AB181355

Anti-NUP133 antibody [EPR10809]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal NUP133 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

Nuclear pore complex protein Nup133, 133 kDa nucleoporin, Nucleoporin Nup133, NUP133

1 Images

Supplier Data

Western blot - Anti-NUP133 antibody [EPR10809] (AB181355)

All lanes:

Western blot - Anti-NUP133 antibody [EPR10809] (ab181355) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Predicted band size: 129 kDa

false

Carrier free

Anti-NUP133 antibody [EPR10809] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10809

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUP133 also known as nucleoporin 133 is a component of the nuclear pore complex. This protein weighs approximately 133 kDa. NUP133 localizes to the nuclear envelope and forms part of the structural scaffold of the nuclear pore. You will find expressions of this protein in a variety of cell types across different tissues. As an integral component of the nuclear pore complex NUP133 plays a role in transporting molecules between the nucleus and the cytoplasm.

Biological function summary

This protein is part of the Nup107-160 complex. This complex is essential for the assembly and maintenance of nuclear pores. NUP133 contributes to regulating the bidirectional transport of proteins and RNA across the nuclear envelope. It is involved in maintaining the structural integrity of the nuclear pore complex ensuring the selective exchange of substances between the nucleus and the cytoplasm. By doing so NUP133 influences various cellular processes like gene expression and DNA replication.

Pathways

The protein NUP133 engages primarily in the nucleocytoplasmic transport pathway. Within this framework it collaborates closely with other nucleoporins such as NUP107 and NUP160. The protein is also relevant in the regulation of the cell cycle as it ensures the proper formation and disassembly of nuclear pores during cell division. The interaction with other proteins within these pathways highlights the critical roles NUP133 fulfills in fundamental cellular activities.

Studies link NUP133 to certain cancer types including ovarian cancer and glioblastoma. Mutations or misregulation of NUP133 can lead to disturbances in nuclear transport and cellular proliferation contributing to tumorigenesis. The protein interacts with other members of the nuclear pore complex such as NUP85 underlining its potential impact in cancer progression. Understanding these associations may inform therapeutic strategies targeting nuclear transport pathways in related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in poly(A)+ RNA transport. Involved in nephrogenesis (PubMed : 30179222).

See full target information Nuclear pore complex protein Nup133

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 136: PubMed37305998

2023

Y-complex nucleoporins independently contribute to nuclear pore assembly and gene regulation in neuronal progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Clarisse Orniacki,Annalisa Verrico,Stéphane Pelletier,Benoit Souquet,Fanny Coulpier,Laurent Jourdren,Serena Benetti,Valérie Doye

Scientific reports 9:4750 PubMed30894603

2019

Moderate Nucleoporin 133 deficiency leads to glomerular damage in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Cianciolo Cosentino,Alessandro Berto,Stéphane Pelletier,Michelle Hari,Johannes Loffing,Stephan C F Neuhauss,Valérie Doye

Annals of neurology 84:814-828 PubMed30427554

2018

Homozygous splicing mutation in NUP133 causes Galloway-Mowat syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Atsushi Fujita,Hiroyasu Tsukaguchi,Eriko Koshimizu,Hitoshi Nakazato,Kyoko Itoh,Shohei Kuraoka,Yoshihiro Komohara,Masaaki Shiina,Shohei Nakamura,Mika Kitajima,Yoshinori Tsurusaki,Satoko Miyatake,Kazuhiro Ogata,Kazumoto Iijima,Naomichi Matsumoto,Noriko Miyake

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUP133 antibody [EPR10809]

Western blot - Anti-NUP133 antibody [EPR10809] (AB181355)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Product promise