AB157090

Anti-NUP205 antibody

Name: Anti-NUP205 antibody (ab157090) | Abcam
Brand: Abcam Limited
SKU: AB157090
Price: 510 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal NUP205 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NUP205 aa 1750-1800.

View Alternative Names

C7orf14, KIAA0225, NUP205, Nuclear pore complex protein Nup205, 205 kDa nucleoporin, Nucleoporin Nup205

2 Images

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Immunoprecipitation - Anti-NUP205 antibody (AB157090)

ab157090 at 1 µg/ml detecting NUP205 in 293T whole cell lysate by WB following IP.

Lane 1 : IP with an antibody which recognizes NUP205
Lane 2 : ab157090 at 6 µg/mg of lysate
Lane 3 : Control IgG.

In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-NUP205 antibody (ab157090)

Predicted band size: 228 kDa

false

Unknown

Western blot - Anti-NUP205 antibody (AB157090)

All lanes:

Western blot - Anti-NUP205 antibody (ab157090) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 228 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human NUP205 aa 1750-1800. The exact immunogen used to generate this antibody is proprietary information.

Q92621

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Primates": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab157090 was affinity purified using an epitope specific to NUP205 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein NUP205 also known as nucleoporin 205 kDa is an important structural component of the nuclear pore complex (NPC). It has a molecular mass of approximately 205 kDa. NUP205 is expressed ubiquitously in eukaryotic cells as part of the nuclear envelope where it is an essential link in regulating the transport of molecules between the nucleus and the cytoplasm. The expression patterns show its presence across different cell types indicating its fundamental role in cellular function.

Biological function summary

NUP205 is an essential component of the nuclear pore complex contributing to nuclear-cytoplasmic transport. The protein works in concert with other nucleoporins to form the scaffold of the NPC facilitating the bidirectional movement of proteins and RNA. NUP205 as part of this large multiprotein complex plays a significant role in maintaining cellular compartmentalization and influencing gene expression patterns by controlling the transit of regulatory molecules in and out of the nucleus.

Pathways

NUP205 interacts significantly within the nuclear transport pathway. It engages intimately in the regulation of protein and RNA shuttling which influences gene expression and cell cycle processes. NUP205 associates with other nucleoporins such as NUP214 and NUP62 participating in processes like signal transduction and RNA processing. Its functionality supports broader cellular pathways by acting as a gatekeeper ensuring essential factors access or exit the nucleus as needed.

NUP205 has connections to various pathological conditions stemming from disrupted nucleocytoplasmic transport. It is implicated in acute myeloid leukemia (AML) where aberrant expression or mutations of NUP205 can disrupt normal cellular transport functions possibly in association with NUP214. Additionally defects in NUP205 function have been linked to certain neurodegenerative conditions highlighting its role in maintaining neuronal cell homeostasis. Understanding NUP205's molecular behavior offers potential insights into the pathogenesis of these diseases and targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance (PubMed : 9348540). May anchor NUP62 and other nucleoporins, but not NUP153 and TPR, to the NPC (PubMed : 15229283). In association with TMEM209, may be involved in nuclear transport of various nuclear proteins in addition to MYC (PubMed : 22719065).

See full target information NUP205

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 15:338 PubMed39115575

2024

A metabolic reprogramming-related gene signature correlates with prognosis and proliferation of BLCA.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoxin Wu,Yi Luo,Tinghao Li

Nature 613:575-581 PubMed36599981

2023

A quantitative map of nuclear pore assembly reveals two distinct mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Shotaro Otsuka,Jeremy O B Tempkin,Wanlu Zhang,Antonio Z Politi,Arina Rybina,M Julius Hossain,Moritz Kueblbeck,Andrea Callegari,Birgit Koch,Natalia Rosalia Morero,Andrej Sali,Jan Ellenberg

eLife 7: PubMed30084827

2018

Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa Kane,Stephanie V Rebensburg,Matthew A Takata,Trinity M Zang,Masahiro Yamashita,Mamuka Kvaratskhelia,Paul D Bieniasz

Cell 173:958-971.e17 PubMed29628143

2018

Stress Granule Assembly Disrupts Nucleocytoplasmic Transport.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Zhang,J Gavin Daigle,Kathleen M Cunningham,Alyssa N Coyne,Kai Ruan,Jonathan C Grima,Kelly E Bowen,Harsh Wadhwa,Peiguo Yang,Frank Rigo,J Paul Taylor,Aaron D Gitler,Jeffrey D Rothstein,Thomas E Lloyd

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUP205 antibody

Immunoprecipitation - Anti-NUP205 antibody (AB157090)

Western blot - Anti-NUP205 antibody (AB157090)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise