AB251277

Anti-NUP155 antibody [EPR17111] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal NUP155 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0791, NUP155, Nuclear pore complex protein Nup155, 155 kDa nucleoporin, Nucleoporin Nup155

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-NUP155 antibody [EPR17111] - BSA and Azide free (AB251277)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUP155 antibody [EPR17111] - BSA and Azide free (AB251277)

This data was developed using ab199528, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NUP155 with ab199528 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nucleus membrane and cytoplasm staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows : -
-ve control 1 : ab199528 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Flow Cytometry (Intracellular) - Anti-NUP155 antibody [EPR17111] - BSA and Azide free (AB251277)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NUP155 antibody [EPR17111] - BSA and Azide free (AB251277)

This data was developed using ab199528, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NUP155 with ab199528 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Supplier Data

Western blot - Anti-NUP155 antibody [EPR17111] - BSA and Azide free (AB251277)

This data was developed using ab199528, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NUP155 antibody [EPR17111] (<a href='/en-us/products/primary-antibodies/nup155-antibody-epr17111-ab199528'>ab199528</a>) at 1/2000 dilution

All lanes:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 155 kDa

Observed band size: 155 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-NUP155 antibody [EPR17111] - BSA and Azide free (AB251277)

This data was developed using ab199528, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NUP155 antibody [EPR17111] (<a href='/en-us/products/primary-antibodies/nup155-antibody-epr17111-ab199528'>ab199528</a>) at 1/1000 dilution

All lanes:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 155 kDa

Observed band size: 155 kDa

false

Exposure time: 3min

Unknown

Western blot - Anti-NUP155 antibody [EPR17111] - BSA and Azide free (AB251277)

This data was developed using ab199528, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NUP155 antibody [EPR17111] (<a href='/en-us/products/primary-antibodies/nup155-antibody-epr17111-ab199528'>ab199528</a>) at 1/5000 dilution

Lane 1:

Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 10 µg

Lane 2:

HEK-293 (Human embryonic kidney) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 155 kDa

Observed band size: 155 kDa

false

Exposure time: 30s

Unconjugated

Anti-NUP155 antibody [EPR17111]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17111

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab251277 is the carrier-free version of ab199528.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUP155 is a nucleoporin a component of the nuclear pore complex that regulates nucleocytoplasmic transport. Its mass is approximately 155 kDa. The protein is prominently expressed in tissues with high cellular activity like the liver and pancreas as it assists in managing the transport needs of these metabolically active areas. NUP155 facilitates the exchange of molecules between the nucleus and the cytoplasm playing a significant role in cellular communication.

Biological function summary

This nucleoporin is essential in maintaining the structure and function of the nuclear envelope. NUP155 is part of the extensive nuclear pore complex which consists of multiple proteins working together to manage the transport of RNA and proteins across the nuclear envelope. NUP155 acts as a scaffold that helps maintain the integrity of this complex ensuring the proper exchange between nuclear and cytoplasmic compartments vital for gene regulation and expression.

Pathways

NUP155 participates in pathways like nucleocytoplasmic transport and cell cycle regulation. It interacts with other nuclear pore proteins such as NUP93 and NUP205 to coordinate the import and export of macromolecules. In the cell cycle pathway NUP155 ensures proper mitotic progression by managing the transport of regulatory proteins necessary for cell division.

Alterations in NUP155 function have associations with cardiac arrhythmias and certain types of cancers. In arrhythmias NUP155 mutations disrupt cellular signaling processes affecting cardiac rhythm regulation. Linked with tumorigenesis abnormal NUP155 expression can alter nucleocytoplasmic transport contributing to cancer development. Its interactions with other proteins like NUP93 may offer insights into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of nuclear pore complex. Could be essessential for embryogenesis. Nucleoporins may be involved both in binding and translocating proteins during nucleocytoplasmic transport.

See full target information NUP155

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed38732558

2024

Differential Modulation by Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) of Mesenteric Fat and Macrophages and T Cells in Adipose Tissue of Obese / Zucker Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Hong,Peter Zahradka,Carla G Taylor

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUP155 antibody [EPR17111] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-NUP155 antibody [EPR17111] - BSA and Azide free (AB251277)

Flow Cytometry (Intracellular) - Anti-NUP155 antibody [EPR17111] - BSA and Azide free (AB251277)

Western blot - Anti-NUP155 antibody [EPR17111] - BSA and Azide free (AB251277)

Western blot - Anti-NUP155 antibody [EPR17111] - BSA and Azide free (AB251277)

Western blot - Anti-NUP155 antibody [EPR17111] - BSA and Azide free (AB251277)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise