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AB114096

Anti-NUP133 antibody

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(1 Publication)

Rabbit Polyclonal NUP133 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Nuclear pore complex protein Nup133 aa 650-700.

View Alternative Names

Nuclear pore complex protein Nup133, 133 kDa nucleoporin, Nucleoporin Nup133, NUP133

1 Images
Immunoprecipitation - Anti-NUP133 antibody (AB114096)
  • IP

Unknown

Immunoprecipitation - Anti-NUP133 antibody (AB114096)

A rabbit anti-NUP133 antibody, at 1 µg/ml, staining NUP133 in HeLa whole cell lysate immunoprecipitated using ab114096 at 10 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-NUP133 antibody (ab114096)

Predicted band size: 129 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human Nuclear pore complex protein Nup133 aa 650-700. The exact immunogen used to generate this antibody is proprietary information.

Q8WUM0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab114096 was affinity purified using an epitope specific to NUP133 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUP133 also known as nucleoporin 133 is a component of the nuclear pore complex. This protein weighs approximately 133 kDa. NUP133 localizes to the nuclear envelope and forms part of the structural scaffold of the nuclear pore. You will find expressions of this protein in a variety of cell types across different tissues. As an integral component of the nuclear pore complex NUP133 plays a role in transporting molecules between the nucleus and the cytoplasm.
Biological function summary

This protein is part of the Nup107-160 complex. This complex is essential for the assembly and maintenance of nuclear pores. NUP133 contributes to regulating the bidirectional transport of proteins and RNA across the nuclear envelope. It is involved in maintaining the structural integrity of the nuclear pore complex ensuring the selective exchange of substances between the nucleus and the cytoplasm. By doing so NUP133 influences various cellular processes like gene expression and DNA replication.

Pathways

The protein NUP133 engages primarily in the nucleocytoplasmic transport pathway. Within this framework it collaborates closely with other nucleoporins such as NUP107 and NUP160. The protein is also relevant in the regulation of the cell cycle as it ensures the proper formation and disassembly of nuclear pores during cell division. The interaction with other proteins within these pathways highlights the critical roles NUP133 fulfills in fundamental cellular activities.

Studies link NUP133 to certain cancer types including ovarian cancer and glioblastoma. Mutations or misregulation of NUP133 can lead to disturbances in nuclear transport and cellular proliferation contributing to tumorigenesis. The protein interacts with other members of the nuclear pore complex such as NUP85 underlining its potential impact in cancer progression. Understanding these associations may inform therapeutic strategies targeting nuclear transport pathways in related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in poly(A)+ RNA transport. Involved in nephrogenesis (PubMed : 30179222).
See full target information Nuclear pore complex protein Nup133
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 8:130 PubMed36473865

2022

The chromatin remodeler CHD6 promotes colorectal cancer development by regulating TMEM65-mediated mitochondrial dynamics via EGF and Wnt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Boyu Zhang,Qingxin Liu,Weijie Wen,Han Gao,Wenxia Wei,Aiwen Tang,Baifu Qin,Haiwen Lyu,Xiangqi Meng,Kai Li,Huilin Jin,Fenghai Yu,Qihao Pan,Junzhong Lin,Mong-Hong Lee
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Product promise

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