Rabbit Polyclonal NuMA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUMA1.
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- Nature communications 12:71572021NuMA regulates mitotic spindle assembly, structural dynamics and function via phase separation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengjie Sun,Mingkang Jia,He Ren,Biying Yang,Wangfei Chi,Guangwei Xin,Qing Jiang,Chuanmao ZhangPubMed 34887424
- Molecules (Basel, Switzerland) 25:2020AGS3 and Gα Are Concomitantly Upregulated as Part of the Spindle Orientation Complex during Differentiation of Human Neural Progenitor Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJackson L K Yip,Maggie M K Lee,Crystal C Y Leung,Man K Tse,Annie S T Cheung,Yung H WongPubMed 33172018
- The Journal of cell biology 219:2020The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Serra-Marques,Ronja Houtekamer,Dorine Hintzen,John T Canty,Ahmet Yildiz,Sophie DumontPubMed 33044554
- Cancer discovery 10:1352-13732020Limited Environmental Serine and Glycine Confer Brain Metastasis Sensitivity to PHGDH Inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBryan Ngo et. al.PubMed 32571778
- Biomaterials 240:1197912020Targeted camptothecin delivery via silicon nanoparticles reduces breast cancer metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarietta Landgraf,Christoph A Lahr,Ishdeep Kaur,Abbas Shafiee,Alvaro Sanchez-Herrero,Phillip W Janowicz,Akhilandeshwari Ravichandran,Christopher B Howard,Anna Cifuentes-Rius,Jacqui A McGovern,Nicolas H Voelcker,Dietmar W HutmacherPubMed 32109589
- Proceedings of the National Academy of Sciences of 117:1678-16882020Expansion, in vivo-ex vivo cycling, and genetic manipulation of primary human hepatocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEleftherios Michailidis et. al.PubMed 31915293
- Nature communications 11:712020Integrative discovery of treatments for high-risk neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElin Almstedt et. al.PubMed 31900415
- eLife 8:2019Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianlin Liang et. al.PubMed 31710289
- Endocrine-related cancer 27:23-392019Stem-like tumor cells involved in heterogeneous vasculogenesis in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuling Mao,Liuqing Zhu,Zhijian Huang,Chuanghua Luo,Ti Zhou,Lei Li,Guannan Wang,Zhonghan Yang,Weiwei Qi,Xia Yang,Guoquan GaoPubMed 31705798
- Cell 175:1591-1606.e192018Long-Term Expansion of Functional Mouse and Human Hepatocytes as 3D Organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuili Hu,Helmuth Gehart,Benedetta Artegiani,Carmen LÖpez-Iglesias,Florijn Dekkers,Onur Basak,Johan van Es,Susana M Chuva de Sousa Lopes,Harry Begthel,Jeroen Korving,Maaike van den Born,Chenhui Zou,Corrine Quirk,Luis Chiriboga,Charles M Rice,Stephanie Ma,Anne Rios,Peter J Peters,Ype P de Jong,Hans CleversPubMed 30500538
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NUMA1. The exact immunogen used to generate this antibody is proprietary information. Database link Q14980
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/200 | Notes - |
Associated Products
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Target data
Function
Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division (PubMed:17172455, PubMed:19255246, PubMed:24996901, PubMed:26195665, PubMed:27462074, PubMed:7769006). Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles (PubMed:11956313, PubMed:12445386). Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner (PubMed:23870127, PubMed:24109598, PubMed:24996901, PubMed:26765568). In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle (PubMed:22327364, PubMed:23027904, PubMed:23921553). During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation (PubMed:22327364, PubMed:23921553, PubMed:24371089, PubMed:24996901). Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro (PubMed:24371089, PubMed:24996901). Also required for proper orientation of the mitotic spindle during asymmetric cell divisions (PubMed:21816348). Plays a role in mitotic MT aster assembly (PubMed:11163243, PubMed:11229403, PubMed:12445386). Involved in anastral spindle assembly (PubMed:25657325). Positively regulates TNKS protein localization to spindle poles in mitosis (PubMed:16076287). Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume (PubMed:10075938). Required for epidermal differentiation and hair follicle morphogenesis (By similarity).
Alternative names
NMP22, NUMA, NUMA1, Nuclear mitotic apparatus protein 1, Nuclear matrix protein-22, Nuclear mitotic apparatus protein, SP-H antigen, NMP-22, NuMA protein
Recommended products
Rabbit Polyclonal NuMA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUMA1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NUMA1. The exact immunogen used to generate this antibody is proprietary information. Database link Q14980
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NuMA also known as "nuclear mitotic apparatus protein" is a large protein with a mass of approximately 250 kDa. It localizes in the nucleus and becomes highly concentrated at the spindle poles during mitosis. NuMA proteins appear in a variety of cells especially in cells undergoing division. Researchers often refer to it by alternate names such as "anti NuMA" when discussing its interactions in cellular contexts. Scientists have developed products such as NuMA polyclonal antibodies to study this protein's function.
- Biological function summary
The protein plays an important role in the organization of the mitotic spindle during cell division. It ensures that the spindle microtubules are correctly aligned and positioned. NuMA acts within a complex that includes dynein and dynactin facilitating proper chromosomal segregation. These actions are critical for maintaining genomic stability through accurate cell division.
- Pathways
NuMA's involvement in the cell cycle is significant especially within the mitotic checkpoint control pathways. It interacts closely with proteins like pericentrin and LGN ensuring the efficient completion of mitosis. These interactions confirm the protein's importance in pathway regulation particularly during transition phases within the cell cycle.
- Associated diseases and disorders
Abnormal NuMA function has associations with cancer and certain genetic disorders like microcephaly. Changes in NuMA expression or malfunction can disrupt normal spindle formation leading to chromosomal instability a common hallmark of cancer cells. Its interaction with proteins like p53 further connects it to oncogenic processes suggesting that understanding NuMA's pathways could contribute to therapeutic strategies.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-NuMA antibody (ab97585)
ab97585, at 1/200, staining paraformaldehyde-fixed A431 cells by Immunofluorescence (Panel 1) and merged with DNA probe (Panel 2).
Western blot - Anti-NuMA antibody (ab97585)
7.5% SDS PAGE
All lanes: Western blot - Anti-NuMA antibody (ab97585) at 1/1000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 238 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody (ab97585)
ab97585, at 1/100, staining paraffin-embedded Cal27 Xenograft by Immunohistochemistry.
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