JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB84872

Anti-Nup153 antibody

5

(7 Reviews)

|

(22 Publications)

Rabbit Polyclonal Nup153 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 22 publications. Immunogen corresponding to Synthetic Peptide within Human NUP153 aa 50-100.

View Alternative Names

Nuclear pore complex protein Nup153, 153 kDa nucleoporin, Nucleoporin Nup153, NUP153

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody (AB84872)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody (AB84872)

Staining of human NUP153 in NBF fixed, asynchronous HeLa cells by Immunocytochemistry, using ab84872 at a dilution of 1/250. Detection : Red fluorescent goat anti-rabbit IgG used at a dilution of 1/100.

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody (AB84872)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody (AB84872)

Staining of human NUP153 in NBF fixed asynchronous HeLa cells by Immunocytochemistry, using ab84872 at a dilution of 1/250. Detection : Red-fluorescent goat anti-rabbit IgG used at a dilution of 1/100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human NUP153 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P49790

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p>Formaldehyde fixed cells. Permeabilization with TritonX-100 is recommended. Acetone fixation is not recommended.</p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Concentration is optimized for IHC and not determined
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified using an epitope specific to NUP153 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nup153 also known as nucleoporin 153 is a component of the nuclear pore complex (NPC) with a molecular weight of approximately 153 kDa. It functions as a scaffold within the NPC which mediates the bidirectional transport of molecules between the nucleus and the cytoplasm. Nup153 is part of the FG-nucleoporin family characterized by domains rich in phenylalanine and glycine repeats. It is highly expressed in the nuclear envelope of eukaryotic cells playing a vital part in maintaining the permeability of the nuclear envelope.
Biological function summary

Nup153 contributes to the assembly and disassembly of the NPC during cell cycle progression. It acts as a docking site for various nuclear import and export receptors therefore is essential for nucleocytoplasmic transport. Nup153 is an important component in the larger nuclear pore complex which itself is made up of multiple nucleoporins forming a sophisticated structure capable of both passive and active transport processes.

Pathways

Nup153 is intricately involved in the nucleocytoplasmic transport pathway and plays a critical role in RNA processing. It interacts with proteins such as Importin β and Exportin which facilitate the transport of different molecules across the nuclear envelope. This pathway is fundamental for the proper regulation of gene expression and cell cycle control exhibiting the importance of Nup153 in cellular dynamics.

Nup153 has been linked to cancer and neurodegenerative diseases. Alterations in Nup153 expression or function can disrupt cellular homeostasis leading to uncontrolled cell proliferation or impaired neuronal function. In cancer it may show altered interactions with p53 an important tumor suppressor protein affecting its regulatory functions. Understanding the role of Nup153 in such diseases may offer insights into potential therapeutic interventions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Mediates TPR anchoring to the nuclear membrane at NPC. The repeat-containing domain may be involved in anchoring other components of the NPC to the pore membrane. Possible DNA-binding subunit of the nuclear pore complex (NPC).. (Microbial infection) Interacts with HIV-1 caspid protein P24 and thereby promotes the integration of the virus in the nucleus of non-dividing cells (in vitro).. (Microbial infection) Binds HIV-2 protein vpx and thereby promotes the nuclear translocation of the lentiviral genome (in vitro).
See full target information NUP153
style
left-column

Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:3380 PubMed35697676

2022

NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda M Gleixner,Brandie Morris Verdone,Charlton G Otte,Eric N Anderson,Nandini Ramesh,Olivia R Shapiro,Jenna R Gale,Jocelyn C Mauna,Jacob R Mann,Katie E Copley,Elizabeth L Daley,Juan A Ortega,Maria Elena Cicardi,Evangelos Kiskinis,Julia Kofler,Udai B Pandey,Davide Trotti,Christopher J Donnelly

Nature cell biology 24:364-372 PubMed35292781

2022

Gasdermin E mediates resistance of pancreatic adenocarcinoma to enzymatic digestion through a YBX1-mucin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiadi Lv,Yuying Liu,Siqi Mo,Yabo Zhou,Fengye Chen,Feiran Cheng,Cong Li,Dilizhatai Saimi,Mengyu Liu,Huafeng Zhang,Ke Tang,Jingwei Ma,Zhenfeng Wang,Qiangqiang Zhu,Wei-Min Tong,Bo Huang

eLife 11: PubMed35179128

2022

Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx).

