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AB180945

Anti-Nuf2 antibody [EPR13019]

4

(1 Review)

|

(3 Publications)

Rabbit Recombinant Monoclonal NUF2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CDCA1, NUF2R, NUF2, Kinetochore protein Nuf2, hNuf2, hNuf2R, hsNuf2, Cell division cycle-associated protein 1

2 Images
Immunoprecipitation - Anti-Nuf2 antibody [EPR13019] (AB180945)
  • IP

Supplier Data

Immunoprecipitation - Anti-Nuf2 antibody [EPR13019] (AB180945)

Lysate from 293 cells was immunoprecipitated with ab180945 at a 1/50 dilution. For the subsequent blot, ab180945 used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer : 5% NFDM/TBST. Second lane is the negative control.

All lanes:

Immunoprecipitation - Anti-Nuf2 antibody [EPR13019] (ab180945)

Predicted band size: 54 kDa

false

Western blot - Anti-Nuf2 antibody [EPR13019] (AB180945)
  • WB

Supplier Data

Western blot - Anti-Nuf2 antibody [EPR13019] (AB180945)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Nuf2 antibody [EPR13019] (ab180945) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa

false

  • Carrier free

    Anti-Nuf2 antibody [EPR13019] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13019

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nuf2 also known as CDCA1 is a protein involved in the regulation of mitosis. It weighs approximately 53 kDa and plays an important role in kinetochore function. You can find its expression in various human tissues with a high presence in dividing cells. This protein is part of the essential process of chromosome segregation during cell division.
Biological function summary

Nuf2 is necessary for maintaining mitotic spindle checkpoint fidelity. It forms part of the NDC80 complex an important player in chromosome alignment and separation. This complex contains other proteins such as HEC1 SPC25 and SPC24 and ensures that chromosomes are properly attached to spindle microtubules. Accurate spindle checkpoint functioning prevents errors that could lead to aneuploidy.

Pathways

Nuf2 participates in the mitotic spindle assembly checkpoint. It interacts with kinetochore proteins like HEC1 which integrates Nuf2's role in the chromosome passenger complex essential for proper mitotic progression. The NDC80 complex including Nuf2 is also linked to the cell cycle regulation pathway. This connection helps cells maintain genetic stability during division.

Nuf2's function and its role in cell division link it to cancer development. Its dysregulation can contribute to chromosomal instability a hallmark of many cancers. Nuf2's association with HEC1 highlights its aberrant expression in tumorigenesis. Additionally defects in its function can relate to neurodegenerative disorders like Alzheimer's disease as cellular division errors may cause neuronal loss or malfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed : 12438418, PubMed : 14654001, PubMed : 15062103, PubMed : 15235793, PubMed : 15239953, PubMed : 15548592, PubMed : 17535814). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed : 15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed : 23085020).
See full target information NUF2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

NAR cancer 6:zcae015 PubMed38596432

2024

The DNA Damage Response (DDR) landscape of endometrial cancer defines discrete disease subtypes and reveals therapeutic opportunities.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyuan Zhang,Sayali Joseph,Di Wu,Jessica L Bowser,Cyrus Vaziri

International journal of oncology 57:1358-1367 PubMed33173994

2020

NUF2 as an anticancer therapeutic target and prognostic factor in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shanmei Lv,Wenjie Xu,Yanyan Zhang,Jiling Zhang,Xuejun Dong

The Journal of cell biology 209:221-34 PubMed25918224

2015

TRAMM/TrappC12 plays a role in chromosome congression, kinetochore stability, and CENP-E recruitment.

Applications

ICC/IF, WB

Species

Human, Human

Miroslav P Milev,Benedeta Hasaj,Djenann Saint-Dic,Sary Snounou,Qingchuan Zhao,Michael Sacher
View all publications
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Product promise

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