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AB70497

Anti-NUP214 antibody

4

(4 Reviews)

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(23 Publications)

Rabbit Polyclonal NUP214 antibody. Suitable for IHC-P, ICC, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human NUP214.

View Alternative Names

CAIN, CAN, KIAA0023, NUP214, Nuclear pore complex protein Nup214, 214 kDa nucleoporin, Nucleoporin Nup214, Protein CAN

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP214 antibody (AB70497)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP214 antibody (AB70497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma labeling NUP214 with ab70497 at 1/200 dilution.

Detection : DAB

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP214 antibody (AB70497)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP214 antibody (AB70497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma labeling NUP214 with ab70497 at 1/200 dilution.

Detection : DAB

Immunoprecipitation - Anti-NUP214 antibody (AB70497)
  • IP

Supplier Data

Immunoprecipitation - Anti-NUP214 antibody (AB70497)

NUP214 was immunoprecipitated from HeLa whole cell lysate (0.1 mg per IP reaction, 20% loaded) with ab70497 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab70497 at 0.04 μg/mL.

Lane 1 : ab70497 IP in HeLa whole cell lysate.

Lane 2 : ab70497 IP in HeLa whole cell lysate.

Lane 2 : rabbit anti-NUP214 antibody IP in HeLa whole cell lysate.

Lane 4 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-NUP214 antibody (ab70497)

Predicted band size: 214 kDa

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Western blot - Anti-NUP214 antibody (AB70497)
  • WB

Supplier Data

Western blot - Anti-NUP214 antibody (AB70497)

All lanes:

Western blot - Anti-NUP214 antibody (ab70497) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK293T whole cell lysate at 50 µg

Predicted band size: 214 kDa

false

Western blot - Anti-NUP214 antibody (AB70497)
  • WB

CiteAb

Western blot - Anti-NUP214 antibody (AB70497)

NUP214 western blot using anti-NUP214 antibody ab70497. Publication image and figure legend from Bonnin, E., Cabochette, P., et al., 2018, PLoS Genet, PubMed 30543681.

ab70497 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab70497 please see the product overview.

NUP88 mutants have distinct effects on NUP88 nucleoporin partners.(A) GFP-trap assays to study the effects of NUP88 mutants on its interaction with NUP214, NUP62 and NUP98. GFP and GFP-NUP88 fusion proteins were transiently expressed in HeLa cells. After 48 h, GFP proteins and associated factors were recovered from cell lysates and probed by Western blot analysis using antibodies against Nup214, NUP62, and NUP98. Successful transfection and expression of the proteins was confirmed by probing with antibodies against GFP. GFP proteins had the following size : GFP-NUP88 WT, D434Y, and E634del : ~115 (88 + 27) kDa; GFP-NUP88 R509* : 83 (56 + 27) kDa; GFP : 27 kDa. (B-D) HeLa cells were transiently transfected with GFP-constructs of the respective NUP88 mutant and fixed and stained after 48 h for immunofluorescence microscopy using (B) anti-NUP214, (C) anti-NUP62, and (D) anti-NUP98 antibodies. Only NUP88 E634del affects NUP62 association with NPCs. Scale bars : 10 μm.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, IHC-P, IP, ICC/IF, ICC

applications

Immunogen

Synthetic Peptide within Human NUP214. The exact immunogen used to generate this antibody is proprietary information.

P35658

Specificity

We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The nucleoporin 214 (NUP214) also known as CAN is an essential component of the nuclear pore complex (NPC) which facilitates the transport of molecules between the nucleus and the cytoplasm. NUP214 has a molecular mass of approximately 214 kDa a characteristic that partially defines its naming. It expresses widely in various cell types reflecting its fundamental role in cellular transport processes. Within the NPC NUP214 partners with other nucleoporins to form critical subunits that ensure efficient nucleocytoplasmic transport.
Biological function summary

NUP214 participates in the assembly and function of the NPC by regulating cargo movement. This protein acts as a docking site for transport receptors aiding in the import and export of macromolecules. NUP214 exhibits a part in the nuclear pore complex interacting dynamically with other nucleoporins like NUP88 and NUP62. Its biological role maintains nuclear and cytoplasmic homeostasis which is important for normal cell function and division influencing gene expression and cellular responses to stimuli.

Pathways

NUP214 plays a significant role in nucleocytoplasmic transport mechanisms and is integrated into the Ran GTPase pathway. This pathway ensures that proteins and RNA efficiently move across the nuclear envelope fulfilling specific cellular functions. NUP214's functional relationships include interactions with importin and exportin proteins which recognize nuclear localization and export signals on cargo molecules. These interactions are vital for the cell cycle and signal transduction pathways highlighting NUP214's involvement in important cellular activities.

