Rabbit Polyclonal NuMA antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NUMA1 aa 2050 to C-terminus.
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- Bioengineering & translational medicine 7:e103122022Engineering hypertrophic cartilage grafts from lipoaspirate for critical-sized calvarial bone defect reconstruction: An adipose tissue-based developmental engineering approach.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRu-Lin Huang,Rao Fu,Yuxin Yan,Chuanqi Liu,Jing Yang,Yun Xie,Qingfeng LiPubMed 36176620
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human NUMA1 aa 2050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14980
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
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7 products for Alternative Product
Target data
Function
Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division (PubMed:7769006, PubMed:17172455, PubMed:19255246, PubMed:24996901, PubMed:26195665, PubMed:27462074). Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles (PubMed:12445386, PubMed:11956313). Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner (PubMed:23870127, PubMed:24109598, PubMed:24996901, PubMed:26765568). In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle (PubMed:23027904, PubMed:22327364, PubMed:23921553). During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation (PubMed:22327364, PubMed:23921553, PubMed:24996901, PubMed:24371089). Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro (PubMed:24996901, PubMed:24371089). Also required for proper orientation of the mitotic spindle during asymmetric cell divisions (PubMed:21816348). Plays a role in mitotic MT aster assembly (PubMed:11163243, PubMed:11229403, PubMed:12445386). Involved in anastral spindle assembly (PubMed:25657325). Positively regulates TNKS protein localization to spindle poles in mitosis (PubMed:16076287). Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume (PubMed:10075938). Required for epidermal differentiation and hair follicle morphogenesis (By similarity).
Alternative names
Nuclear mitotic apparatus protein 1, Nuclear matrix protein-22, Nuclear mitotic apparatus protein, SP-H antigen, NMP-22, NuMA protein, NUMA, NMP22, NUMA1
Recommended products
Rabbit Polyclonal NuMA antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NUMA1 aa 2050 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human NUMA1 aa 2050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14980
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab241470 was affinity purified using an epitope specific to NuMA immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NuMA also known as "nuclear mitotic apparatus protein" is a large protein with a mass of approximately 250 kDa. It localizes in the nucleus and becomes highly concentrated at the spindle poles during mitosis. NuMA proteins appear in a variety of cells especially in cells undergoing division. Researchers often refer to it by alternate names such as "anti NuMA" when discussing its interactions in cellular contexts. Scientists have developed products such as NuMA polyclonal antibodies to study this protein's function.
- Biological function summary
The protein plays an important role in the organization of the mitotic spindle during cell division. It ensures that the spindle microtubules are correctly aligned and positioned. NuMA acts within a complex that includes dynein and dynactin facilitating proper chromosomal segregation. These actions are critical for maintaining genomic stability through accurate cell division.
- Pathways
NuMA's involvement in the cell cycle is significant especially within the mitotic checkpoint control pathways. It interacts closely with proteins like pericentrin and LGN ensuring the efficient completion of mitosis. These interactions confirm the protein's importance in pathway regulation particularly during transition phases within the cell cycle.
- Associated diseases and disorders
Abnormal NuMA function has associations with cancer and certain genetic disorders like microcephaly. Changes in NuMA expression or malfunction can disrupt normal spindle formation leading to chromosomal instability a common hallmark of cancer cells. Its interaction with proteins like p53 further connects it to oncogenic processes suggesting that understanding NuMA's pathways could contribute to therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody (ab241470)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for NuMA using ab241470 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
Western blot - Anti-NuMA antibody (ab241470)
All lanes: Western blot - Anti-NuMA antibody (ab241470) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 238 kDa
Exposure time: 30s
Immunoprecipitation - Anti-NuMA antibody (ab241470)
NuMA was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241470 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab241470 at 0.1 μg/ml.
Lane 1: ab241470 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-NuMA antibody (ab241470)
Predicted band size: 238 kDa
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