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Mouse Polyclonal NUP43 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Nucleoporin Nup43.

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Images

Western blot - Anti-NUP43 antibody (AB69447), expandable thumbnail
  • Western blot - Anti-NUP43 antibody (AB69447), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NUP43 antibody (AB69447), expandable thumbnail

Key facts

Isotype

IgG

Host species

Mouse

Storage buffer

pH: 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human Nucleoporin Nup43. Database link Q8NFH3

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/500 - 1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

10 µg/mL

Notes

-

Target data

Function

Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation.

Alternative names

Recommended products

Mouse Polyclonal NUP43 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Nucleoporin Nup43.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human Nucleoporin Nup43. Database link Q8NFH3
Carrier free

Yes

Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NUP43 also known as nucleoporin 43 is an important component of the nuclear pore complex (NPC). Weighing approximately 43 kDa this protein is expressed in the nuclear envelope of eukaryotic cells. NUP43 is part of the Y-complex which plays a mechanical role in mediating the transport of molecules between the nucleus and the cytoplasm. Its presence in the nuclear pore structure facilitates important cellular functions including regulation and signaling.

Biological function summary

The organization and function of the nuclear pore complex involve NUP43 integration. It forms part of a larger supramolecular structure assisting in functions alongside other nucleoporins. Inside the NPC NUP43 contributes to nuclear-cytoplasmic transport by interacting with transport receptors and cargo molecules. This interaction regulates the entry and exit of macromolecules maintaining cellular homeostasis and gene expression processes.

Pathways

NUP43 integrates cellular pathways related to nucleocytoplasmic transport and mRNA processing. It functions in tandem with other nucleoporins within the NPC such as NUP107 to control the flow of genetic information and proteins. The precise regulation through these pathways ensures cellular integrity and proper gene expression affecting a range of cellular activities essential for cell survival and adaptation.

Associated diseases and disorders

Abnormal function or expression of NUP43 links to disorders like cancer and neurodegenerative diseases. It often associates with other nucleoporins such as NUP133 in pathologies where nuclear transport is disrupted. Understanding the role of NUP43 in these diseases contributes to insights into potential therapeutic targets which may help in the development of interventions to modulate its activity or interactions in the disease context.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Western blot - Anti-NUP43 antibody (ab69447), expandable thumbnail

    Western blot - Anti-NUP43 antibody (ab69447)

    All lanes: Western blot - Anti-NUP43 antibody (ab69447) at 1/500 dilution

    All lanes: human placenta tissue lysate at 25 µg

    Secondary

    All lanes: Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size: 42 kDa

    Observed band size: 43 kDa, 60 kDa

  • Western blot - Anti-NUP43 antibody (ab69447), expandable thumbnail

    Western blot - Anti-NUP43 antibody (ab69447)

    All lanes: Western blot - Anti-NUP43 antibody (ab69447) at 1/500 dilution

    Lane 1: NUP43 transfected 293T lysate at 25 µg

    Lane 2: Non transfected 293T lysate at 25 µg

    Secondary

    All lanes: Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size: 42 kDa

    Observed band size: 43 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP43 antibody (ab69447), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NUP43 antibody (ab69447)

    ab69447 at 10μg/ml staining NUP43 in Hela cells by ICC/IF.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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