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AB129163

Anti-NUDT5 antibody [EPR7735]

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(1 Publication)

Rabbit Recombinant Monoclonal NUDT5 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NUDIX5, HSPC115, NUDT5, ADP-sugar pyrophosphatase, 8-oxo-dGDP phosphatase, Nuclear ATP-synthesis protein NUDIX5, Nucleoside diphosphate-linked moiety X motif 5, YSA1H, Nudix motif 5, hNUDT5

3 Images
Western blot - Anti-NUDT5 antibody [EPR7735] (AB129163)
  • WB

Lab

Western blot - Anti-NUDT5 antibody [EPR7735] (AB129163)

Lanes 1-4 : Merged signal (red and green). Green - ab129163 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.

ab129163 Anti-NUDT5 antibody [EPR7735] was shown to specifically react with NUDT5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266242 (knockout cell lysate ab258087) was used. Wild-type and NUDT5 knockout samples were subjected to SDS-PAGE. ab129163 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

NUDT5 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human NUDT5 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-nudt5-knockout-hek-293t-cell-line-ab266242'>ab266242</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 24 kDa

Observed band size: 30 kDa

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Western blot - Anti-NUDT5 antibody [EPR7735] (AB129163)
  • WB

Unknown

Western blot - Anti-NUDT5 antibody [EPR7735] (AB129163)

All lanes:

Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

U937 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 24 kDa

Observed band size: 33 kDa

false

OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] (AB129163)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] (AB129163)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-NUDT5 antibody [EPR7735] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7735

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Nudix hydrolase 5 (NUDT5) enzyme also known as ADP-sugar pyrophosphatase performs important mechanical roles by hydrolyzing ADP-ribose to ribose-5-phosphate and AMP. It holds a molecular mass of approximately 39 kDa. NUDT5 shows high expression levels in tissues like liver and ovary. Its activity involves detoxifying nucleoside diphosphate derivatives which prevents their accumulation and potential interference with normal cellular processes.
Biological function summary

NUDT5 influences intracellular signaling pathways and DNA repair processes. It operates as part of multi-enzyme complexes that manage nucleotide metabolism and signaling. By maintaining a balance of ADP-ribose inside cells NUDT5 helps regulate cellular stress responses and genomic stability. Its role in transforming ADP-ribose supports maintaining adequate cellular energy and nucleotide pools for various biological functions.

Pathways

Research identifies NUDT5 as being involved in the ADP-ribose metabolism pathway and DNA repair mechanisms. In the ADP-ribose pathway NUDT5 assists in the regulation of ADP-ribose levels working alongside other proteins like PARP1 which adds ADP-ribose units to proteins during DNA repair. NUDT5's actions in these pathways ensure the proper functioning of cellular stress responses and the prevention of genome instability.

NUDT5 has connections to tumorigenesis and neurodegenerative disorders. Elevated activity of NUDT5 is sometimes observed in certain cancers suggesting a potential role in cancer cell viability. In neurodegenerative diseases such as Parkinson's dysfunctional ADP-ribose metabolism implicates NUDT5. Changes in NUDT5 activity can interact with proteins like PARP1 altering cellular processes that contribute to disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate (PubMed : 27257257). In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP (PubMed : 10567213, PubMed : 10722730, PubMed : 17052728, PubMed : 19699693, PubMed : 21389046). Can also hydrolyze other nucleotide sugars with low activity (PubMed : 19699693, PubMed : 21389046). In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate. Nuclear ATP synthesis takes place when dephosphorylated at Thr-45 (PubMed : 27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed : 27257257). In vitro, it has decapping activity on 11-mer RNA capped with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) by hydrolyzing the pyrophosphate bridge of the sugar cap (PubMed : 38716860). Does not play a role in U8 snoRNA decapping (By similarity). Binds U8 snoRNA (By similarity).
See full target information NUDT5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of human genetics 107:311-324 PubMed32738225

2020

De Novo and Bi-allelic Pathogenic Variants in NARS1 Cause Neurodevelopmental Delay Due to Toxic Gain-of-Function and Partial Loss-of-Function Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Andreea Manole,Stephanie Efthymiou,Emer O'Connor,Marisa I Mendes,Matthew Jennings,Reza Maroofian,Indran Davagnanam,Kshitij Mankad,Maria Rodriguez Lopez,Vincenzo Salpietro,Ricardo Harripaul,Lauren Badalato,Jagdeep Walia,Christopher S Francklyn,Alkyoni Athanasiou-Fragkouli,Roisin Sullivan,Sonal Desai,Kristin Baranano,Faisal Zafar,Nuzhat Rana,Muhammed Ilyas,Alejandro Horga,Majdi Kara,Francesca Mattioli,Alice Goldenberg,Helen Griffin,Amelie Piton,Lindsay B Henderson,Benyekhlef Kara,Ayca Dilruba Aslanger,Joost Raaphorst,Rolph Pfundt,Ruben Portier,Marwan Shinawi,Amelia Kirby,Katherine M Christensen,Lu Wang,Rasim O Rosti,Sohail A Paracha,Muhammad T Sarwar,Dagan Jenkins,Jawad Ahmed,Federico A Santoni,Emmanuelle Ranza,Justyna Iwaszkiewicz,Cheryl Cytrynbaum,Rosanna Weksberg,Ingrid M Wentzensen,Maria J Guillen Sacoto,Yue Si,Aida Telegrafi,Marisa V Andrews,Dustin Baldridge,Heinz Gabriel,Julia Mohr,Barbara Oehl-Jaschkowitz,Sylvain Debard,Bruno Senger,Frédéric Fischer,Conny van Ravenwaaij,Annemarie J M Fock,Servi J C Stevens,Jürg Bähler,Amina Nasar,John F Mantovani,Adnan Manzur,Anna Sarkozy,Desirée E C Smith,Gajja S Salomons,Zubair M Ahmed,Shaikh Riazuddin,Saima Riazuddin,Muhammad A Usmani,Annette Seibt,Muhammad Ansar,Stylianos E Antonarakis,John B Vincent,Muhammad Ayub,Mona Grimmel,Anne Marie Jelsig,Tina Duelund Hjortshøj,Helena Gásdal Karstensen,Marybeth Hummel,Tobias B Haack,Yalda Jamshidi,Felix Distelmaier,Rita Horvath,Joseph G Gleeson,Hubert Becker,Jean-Louis Mandel,David A Koolen,Henry Houlden
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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