Goat Polyclonal NUMB antibody. Suitable for Flow Cyt, IP, ELISA, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Chicken samples. Cited in 41 publications. Immunogen corresponding to Synthetic Peptide within Human NUMB aa 600 to C-terminus.
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Publications
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- Nature communications 14:22902023RNA binding protein SYNCRIP maintains proteostasis and self-renewal of hematopoietic stem and progenitor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlorisela Herrejon Chavez,Hanzhi Luo,Paolo Cifani,Alli Pine,Karen L Chu,Suhasini Joshi,Ersilia Barin,Alexandra Schurer,Mandy Chan,Kathryn Chang,Grace Y Q Han,Aspen J Pierson,Michael Xiao,Xuejing Yang,Lindsey M Kuehm,Yuning Hong,Diu T T Nguyen,Gabriela Chiosis,Alex Kentsis,Christina Leslie,Ly P Vu,Michael G KharasPubMed 37085479
- International journal of biological sciences 18:1896-19112022MAD2B promotes podocyte injury through regulating Numb-dependent Notch 1 pathway in diabetic nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng-Ran Li,Chun-Tao Lei,Hui Tang,Xing-Jie Yin,Zhe Hao,Yang Qiu,Ya-Ru Xie,Jie-Yu Zeng,Hua Su,Chun ZhangPubMed 35342338
- eLife 10:2021DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEva On-Chai Lau,Devid Damiani,Georges Chehade,Nuria Ruiz-Reig,Rana Saade,Yves Jossin,Mohamed Aittaleb,Olivier Schakman,Nicolas Tajeddine,Philippe Gailly,Fadel TissirPubMed 33899739
- Nature communications 12:10652021LIGHT/LTβR signaling regulates self-renewal and differentiation of hematopoietic and leukemia stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS S Höpner,Ana Raykova,R Radpour,M A Amrein,D Koller,G M Baerlocher,C Riether,A F OchsenbeinPubMed 33594067
- Aging cell 20:e133102021Decreased p53 is associated with a decline in asymmetric stem cell self-renewal in aged human epidermis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra Charruyer,Tracy Weisenberger,Hang Li,Ayman Khalifa,Andrew W Schroeder,Annika Belzer,Ruby GhadiallyPubMed 33524216
- Molecular brain 14:202021Non-SUMOylated CRMP2 decreases Na1.7 currents via the endocytic proteins Numb, Nedd4-2 and Eps15.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKimberly Gomez et. al.PubMed 33478555
- Cell reports 30:3605-3615.e52020A Model of Differential Mammary Growth Initiation by Stat3 and Asymmetric Integrin-α6 Inheritance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdward J Morris,Jordan A Gillespie,Christopher A Maxwell,Shoukat DedharPubMed 32187533
- American journal of physiology. Gastrointestinal a 318:G841-G8532020negatively regulates the epithelial-to-mesenchymal transition in colorectal cancer through the Wnt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChi Cheng et. al.PubMed 32146835
- GeroScience 42:595-6112020CD82-TRPM7-Numb signaling mediates age-related cognitive impairment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYin Zhao et. al.PubMed 32088828
- Oncogenesis 9:202020CUL4B contributes to cancer stemness by repressing tumor suppressor miR34a in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanjun Li et. al.PubMed 32054830
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NUMB aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P49757
Reactivity data
Select an application| Flow Cyt | IP | ELISA | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested | Expected | Tested |
| Chicken | Predicted | Expected | Expected | Predicted | Expected | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Chicken | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes For 1 hr on mouse brain lysate (see Abreview). |
Species Chicken | Dilution info - | Notes For 1 hr on mouse brain lysate (see Abreview). |
Species Human | Dilution info - | Notes For 1 hr on mouse brain lysate (see Abreview). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-2.00000 µg/mL | Notes 1 hour primary incubation at room temperature is recommended for this product. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Chicken | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 8 µg/mL | Notes See Abreview. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes See Abreview. |
Species Chicken | Dilution info - | Notes See Abreview. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 10 µg/mL | Notes - |
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Chicken | Dilution info - | Notes - |
Associated Products
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Target data
Function
Regulates clathrin-mediated receptor endocytosis (PubMed:18657069). Plays a role in the process of neurogenesis (By similarity). Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate (By similarity). Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity (By similarity). May also mediate local repair of brain ventricular wall damage (By similarity).
Alternative names
C14orf41, NUMB, Protein numb homolog, h-Numb, Protein S171
Recommended products
Goat Polyclonal NUMB antibody. Suitable for Flow Cyt, IP, ELISA, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Chicken samples. Cited in 41 publications. Immunogen corresponding to Synthetic Peptide within Human NUMB aa 600 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NUMB aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P49757
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product may cross-react with Numblike (NUMBL, GeneID: 9253)
Principal Names - NUMB; S171; numb homolog (Drosophila)
Official Gene Symbol - NUMB
GenBank Accession Number – NP_003735
LocusLink ID - 8650 (human); 18222 (mouse)
Gene Ontology terms - integral to plasma membrane
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NUMB also known as numb protein is a cytoplasmic protein involved in cell fate determination. It has an approximate molecular mass of 75 kDa. NUMB plays a critical role in asymmetric cell division particularly during neural development. The protein is expressed in various tissues including the brain heart and liver but most prominently in neural tissues. NUMB influences the timing of differentiation and proliferation by interacting with several cellular components.
- Biological function summary
NUMB proteins are important to regulating cell fate decisions. NUMB primarily functions as a part of a larger complex including proteins like Notch and Eps15. In neural stem cells NUMB acts as an inhibitor of the Notch signaling pathway. It also regulates endocytosis and the signaling pathways that contribute to maintaining cellular diversity during development. NUMB interactions influence processes like cell adhesion and migration which are essential for proper tissue formation.
- Pathways
NUMB proteins play significant roles within the Notch and Hedgehog signaling pathways. These are critical pathways for developmental processes. In the Notch pathway NUMB negatively regulates Notch receptor activity preventing Notch-mediated gene expression. NUMB interacts with other proteins such as alpha-adaptin and LNX1 through these pathways helping regulate the endocytic trafficking of key signaling molecules. In the Hedgehog pathway NUMB affects signal transduction though its role is less defined compared to its involvement in the Notch pathway.
- Associated diseases and disorders
NUMB proteins have been implicated in breast cancer and Alzheimer's disease. In breast cancer alterations in NUMB expression or function can contribute to tumor progression due to dysregulation of the Notch pathway. Its interaction with proteins like MDM2 influences the stability and activity of p53 an important tumor suppressor. In Alzheimer's disease NUMB's involvement in endocytic processes may impact amyloid precursor protein processing and beta-amyloid plaque formation which are central to the disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Western blot - Anti-NUMB antibody (ab4147)
Detected by chemiluminescence.
All lanes: Western blot - Anti-NUMB antibody (ab4147) at 1 µg/mL
All lanes: Mouse brain lysates at 35 µg
Predicted band size: 71 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cell junction and cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)
Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)
ab4147 staining NUMB in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUMB antibody (ab4147)
Immunohistochemistry staining of paraffin embedded Human Prostate labeling NUMB with ab4147 at (8µg/mL). Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry - Anti-NUMB antibody (ab4147)
Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Sandwich ELISA - Anti-NUMB antibody (ab4147)
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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