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AB155990

Anti-NUP133 antibody [EPR10808(B)]

5

(2 Reviews)

|

(15 Publications)

Rabbit Recombinant Monoclonal NUP133 antibody. Suitable for WB, ICC/IF and reacts with African green monkey, Human samples. Cited in 15 publications.

View Alternative Names

Nuclear pore complex protein Nup133, 133 kDa nucleoporin, Nucleoporin Nup133, NUP133

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (AB155990)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (AB155990)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling NUP133 with purified ab155990 at 1 : 50 (6.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (AB155990)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (AB155990)

Immunofluorescent analysis of HeLa cells labeling NUP133 with unpurified ab155990 at 1/50 dilution. This image was produced using unpurified antibody.

Western blot - Anti-NUP133 antibody [EPR10808(B)] (AB155990)
  • WB

Unknown

Western blot - Anti-NUP133 antibody [EPR10808(B)] (AB155990)

This image was produced using unpurified antibody.

All lanes:

Western blot - Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 129 kDa

false

Western blot - Anti-NUP133 antibody [EPR10808(B)] (AB155990)
  • WB

Unknown

Western blot - Anti-NUP133 antibody [EPR10808(B)] (AB155990)

All lanes:

Western blot - Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/3000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 20 µg

Lane 3:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 129 kDa

Observed band size: 129 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (AB155990)
  • ICC/IF

AbReview45106****

Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (AB155990)

Unpurified ab155990 staining NUP133 in monkey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 μg/ml) was used as the secondary antibody. This image was produced using unpurified antibody.

This image is courtesy of an Abreview submitted by Aaron Halpen

Western blot - Anti-NUP133 antibody [EPR10808(B)] (AB155990)
  • WB

Unknown

Western blot - Anti-NUP133 antibody [EPR10808(B)] (AB155990)

All lanes:

Western blot - Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilution

All lanes:

COS-1 (African green monkey kidney fibroblast-like) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 129 kDa

Observed band size: 129 kDa

false

  • Carrier free

    Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-NUP133 antibody [EPR10808(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10808(B)

Isotype

IgG

Carrier free

No

Reacts with

Human, African green monkey

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "African green monkey": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUP133 also known as nucleoporin 133 is a component of the nuclear pore complex. This protein weighs approximately 133 kDa. NUP133 localizes to the nuclear envelope and forms part of the structural scaffold of the nuclear pore. You will find expressions of this protein in a variety of cell types across different tissues. As an integral component of the nuclear pore complex NUP133 plays a role in transporting molecules between the nucleus and the cytoplasm.
Biological function summary

This protein is part of the Nup107-160 complex. This complex is essential for the assembly and maintenance of nuclear pores. NUP133 contributes to regulating the bidirectional transport of proteins and RNA across the nuclear envelope. It is involved in maintaining the structural integrity of the nuclear pore complex ensuring the selective exchange of substances between the nucleus and the cytoplasm. By doing so NUP133 influences various cellular processes like gene expression and DNA replication.

Pathways

The protein NUP133 engages primarily in the nucleocytoplasmic transport pathway. Within this framework it collaborates closely with other nucleoporins such as NUP107 and NUP160. The protein is also relevant in the regulation of the cell cycle as it ensures the proper formation and disassembly of nuclear pores during cell division. The interaction with other proteins within these pathways highlights the critical roles NUP133 fulfills in fundamental cellular activities.

Studies link NUP133 to certain cancer types including ovarian cancer and glioblastoma. Mutations or misregulation of NUP133 can lead to disturbances in nuclear transport and cellular proliferation contributing to tumorigenesis. The protein interacts with other members of the nuclear pore complex such as NUP85 underlining its potential impact in cancer progression. Understanding these associations may inform therapeutic strategies targeting nuclear transport pathways in related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in poly(A)+ RNA transport. Involved in nephrogenesis (PubMed : 30179222).
See full target information Nuclear pore complex protein Nup133
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Marine drugs 23: PubMed40137294

2025

The Polybrominated Diphenyl Ether Bromoxib Disrupts Nuclear Import and Export by Affecting Nucleoporins of the Nuclear Pore Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Karina S Krings,Anastasia Ritchie,Laura Schmitt,Judith Hatzfeld,Gudrun Totzke,Thomas Lenz,María José Mendiburo,Björn Stork,Nicole Teusch,Peter Proksch,Kai Stühler,Lisa Müller,Sebastian Wesselborg

Nature cell biology 26:1943-1957 PubMed39482354

2024

Caspase-2 is a condensate-mediated deubiquitinase in protein quality control.

