AB81463

Anti-Nup153 antibody [R4C8]

Name: Anti-Nup153 antibody [R4C8] (ab81463) | Abcam
Brand: Abcam Limited
SKU: AB81463
Price: 495 USD
Availability: InStock

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(3 Publications)

Rat Monoclonal Nup153 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Nup153 aa 600-1200.

View Alternative Names

Nuclear pore complex protein Nup153, 153 kDa nucleoporin, Nucleoporin Nup153, Nup153

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [R4C8] (AB81463)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [R4C8] (AB81463)

Immunofluorescent staining of HeLa cells with ab81463.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [R4C8] (AB81463)

Immunofluorescent staining of rat neuron with ab81463. The dots are Nup153.

Unknown

Western blot - Anti-Nup153 antibody [R4C8] (AB81463)

All lanes:

Western blot - Anti-Nup153 antibody [R4C8] (ab81463)

Lane 1:

Nuclear membrane fraction from HeLa cells

Lane 2:

Nuclear membrane fraction from NIH3T3 cells

Predicted band size: 154 kDa

Observed band size: 160 kDa

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Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

R4C8

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Rat Nup153 aa 600-1200. The exact immunogen used to generate this antibody is proprietary information.

P49791

Epitope

Amino acids 610-1191 (Zn finger and FG repeats domain).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Purification notes

This antibody was purified from the serum free cultured medium of the hybridoma under mild conditions by propriety chromatography processes. It is filter sterilized.

Storage buffer

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nup153 also known as nucleoporin 153 is a component of the nuclear pore complex (NPC) with a molecular weight of approximately 153 kDa. It functions as a scaffold within the NPC which mediates the bidirectional transport of molecules between the nucleus and the cytoplasm. Nup153 is part of the FG-nucleoporin family characterized by domains rich in phenylalanine and glycine repeats. It is highly expressed in the nuclear envelope of eukaryotic cells playing a vital part in maintaining the permeability of the nuclear envelope.

Biological function summary

Nup153 contributes to the assembly and disassembly of the NPC during cell cycle progression. It acts as a docking site for various nuclear import and export receptors therefore is essential for nucleocytoplasmic transport. Nup153 is an important component in the larger nuclear pore complex which itself is made up of multiple nucleoporins forming a sophisticated structure capable of both passive and active transport processes.

Pathways

Nup153 is intricately involved in the nucleocytoplasmic transport pathway and plays a critical role in RNA processing. It interacts with proteins such as Importin β and Exportin which facilitate the transport of different molecules across the nuclear envelope. This pathway is fundamental for the proper regulation of gene expression and cell cycle control exhibiting the importance of Nup153 in cellular dynamics.

Nup153 has been linked to cancer and neurodegenerative diseases. Alterations in Nup153 expression or function can disrupt cellular homeostasis leading to uncontrolled cell proliferation or impaired neuronal function. In cancer it may show altered interactions with p53 an important tumor suppressor protein affecting its regulatory functions. Understanding the role of Nup153 in such diseases may offer insights into potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Mediates TPR anchoring to the nuclear membrane at NPC (By similarity). The repeat-containing domain may be involved in anchoring other components of the NPC to the pore membrane. Possible DNA-binding subunit of the nuclear pore complex (NPC) (By similarity).

See full target information Nup153

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Genome medicine 17:102 PubMed40999499

2025

EMB is essential for enteric nervous system development mediated by PI3K signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Li,Didi Zhuansun,Xinyao Meng,Heying Yang,Jun Xiao,Yingjian Chen,Jing Wang,Xiaosi Yu,Zejian Li,Jingyi You,Xuyong Chen,Chenzhao Feng,Luyao Wu,Xufeng Chu,Weicheng Duan,Kang Wang,Zongzhe Li,Jinfa Tou,Lei Yu,Weibing Tang,Yuanmei Liu,Xuewu Jiang,Hongxia Ren,Mei Yu,Qiang Yin,Xiang Liu,Zhilin Xu,Dianming Wu,Chunlei Jiao,Donghai Yu,Xiaojuan Wu,Tianqi Zhu,Jixin Yang,Lei Xiang,Jing Wang,Qiong Wang,Bingyan Zhou,Di Wang,Ke Chen,Handan Mao,Bin Wang,Jianghua Zhan,Cong-Yi Wang,Wanjiang Zeng,Feng Chen,Bo Xiong,Jiexiong Feng

Cells 10: PubMed34063931

2021

Nucleoporin TPR Affects C2C12 Myogenic Differentiation via Regulation of Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jana Uhlířová,Lenka Šebestová,Karel Fišer,Tomáš Sieger,Jindřiška Fišerová,Pavel Hozák

The Journal of cell biology 220: PubMed33306092

2020

An APEX2 proximity ligation method for mapping interactions with the nuclear lamina.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph R Tran,Danielle I Paulson,James J Moresco,Stephen A Adam,John R Yates,Robert D Goldman,Yixian Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nup153 antibody [R4C8]

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [R4C8] (AB81463)

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [R4C8] (AB81463)

Western blot - Anti-Nup153 antibody [R4C8] (AB81463)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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