AB157104

Anti-NUP155 antibody

Name: Anti-NUP155 antibody (ab157104) | Abcam
Brand: Abcam Limited
SKU: AB157104

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(3 Publications)

Rabbit Polyclonal NUP155 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NUP155 aa 1300 to C-terminus.

View Alternative Names

KIAA0791, NUP155, Nuclear pore complex protein Nup155, 155 kDa nucleoporin, Nucleoporin Nup155

2 Images

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Immunoprecipitation - Anti-NUP155 antibody (AB157104)

Detection of NUP155 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157104 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab157104 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-NUP155 antibody (ab157104)

Predicted band size: 155 kDa

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Unknown

Western blot - Anti-NUP155 antibody (AB157104)

All lanes:

Western blot - Anti-NUP155 antibody (ab157104) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 155 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human NUP155 aa 1300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75694

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab157104 was affinity purified using an epitope specific to NUP155 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUP155 is a nucleoporin a component of the nuclear pore complex that regulates nucleocytoplasmic transport. Its mass is approximately 155 kDa. The protein is prominently expressed in tissues with high cellular activity like the liver and pancreas as it assists in managing the transport needs of these metabolically active areas. NUP155 facilitates the exchange of molecules between the nucleus and the cytoplasm playing a significant role in cellular communication.

Biological function summary

This nucleoporin is essential in maintaining the structure and function of the nuclear envelope. NUP155 is part of the extensive nuclear pore complex which consists of multiple proteins working together to manage the transport of RNA and proteins across the nuclear envelope. NUP155 acts as a scaffold that helps maintain the integrity of this complex ensuring the proper exchange between nuclear and cytoplasmic compartments vital for gene regulation and expression.

Pathways

NUP155 participates in pathways like nucleocytoplasmic transport and cell cycle regulation. It interacts with other nuclear pore proteins such as NUP93 and NUP205 to coordinate the import and export of macromolecules. In the cell cycle pathway NUP155 ensures proper mitotic progression by managing the transport of regulatory proteins necessary for cell division.

Alterations in NUP155 function have associations with cardiac arrhythmias and certain types of cancers. In arrhythmias NUP155 mutations disrupt cellular signaling processes affecting cardiac rhythm regulation. Linked with tumorigenesis abnormal NUP155 expression can alter nucleocytoplasmic transport contributing to cancer development. Its interactions with other proteins like NUP93 may offer insights into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Essential component of nuclear pore complex. Could be essessential for embryogenesis. Nucleoporins may be involved both in binding and translocating proteins during nucleocytoplasmic transport.

See full target information NUP155

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 37:e23383 PubMed37158446

2023

Knockdown of circMFN2 inhibits cell progression and glycolysis by miR-198/CUL4B pathway in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Song,Ting Chai,Junqi Liu,Alan Chu,Chen Sun,Zongwen Liu

Frontiers in oncology 10:162 PubMed32133296

2020

Validation of Novel Prognostic Biomarkers for Early-Stage Clear-Cell, Endometrioid and Mucinous Ovarian Carcinomas Using Immunohistochemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna Engqvist,Toshima Z Parris,Anikó Kovács,Elisabeth Werner Rönnerman,Karin Sundfeldt,Per Karlsson,Khalil Helou

eLife 7: PubMed30084827

2018

Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa Kane,Stephanie V Rebensburg,Matthew A Takata,Trinity M Zang,Masahiro Yamashita,Mamuka Kvaratskhelia,Paul D Bieniasz

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NUP155 antibody

Immunoprecipitation - Anti-NUP155 antibody (AB157104)

Western blot - Anti-NUP155 antibody (AB157104)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise