Rabbit Polyclonal NUP35 antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NUP35.
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Publications
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- Nature communications 6:88822015Combining Spinach-tagged RNA and gene localization to image gene expression in live yeast.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Guet et. al.PubMed 26582123
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: Whole serum- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human NUP35. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NFH5
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Saccharomyces cerevisiae | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC.
Alternative names
MP44, NUP53, NUP35, Nucleoporin NUP35, 35 kDa nucleoporin, Mitotic phosphoprotein 44, Nuclear pore complex protein Nup53, Nucleoporin NUP53, MP-44
Recommended products
Rabbit Polyclonal NUP35 antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NUP35.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: Whole serum- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human NUP35. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NFH5
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NUP35 also known as Nucleoporin 35 kDa is a nuclear pore complex protein with a mass of about 35 kDa. It plays an important mechanical role in regulating the transport of molecules between the nucleus and the cytoplasm. NUP35 is expressed ubiquitously in eukaryotic cells. It takes part in forming the nuclear pore complex structure which acts as a gateway for nucleocytoplasmic transport.
- Biological function summary
NUP35 is involved in the composition of the nuclear pore complex (NPC) essential for cellular function. As a component of NPC NUP35 interacts with other nucleoporins to help maintain the NPC's architecture and stability. This structure allows selective molecular trafficking like RNA export and import of proteins supporting essential cellular processes. NUP35 contributes to maintaining the dynamic barrier and transport of molecules ensuring correct cellular homeostasis.
- Pathways
NUP35's role in nucleocytoplasmic transport has implications in pathways like RNA transport and gene expression regulation. It interacts closely with other nucleoporins within the nuclear pore complex. The NPC itself intersects with key pathways responsible for mRNA export from the nucleus to the cytoplasm primarily interacting with proteins such as NUP98 and NUP62 which play roles in facilitating efficient transport processes.
- Associated diseases and disorders
Faulty NUP35 function relates to diseases like cancer and neurodegenerative disorders. Aberrations in its function can lead to altered nucleocytoplasmic transport dynamics potentially causing cellular proliferation or apoptosis. The improper regulation can further influence other related proteins such as NUP214 which has been implicated in leukemia highlighting the importance of normal NUP35 function in health and disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-NUP35 antibody (ab11692)
ab11692 at a 1/1000 dilution staining approximately 53kDa Nup35 in S.cerevisiae nuclear and whole cell lysate by Western blot (ECL). ab11692 at a 1/1000 dilution staining approximately 53kDa Nup35 in S.cerevisiae nuclear and whole cell lysate by Western blot (ECL).
All lanes: Western blot - Anti-NUP35 antibody (ab11692)
Predicted band size: 35 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NUP35 antibody (ab11692)
ab11692 at a 1/1000 dilution staining Nup35 in S.cerevisiae by immunofluorescence.
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