knockout cell lysate Human NUDT5 knockout HEK-293T cell lysate ab258087) was used. Wild-type and NUDT5 knockout samples were subjected to SDS-PAGE. ab129163 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: NUDT5 knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human NUDT5 knockout HEK-293T cell line (Human NUDT5 knockout HEK-293T cell line ab266242)

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 24 kDa

    Observed band size: 30 kDa

  • Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163), expandable thumbnail

    Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)

    All lanes: Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: 293T cell lysate at 10 µg

    Lane 3: HepG2 cell lysate at 10 µg

    Lane 4: U937 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 24 kDa

    Observed band size: 33 kDa

  • OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] (ab129163), expandable thumbnail

    OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] (ab129163)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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