Applications

Unspecified application

Species

Unspecified reactive species

Hugo G J Damstra,Boaz Mohar,Mark Eddison,Anna Akhmanova,Lukas C Kapitein,Paul W Tillberg

The Journal of cell biology 221: PubMed34874453

2021

Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation.

Applications

Unspecified application

Species

Unspecified reactive species

Banafsheh Golchoubian,Andreas Brunner,Helena Bragulat-Teixidor,Annett Neuner,Busra A Akarlar,Nurhan Ozlu,Anne-Lore Schlaitz

iScience 24:103503 PubMed34934922

2021

Methods to separate nuclear soluble fractions reflecting localizations in living cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yutaka Ogawa,Naoko Imamoto

PLoS pathogens 17:e1009484 PubMed34543344

2021

HIV-1 requires capsid remodelling at the nuclear pore for nuclear entry and integration.

Applications

Unspecified application

Species

Unspecified reactive species

Anabel Guedán,Callum D Donaldson,Eve R Caroe,Ophélie Cosnefroy,Ian A Taylor,Kate N Bishop

Cell cycle (Georgetown, Tex.) 19:3029-3041 PubMed33054506

2020

RepoMan stimulates the chromosome-dependent pathway of microtubule assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Sofie De Munter,Gerd Van der Hoeven,Mathieu Bollen

Cells 9: PubMed32854341

2020

The Sub-Nuclear Localization of RNA-Binding Proteins in KSHV-Infected Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ella Alkalay,Chen Gam Ze Letova Refael,Irit Shoval,Noa Kinor,Ronit Sarid,Yaron Shav-Tal

The EMBO journal 39:e104467 PubMed32706158

2020

Spatial control of nucleoporin condensation by fragile X-related proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Arantxa Agote-Aran,Stephane Schmucker,Katerina Jerabkova,Inès Jmel Boyer,Alessandro Berto,Laura Pacini,Paolo Ronchi,Charlotte Kleiss,Laurent Guerard,Yannick Schwab,Hervé Moine,Jean-Louis Mandel,Sebastien Jacquemont,Claudia Bagni,Izabela Sumara

Neuron 107:1124-1140.e11 PubMed32673563

2020

GC Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa N Coyne,Benjamin L Zaepfel,Lindsey Hayes,Boris Fitchman,Yuval Salzberg,En-Ching Luo,Kelly Bowen,Hannah Trost,Stefan Aigner,Frank Rigo,Gene W Yeo,Amnon Harel,Clive N Svendsen,Dhruv Sareen,Jeffrey D Rothstein
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB81463

Anti-Nup153 antibody [R4C8]

Western blot - Anti-Nup153 antibody [R4C8] (AB81463)

  • 0
  • 0
Primary Antibodies

AB245668

Anti-Nup153 antibody

Western blot - Anti-Nup153 antibody (AB245668)

  • 0
  • 0
Primary Antibodies

AB96462

Anti-Nup153 antibody [SA1]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nup153 antibody [SA1] (AB96462)

  • 4
  • 1
Primary Antibodies

AB264554

Anti-Nup153 antibody [QE5] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [QE5] - BSA and Azide free (AB264554)

  • 0
  • 0
Primary Antibodies

AB205845

Alexa Fluor® 647 Anti-Nup153 antibody [SA1]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Nup153 antibody [SA1] (AB205845)

  • 0
  • 0
Primary Antibodies

AB204457

Alexa Fluor® 647 Anti-Nup153 antibody [QE5]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Nup153 antibody [QE5] (AB204457)

  • 5
  • 1
Primary Antibodies

AB204250

Alexa Fluor® 488 Anti-Nup153 antibody [QE5]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nup153 antibody [QE5] (AB204250)

  • 0
  • 0
Primary Antibodies

AB171074

Anti-Nup153 antibody [EPR7606(2)(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (AB171074)

  • 0
  • 0
Primary Antibodies

AB24700

Anti-Nup153 antibody [QE5]

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [QE5] (AB24700)

  • 4
  • 5

Complementary Products

Primary Antibodies

AB24609

Anti-Nuclear Pore Complex Proteins antibody [Mab414]

Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (AB24609)

  • 4
  • 12
Primary Antibodies

AB137092

Anti-NUP50 antibody [EPR9526]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (AB137092)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB256552

Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free (AB256552)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com