AB245668

Anti-Nup153 antibody

Name: Anti-Nup153 antibody (ab245668) | Abcam
Brand: Abcam Limited
SKU: AB245668
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Nup153 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NUP153 aa 50-100.

View Alternative Names

Nuclear pore complex protein Nup153, 153 kDa nucleoporin, Nucleoporin Nup153, NUP153

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody (AB245668)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody (AB245668)

Formaldehyde-fixed asynchronous HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Nup153 using ab245668 at a 1/100 dilution in ICC/IF.

Detection : Red-fluorescent goat anti-rabbit IgG, highly cross-absorbed.

Supplier Data

Immunoprecipitation - Anti-Nup153 antibody (AB245668)

Nup153 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

ab245668 used for IP at 3 μg/mg lysate. For WB 1 μg/ml.

Lane 1 : ab245668 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Chemiluminescence detection : 1 second.

All lanes:

Immunoprecipitation - Anti-Nup153 antibody (ab245668)

Predicted band size: 154 kDa

false

Supplier Data

Western blot - Anti-Nup153 antibody (AB245668)

All lanes:

Western blot - Anti-Nup153 antibody (ab245668) at 0.04 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 154 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IP

applications

Immunogen

Synthetic Peptide within Human NUP153 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P49790

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab245668 was affinity purified using an epitope specific to Nup153 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nup153 also known as nucleoporin 153 is a component of the nuclear pore complex (NPC) with a molecular weight of approximately 153 kDa. It functions as a scaffold within the NPC which mediates the bidirectional transport of molecules between the nucleus and the cytoplasm. Nup153 is part of the FG-nucleoporin family characterized by domains rich in phenylalanine and glycine repeats. It is highly expressed in the nuclear envelope of eukaryotic cells playing a vital part in maintaining the permeability of the nuclear envelope.

Biological function summary

Nup153 contributes to the assembly and disassembly of the NPC during cell cycle progression. It acts as a docking site for various nuclear import and export receptors therefore is essential for nucleocytoplasmic transport. Nup153 is an important component in the larger nuclear pore complex which itself is made up of multiple nucleoporins forming a sophisticated structure capable of both passive and active transport processes.

Pathways

Nup153 is intricately involved in the nucleocytoplasmic transport pathway and plays a critical role in RNA processing. It interacts with proteins such as Importin β and Exportin which facilitate the transport of different molecules across the nuclear envelope. This pathway is fundamental for the proper regulation of gene expression and cell cycle control exhibiting the importance of Nup153 in cellular dynamics.

Nup153 has been linked to cancer and neurodegenerative diseases. Alterations in Nup153 expression or function can disrupt cellular homeostasis leading to uncontrolled cell proliferation or impaired neuronal function. In cancer it may show altered interactions with p53 an important tumor suppressor protein affecting its regulatory functions. Understanding the role of Nup153 in such diseases may offer insights into potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Mediates TPR anchoring to the nuclear membrane at NPC. The repeat-containing domain may be involved in anchoring other components of the NPC to the pore membrane. Possible DNA-binding subunit of the nuclear pore complex (NPC).. (Microbial infection) Interacts with HIV-1 capsid protein P24 and thereby promotes the integration of the virus in the nucleus of non-dividing cells (in vitro).. (Microbial infection) Binds HIV-2 protein vpx and thereby promotes the nuclear translocation of the lentiviral genome (in vitro).

See full target information NUP153

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 22:24 PubMed40260072

2025

Interactions of tumor necrosis factor receptor-associated factor 4 and pyruvate kinase muscle isoform 2 promote malignant behavior and aerobic glycolysis in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tongming Liu,Shihong Zhu,Jiawei Sun,Yuanyuan Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nup153 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody (AB245668)

Immunoprecipitation - Anti-Nup153 antibody (AB245668)

Western blot - Anti-Nup153 antibody (AB245668)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise