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AB11692

Anti-NUP35 antibody

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(1 Publication)

Rabbit Polyclonal NUP35 antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NUP35.

View Alternative Names

MP44, NUP53, NUP35, Nucleoporin NUP35, 35 kDa nucleoporin, Mitotic phosphoprotein 44, Nuclear pore complex protein Nup53, Nucleoporin NUP53, MP-44

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-NUP35 antibody (AB11692)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUP35 antibody (AB11692)

ab11692 at a 1/1000 dilution staining Nup35 in S.cerevisiae by immunofluorescence.

Western blot - Anti-NUP35 antibody (AB11692)
  • WB

Unknown

Western blot - Anti-NUP35 antibody (AB11692)

ab11692 at a 1/1000 dilution staining approximately 53kDa Nup35 in S.cerevisiae nuclear and whole cell lysate by Western blot (ECL). ab11692 at a 1/1000 dilution staining approximately 53kDa Nup35 in S.cerevisiae nuclear and whole cell lysate by Western blot (ECL).

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Western blot - Anti-NUP35 antibody (ab11692)

Predicted band size: 35 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NUP35. The exact immunogen used to generate this antibody is proprietary information.

Q8NFH5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Preservative: 0.1% Sodium azide Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUP35 also known as Nucleoporin 35 kDa is a nuclear pore complex protein with a mass of about 35 kDa. It plays an important mechanical role in regulating the transport of molecules between the nucleus and the cytoplasm. NUP35 is expressed ubiquitously in eukaryotic cells. It takes part in forming the nuclear pore complex structure which acts as a gateway for nucleocytoplasmic transport.
Biological function summary

NUP35 is involved in the composition of the nuclear pore complex (NPC) essential for cellular function. As a component of NPC NUP35 interacts with other nucleoporins to help maintain the NPC's architecture and stability. This structure allows selective molecular trafficking like RNA export and import of proteins supporting essential cellular processes. NUP35 contributes to maintaining the dynamic barrier and transport of molecules ensuring correct cellular homeostasis.

Pathways

NUP35's role in nucleocytoplasmic transport has implications in pathways like RNA transport and gene expression regulation. It interacts closely with other nucleoporins within the nuclear pore complex. The NPC itself intersects with key pathways responsible for mRNA export from the nucleus to the cytoplasm primarily interacting with proteins such as NUP98 and NUP62 which play roles in facilitating efficient transport processes.

Faulty NUP35 function relates to diseases like cancer and neurodegenerative disorders. Aberrations in its function can lead to altered nucleocytoplasmic transport dynamics potentially causing cellular proliferation or apoptosis. The improper regulation can further influence other related proteins such as NUP214 which has been implicated in leukemia highlighting the importance of normal NUP35 function in health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC.
See full target information NUP35
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 6:8882 PubMed26582123

2015

Combining Spinach-tagged RNA and gene localization to image gene expression in live yeast.

Applications

Unspecified application

Species

Unspecified reactive species

David Guet,Laura T Burns,Suman Maji,Jérôme Boulanger,Pascal Hersen,Susan R Wente,Jean Salamero,Catherine Dargemont
View all publications
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Product promise

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