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AB155415

Anti-NUMB antibody

5

(1 Review)

|

(3 Publications)

Rabbit Polyclonal NUMB antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUMB aa 1-250.

View Alternative Names

C14orf41, NUMB, Protein numb homolog, h-Numb, Protein S171

3 Images
Western blot - Anti-NUMB antibody (AB155415)
  • WB

Unknown

Western blot - Anti-NUMB antibody (AB155415)

7.5% SDS PAGE

All lanes:

Western blot - Anti-NUMB antibody (ab155415) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 71 kDa

false

Western blot - Anti-NUMB antibody (AB155415)
  • WB

Supplier Data

Western blot - Anti-NUMB antibody (AB155415)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-NUMB antibody (ab155415) at 1/1000 dilution

All lanes:

Mouse lung tissue lysate at 50 µg

Predicted band size: 71 kDa

false

Western blot - Anti-NUMB antibody (AB155415)
  • WB

Supplier Data

Western blot - Anti-NUMB antibody (AB155415)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-NUMB antibody (ab155415) at 1/1000 dilution

All lanes:

Rat lung tissue lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human NUMB aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P49757

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUMB also known as numb protein is a cytoplasmic protein involved in cell fate determination. It has an approximate molecular mass of 75 kDa. NUMB plays a critical role in asymmetric cell division particularly during neural development. The protein is expressed in various tissues including the brain heart and liver but most prominently in neural tissues. NUMB influences the timing of differentiation and proliferation by interacting with several cellular components.
Biological function summary

NUMB proteins are important to regulating cell fate decisions. NUMB primarily functions as a part of a larger complex including proteins like Notch and Eps15. In neural stem cells NUMB acts as an inhibitor of the Notch signaling pathway. It also regulates endocytosis and the signaling pathways that contribute to maintaining cellular diversity during development. NUMB interactions influence processes like cell adhesion and migration which are essential for proper tissue formation.

Pathways

NUMB proteins play significant roles within the Notch and Hedgehog signaling pathways. These are critical pathways for developmental processes. In the Notch pathway NUMB negatively regulates Notch receptor activity preventing Notch-mediated gene expression. NUMB interacts with other proteins such as alpha-adaptin and LNX1 through these pathways helping regulate the endocytic trafficking of key signaling molecules. In the Hedgehog pathway NUMB affects signal transduction though its role is less defined compared to its involvement in the Notch pathway.

NUMB proteins have been implicated in breast cancer and Alzheimer's disease. In breast cancer alterations in NUMB expression or function can contribute to tumor progression due to dysregulation of the Notch pathway. Its interaction with proteins like MDM2 influences the stability and activity of p53 an important tumor suppressor. In Alzheimer's disease NUMB's involvement in endocytic processes may impact amyloid precursor protein processing and beta-amyloid plaque formation which are central to the disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates clathrin-mediated receptor endocytosis (PubMed : 18657069). Plays a role in the process of neurogenesis (By similarity). Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate (By similarity). Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity (By similarity). May also mediate local repair of brain ventricular wall damage (By similarity).
See full target information NUMB
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:12572-12582 PubMed35599603

2022

SLC8A1 antisense RNA 1 suppresses papillary thyroid cancer malignant progression via the FUS RNA binding protein (FUS)/NUMB like endocytic adaptor protein (Numbl) axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yunchao Xin,Xiaoling Shang,Xiaoran Sun,Guogang Xu,Yachao Liu,Yanbin Liu

eLife 9: PubMed32142410

2020

Presynaptic PTPσ regulates postsynaptic NMDA receptor function through direct adhesion-independent mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Kyungdeok Kim,Wangyong Shin,Muwon Kang,Suho Lee,Doyoun Kim,Ryeonghwa Kang,Yewon Jung,Yisul Cho,Esther Yang,Hyun Kim,Yong Chul Bae,Eunjoon Kim

Oncology letters 18:3646-3654 PubMed31579077

2019

All-trans retinoic acid therapy induces asymmetric division of glioma stem cells from the U87MG cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Ruizhi Wang,Chongxiao Liu
View all publications
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Product promise

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