Applications

Unspecified application

Species

Unspecified reactive species

Yingwei Ge,Lijie Zhou,Yesheng Fu,Lijuan He,Yi Chen,Dingchang Li,Yuping Xie,Jun Yang,Haitao Wu,Hongmiao Dai,Zhiqiang Peng,Yong Zhang,Shaoqiong Yi,Bo Wu,Xin Zhang,Yangjun Zhang,Wantao Ying,Chun-Ping Cui,Cui Hua Liu,Lingqiang Zhang

The Journal of cell biology 223: PubMed38652117

2024

UBAP2L ensures homeostasis of nuclear pore complexes at the intact nuclear envelope.

Applications

Unspecified application

Species

Unspecified reactive species

Yongrong Liao,Leonid Andronov,Xiaotian Liu,Junyan Lin,Lucile Guerber,Linjie Lu,Arantxa Agote-Arán,Evanthia Pangou,Li Ran,Charlotte Kleiss,Mengdi Qu,Stephane Schmucker,Luca Cirillo,Zhirong Zhang,Daniel Riveline,Monica Gotta,Bruno P Klaholz,Izabela Sumara

Nucleic acids research 51:10568-10589 PubMed37739411

2023

The TRIM69-MST2 signaling axis regulates centrosome dynamics and chromosome segregation.

Applications

Unspecified application

Species

Unspecified reactive species

Yilin Wang,Patrik Risteski,Yang Yang,Huan Chen,Gaith Droby,Andrea Walens,Deepika Jayaprakash,Melissa Troester,Laura Herring,Jonathan Chernoff,Iva M Tolić,Jessica Bowser,Cyrus Vaziri

Developmental cell 58:847-865.e10 PubMed37098350

2023

A tubule-sheet continuum model for the mechanism of nuclear envelope assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Gengjing Zhao,Shiwei Liu,Sanjana Arun,Fioranna Renda,Alexey Khodjakov,David Pellman

Current biology : CB 33:912-925.e6 PubMed36720222

2023

Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression.

Applications

Unspecified application

Species

Unspecified reactive species

Jingchao Wu,Ainhoa Larreategui-Aparicio,Maaike L A Lambers,Dani L Bodor,Sjoerd J Klaasen,Eveline Tollenaar,Marta de Ruijter-Villani,Geert J P L Kops

eLife 11: PubMed35834310

2022

Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Shi,Hieng Chiong Tie,Mahajan Divyanshu,Xiuping Sun,Yan Zhou,Boon Kim Boh,Leah A Vardy,Lei Lu

Nature communications 13:204 PubMed35017527

2022

Canonical WNT signaling-dependent gating of MYC requires a noncanonical CTCF function at a distal binding site.

Applications

Unspecified application

Species

Unspecified reactive species

Ilyas Chachoua,Ilias Tzelepis,Hao Dai,Jia Pei Lim,Anna Lewandowska-Ronnegren,Felipe Beccaria Casagrande,Shuangyang Wu,Johanna Vestlund,Carolina Diettrich Mallet de Lima,Deeksha Bhartiya,Barbara A Scholz,Mirco Martino,Rashid Mehmood,Anita Göndör

Life science alliance 5: PubMed34789512

2021

Chromosome length and gene density contribute to micronuclear membrane stability.

Applications

Unspecified application

Species

Unspecified reactive species

Anna E Mammel,Heather Z Huang,Amanda L Gunn,Emma Choo,Emily M Hatch

Developmental cell 56:1786-1803.e9 PubMed34129835

2021

Inherited nuclear pore substructures template post-mitotic pore assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ying Chou,Srigokul Upadhyayula,Justin Houser,Kangmin He,Wesley Skillern,Gustavo Scanavachi,Song Dang,Anwesha Sanyal,Kazuka G Ohashi,Giuseppe Di Caprio,Alex J B Kreutzberger,Tegy John Vadakkan,Tom Kirchhausen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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