Rabbit Recombinant Monoclonal NUF2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Images
![Western blot - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (AB250277), expandable thumbnail](https://content.abcam.com/products/images/nuf2-antibody-epr13019-bsa-and-azide-free-ab250277--western-blot-img105113.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (AB250277)")
![Immunoprecipitation - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (AB250277), expandable thumbnail](https://content.abcam.com/products/images/nuf2-antibody-epr13019-bsa-and-azide-free-ab250277--immunoprecipitation-img65281.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (AB250277)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:12438418, PubMed:14654001, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:17535814). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).
Alternative names
CDCA1, NUF2R, NUF2, Kinetochore protein Nuf2, hNuf2, hNuf2R, hsNuf2, Cell division cycle-associated protein 1
Recommended products
Rabbit Recombinant Monoclonal NUF2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13019
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250277 is the carrier-free version of Anti-Nuf2 antibody [EPR13019] ab180945.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nuf2 also known as CDCA1 is a protein involved in the regulation of mitosis. It weighs approximately 53 kDa and plays an important role in kinetochore function. You can find its expression in various human tissues with a high presence in dividing cells. This protein is part of the essential process of chromosome segregation during cell division.
- Biological function summary
Nuf2 is necessary for maintaining mitotic spindle checkpoint fidelity. It forms part of the NDC80 complex an important player in chromosome alignment and separation. This complex contains other proteins such as HEC1 SPC25 and SPC24 and ensures that chromosomes are properly attached to spindle microtubules. Accurate spindle checkpoint functioning prevents errors that could lead to aneuploidy.
- Pathways
Nuf2 participates in the mitotic spindle assembly checkpoint. It interacts with kinetochore proteins like HEC1 which integrates Nuf2's role in the chromosome passenger complex essential for proper mitotic progression. The NDC80 complex including Nuf2 is also linked to the cell cycle regulation pathway. This connection helps cells maintain genetic stability during division.
- Associated diseases and disorders
Nuf2's function and its role in cell division link it to cancer development. Its dysregulation can contribute to chromosomal instability a hallmark of many cancers. Nuf2's association with HEC1 highlights its aberrant expression in tumorigenesis. Additionally defects in its function can relate to neurodegenerative disorders like Alzheimer's disease as cellular division errors may cause neuronal loss or malfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (ab250277), expandable thumbnail](https://content.abcam.com/products/images/nuf2-antibody-epr13019-bsa-and-azide-free-ab250277--western-blot-img105113.jpg "Western blot - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (ab250277)")
Western blot - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (ab250277)
This data was developed using Anti-Nuf2 antibody [EPR13019] ab180945, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Nuf2 antibody [EPR13019] (Anti-Nuf2 antibody [EPR13019] ab180945) at 1/1000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: A431 cell lysate at 20 µg
Lane 3: 293 cell lysate at 20 µg
Lane 4: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDa
![Immunoprecipitation - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (ab250277), expandable thumbnail](https://content.abcam.com/products/images/nuf2-antibody-epr13019-bsa-and-azide-free-ab250277--immunoprecipitation-img65281.jpg "Immunoprecipitation - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (ab250277)")
Immunoprecipitation - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (ab250277)
This data was developed using Anti-Nuf2 antibody [EPR13019] ab180945, the same antibody clone in a different buffer formulation.Lysate from 293 cells was immunoprecipitated with Anti-Nuf2 antibody [EPR13019] ab180945 at a 1/50 dilution. For the subsequent blot, Anti-Nuf2 antibody [EPR13019] ab180945 used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST. Second lane is the negative control.
All lanes: Immunoprecipitation - Anti-Nuf2 antibody [EPR13019] (Anti-Nuf2 antibody [EPR13019] ab180945)
Predicted band size: 54 kDa
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