AB230313

Anti-Nuf2 antibody

Name: Anti-Nuf2 antibody (ab230313) | Abcam
Brand: Abcam Limited
SKU: AB230313
Price: 525 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal NUF2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUF2 aa 150 to C-terminus.

View Alternative Names

CDCA1, NUF2R, NUF2, Kinetochore protein Nuf2, hNuf2, hNuf2R, hsNuf2, Cell division cycle-associated protein 1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuf2 antibody (AB230313)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuf2 antibody (AB230313)

Paraffin-embedded human liver tissue stained for Nuf2 using ab230313 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Nuf2 antibody (AB230313)

All lanes:

Western blot - Anti-Nuf2 antibody (ab230313) at 1/1000 dilution

Lane 1:

Mouse liver lysate

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 3:

A431 (Human epidermoid carcinoma cell line) whole cell lysate

Lane 4:

PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

Lane 5:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 6:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 7:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 54 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuf2 antibody (AB230313)

Paraffin-embedded human skeletal muscle tissue stained for Nuf2 using ab230313 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NUF2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BZD4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nuf2 also known as CDCA1 is a protein involved in the regulation of mitosis. It weighs approximately 53 kDa and plays an important role in kinetochore function. You can find its expression in various human tissues with a high presence in dividing cells. This protein is part of the essential process of chromosome segregation during cell division.

Biological function summary

Nuf2 is necessary for maintaining mitotic spindle checkpoint fidelity. It forms part of the NDC80 complex an important player in chromosome alignment and separation. This complex contains other proteins such as HEC1 SPC25 and SPC24 and ensures that chromosomes are properly attached to spindle microtubules. Accurate spindle checkpoint functioning prevents errors that could lead to aneuploidy.

Pathways

Nuf2 participates in the mitotic spindle assembly checkpoint. It interacts with kinetochore proteins like HEC1 which integrates Nuf2's role in the chromosome passenger complex essential for proper mitotic progression. The NDC80 complex including Nuf2 is also linked to the cell cycle regulation pathway. This connection helps cells maintain genetic stability during division.

Nuf2's function and its role in cell division link it to cancer development. Its dysregulation can contribute to chromosomal instability a hallmark of many cancers. Nuf2's association with HEC1 highlights its aberrant expression in tumorigenesis. Additionally defects in its function can relate to neurodegenerative disorders like Alzheimer's disease as cellular division errors may cause neuronal loss or malfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed : 12438418, PubMed : 14654001, PubMed : 15062103, PubMed : 15235793, PubMed : 15239953, PubMed : 15548592, PubMed : 17535814). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed : 15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed : 23085020).

See full target information NUF2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:1057198 PubMed36620547

2022

NUF2 overexpression contributes to epithelial ovarian cancer progression ERBB3-mediated PI3K-AKT and MAPK signaling axes.

Applications

Unspecified application

Species

Unspecified reactive species

Ruobing Leng,Yunfang Meng,Xiaomei Sun,Yingzi Zhao

Cell death & disease 13:314 PubMed35393397

2022

A noncoding regulatory RNA Gm31932 induces cell cycle arrest and differentiation in melanoma via the miR-344d-3-5p/Prc1 (and Nuf2) axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Wang,Jianfei Chen,Bohan Li,Qingling Jiang,Ling Liu,Ziyi Xia,Qiusheng Zheng,Minjing Li,Defang Li

Frontiers in physiology 12:673497 PubMed34733169

2021

Distribution of Cardiac and Renal Corin and Proprotein Convertase Subtilisin/Kexin-6 in the Experimental Model of Cardio-Renal Syndrome of Various Severities.

Applications

Unspecified application

Species

Unspecified reactive species

Emad E Khoury,Ahmad Fokra,Safa Kinaneh,Yara Knaney,Doron Aronson,Zaid Abassi

Cancer cell international 20:251 PubMed32565735

2020

Random-forest algorithm based biomarkers in predicting prognosis in the patients with hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyun Guo,Zhenjiang Wang,Yuanyuan Du,Jie Mao,Junqiang Zhang,Zeyuan Yu,Jiwu Guo,Jun Zhao,Huinian Zhou,Haitao Wang,Yanmei Gu,Yumin Li

Nature communications 11:2652 PubMed32461611

2020

Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhei Yoshida,Sui Nishiyama,Lisa Lister,Shu Hashimoto,Tappei Mishina,Aurélien Courtois,Hirohisa Kyogoku,Takaya Abe,Aki Shiraishi,Meenakshi Choudhary,Yoshiharu Nakaoka,Mary Herbert,Tomoya S Kitajima

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nuf2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuf2 antibody (AB230313)

Western blot - Anti-Nuf2 antibody (AB230313)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuf2 antibody (AB230313)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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