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AB250277

Anti-Nuf2 antibody [EPR13019] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NUF2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CDCA1, NUF2R, NUF2, Kinetochore protein Nuf2, hNuf2, hNuf2R, hsNuf2, Cell division cycle-associated protein 1

2 Images
Immunoprecipitation - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (AB250277)
  • IP

Supplier Data

Immunoprecipitation - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (AB250277)

This data was developed using ab180945, the same antibody clone in a different buffer formulation.

Lysate from 293 cells was immunoprecipitated with ab180945 at a 1/50 dilution. For the subsequent blot, ab180945 used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer : 5% NFDM/TBST. Second lane is the negative control.

All lanes:

Immunoprecipitation - Anti-Nuf2 antibody [EPR13019] (<a href='/en-us/products/primary-antibodies/nuf2-antibody-epr13019-ab180945'>ab180945</a>)

Predicted band size: 54 kDa

false

Western blot - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (AB250277)
  • WB

Supplier Data

Western blot - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (AB250277)

This data was developed using ab180945, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Nuf2 antibody [EPR13019] (<a href='/en-us/products/primary-antibodies/nuf2-antibody-epr13019-ab180945'>ab180945</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13019

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab250277 is the carrier-free version of ab180945.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nuf2 also known as CDCA1 is a protein involved in the regulation of mitosis. It weighs approximately 53 kDa and plays an important role in kinetochore function. You can find its expression in various human tissues with a high presence in dividing cells. This protein is part of the essential process of chromosome segregation during cell division.
Biological function summary

Nuf2 is necessary for maintaining mitotic spindle checkpoint fidelity. It forms part of the NDC80 complex an important player in chromosome alignment and separation. This complex contains other proteins such as HEC1 SPC25 and SPC24 and ensures that chromosomes are properly attached to spindle microtubules. Accurate spindle checkpoint functioning prevents errors that could lead to aneuploidy.

Pathways

Nuf2 participates in the mitotic spindle assembly checkpoint. It interacts with kinetochore proteins like HEC1 which integrates Nuf2's role in the chromosome passenger complex essential for proper mitotic progression. The NDC80 complex including Nuf2 is also linked to the cell cycle regulation pathway. This connection helps cells maintain genetic stability during division.

Nuf2's function and its role in cell division link it to cancer development. Its dysregulation can contribute to chromosomal instability a hallmark of many cancers. Nuf2's association with HEC1 highlights its aberrant expression in tumorigenesis. Additionally defects in its function can relate to neurodegenerative disorders like Alzheimer's disease as cellular division errors may cause neuronal loss or malfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed : 12438418, PubMed : 14654001, PubMed : 15062103, PubMed : 15235793, PubMed : 15239953, PubMed : 15548592, PubMed : 17535814). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed : 15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed : 23085020).
See full target information NUF2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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