Anti-NUDT5 antibody [EPR7735] - BSA and Azide free

• WB

Lab

Western blot - Anti-NUDT5 antibody [EPR7735] - BSA and Azide free (AB248321)

This data was developed using the same antibody clone in a different buffer formulation (ab129163).

Lanes 1-4 : Merged signal (red and green). Green - ab129163 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.

ab129163 Anti-NUDT5 antibody [EPR7735] was shown to specifically react with NUDT5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266242 (knockout cell lysate ab258087) was used. Wild-type and NUDT5 knockout samples were subjected to SDS-PAGE. ab129163 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NUDT5 antibody [EPR7735] (<a href='/en-us/products/primary-antibodies/nudt5-antibody-epr7735-ab129163'>ab129163</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

NUDT5 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human NUDT5 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-nudt5-knockout-hek-293t-cell-line-ab266242'>ab266242</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 24 kDa

Observed band size: 30 kDa

false

• WB

Unknown

Western blot - Anti-NUDT5 antibody [EPR7735] - BSA and Azide free (AB248321)

This data was developed using ab129163, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NUDT5 antibody [EPR7735] (<a href='/en-us/products/primary-antibodies/nudt5-antibody-epr7735-ab129163'>ab129163</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

U937 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 24 kDa

Observed band size: 33 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] - BSA and Azide free (AB248321)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.