NUP214 often associates with certain forms of leukemia such as acute myeloid leukemia (AML). Aberrations in NUP214 like translocations or amplifications can lead to malignancies through disrupted nucleocytoplasmic transport and abnormal gene expression. The fusion protein generated from NUP214-ABL1 resulting from a chromosomal translocation directly involves in leukemogenesis by activating abnormal signaling pathways contributing to oncogenesis. Understanding NUP214's role in these disorders provides insight into potential therapeutic targets for treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the nuclear pore complex (PubMed : 9049309). Has a critical role in nucleocytoplasmic transport (PubMed : 31178128). May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex (PubMed : 31178128, PubMed : 8108440).. (Microbial infection) Required for capsid disassembly of the human adenovirus 5 (HadV-5) leading to release of the viral genome to the nucleus (in vitro).
See full target information NUP214
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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6548 PubMed40670348

2025

Elaboration of molecular glues that target TRIM21 into TRIMTACs that degrade protein aggregates.

Applications

Unspecified application

Species

Unspecified reactive species

Marc A Scemama de Gialluly,Anthony R Allen,Elijah H Hayes,Patrick Zhuang,Ralston B Goldfarb,Amanda N Farrar,Yuriy Fedorov,Drew J Adams

EMBO reports 25:4226-4251 PubMed39026009

2024

Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Misha Kalarikkal,Rimpi Saikia,Lizanne Oliveira,Yashashree Bhorkar,Akshay Lonare,Pallavi Varshney,Prathamesh Dhamale,Amitabha Majumdar,Jomon Joseph

PLoS pathogens 20:e1011640 PubMed38215165

2024

Murine leukemia virus infection of non-dividing dendritic cells is dependent on nucleoporins.

Applications

Unspecified application

Species

Unspecified reactive species

Karen Salas-Briceno,Wenming Zhao,Susan R Ross

The Journal of cell biology 222: PubMed36920247

2023

A general method for quantitative fractionation of mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yael Udi,Wenzhu Zhang,Milana E Stein,Inna Ricardo-Lax,Hilda A Pasolli,Brian T Chait,Michael P Rout

Nature communications 13:5881 PubMed36202822

2022

RNA export through the nuclear pore complex is directional.

Applications

Unspecified application

Species

Unspecified reactive species

Asaf Ashkenazy-Titelman,Mohammad Khaled Atrash,Alon Boocholez,Noa Kinor,Yaron Shav-Tal

Genes to cells : devoted to molecular & cellular mechanisms 26:830-837 PubMed34320268

2021

SET-NUP214 and MLL cooperatively regulate the promoter activity of the HoxA10 gene.

Applications

Unspecified application

Species

Unspecified reactive species

Sadik Cigdem,Shoko Saito,Daiki Nishikata,Kyosuke Nagata,Mitsuru Okuwaki

Turkish journal of biology = Turk biyoloji dergisi 44:82-92 PubMed32256144

2020

Interaction of influenza A virus NS2/NEP protein with the amino-terminal part of Nup214.

Applications

Unspecified application

Species

Unspecified reactive species

Burçak Şenbaş Akyazi,Ayşegül Pirinçal,Atsushi Kawaguchi,Kyosuke Nagata,Kadir Turan

Frontiers in oncology 9:1383 PubMed31921641

2019

Long Non-coding RNA Facilitates Colorectal Cancer Development Through EZH2/DNMT1-Induced miR-133b Suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Lv Lv,Liang He,Shaohua Chen,Yaqun Yu,Guosong Che,Xuan Tao,Shengtao Wang,Zhiyuan Jian,Xuemei Zhang

The Journal of cell biology 218:2962-2981 PubMed31375530

2019

Imaging within single NPCs reveals NXF1's role in mRNA export on the cytoplasmic side of the pore.

Applications

Unspecified application

Species

Unspecified reactive species

Rakefet Ben-Yishay,Amir Mor,Amit Shraga,Asaf Ashkenazy-Titelman,Noa Kinor,Avital Schwed-Gross,Avi Jacob,Noga Kozer,Pramod Kumar,Yuval Garini,Yaron Shav-Tal

American journal of human genetics 105:48-64 PubMed31178128

2019

Pathogenic Variants in NUP214 Cause "Plugged" Nuclear Pore Channels and Acute Febrile Encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Boris Fichtman,Tamar Harel,Nitzan Biran,Fadia Zagairy,Carolyn D Applegate,Yuval Salzberg,Tal Gilboa,Somaya Salah,Avraham Shaag,Natalia Simanovsky,Houriya Ayoubieh,Nara Sobreira,Giuseppe Punzi,Ciro Leonardo Pierri,Ada Hamosh,Orly Elpeleg,Amnon Harel,Simon Edvardson
View all publications
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