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AB69447

Anti-NUP43 antibody

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(2 Publications)

Mouse Polyclonal NUP43 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Nucleoporin Nup43.

View Alternative Names

Nucleoporin Nup43, Nup107-160 subcomplex subunit Nup43, p42, NUP43

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-NUP43 antibody (AB69447)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUP43 antibody (AB69447)

ab69447 at 10μg/ml staining NUP43 in Hela cells by ICC/IF.

Western blot - Anti-NUP43 antibody (AB69447)
  • WB

Unknown

Western blot - Anti-NUP43 antibody (AB69447)

All lanes:

Western blot - Anti-NUP43 antibody (ab69447) at 1/500 dilution

All lanes:

human placenta tissue lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 42 kDa

Observed band size: 43 kDa,60 kDa

false

Western blot - Anti-NUP43 antibody (AB69447)
  • WB

Unknown

Western blot - Anti-NUP43 antibody (AB69447)

All lanes:

Western blot - Anti-NUP43 antibody (ab69447) at 1/500 dilution

Lane 1:

NUP43 transfected 293T lysate at 25 µg

Lane 2:

Non transfected 293T lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 42 kDa

Observed band size: 43 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Nucleoporin Nup43.

Q8NFH3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUP43 also known as nucleoporin 43 is an important component of the nuclear pore complex (NPC). Weighing approximately 43 kDa this protein is expressed in the nuclear envelope of eukaryotic cells. NUP43 is part of the Y-complex which plays a mechanical role in mediating the transport of molecules between the nucleus and the cytoplasm. Its presence in the nuclear pore structure facilitates important cellular functions including regulation and signaling.
Biological function summary

The organization and function of the nuclear pore complex involve NUP43 integration. It forms part of a larger supramolecular structure assisting in functions alongside other nucleoporins. Inside the NPC NUP43 contributes to nuclear-cytoplasmic transport by interacting with transport receptors and cargo molecules. This interaction regulates the entry and exit of macromolecules maintaining cellular homeostasis and gene expression processes.

Pathways

NUP43 integrates cellular pathways related to nucleocytoplasmic transport and mRNA processing. It functions in tandem with other nucleoporins within the NPC such as NUP107 to control the flow of genetic information and proteins. The precise regulation through these pathways ensures cellular integrity and proper gene expression affecting a range of cellular activities essential for cell survival and adaptation.

Abnormal function or expression of NUP43 links to disorders like cancer and neurodegenerative diseases. It often associates with other nucleoporins such as NUP133 in pathologies where nuclear transport is disrupted. Understanding the role of NUP43 in these diseases contributes to insights into potential therapeutic targets which may help in the development of interventions to modulate its activity or interactions in the disease context.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation.
See full target information Nucleoporin Nup43
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Neuron 107:1124-1140.e11 PubMed32673563

2020

GC Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa N Coyne,Benjamin L Zaepfel,Lindsey Hayes,Boris Fitchman,Yuval Salzberg,En-Ching Luo,Kelly Bowen,Hannah Trost,Stefan Aigner,Frank Rigo,Gene W Yeo,Amnon Harel,Clive N Svendsen,Dhruv Sareen,Jeffrey D Rothstein

Frontiers in cell and developmental biology 8:579 PubMed32733888

2020

Glucose Availability Alters Gene and Protein Expression of Several Newly Classified and Putative Solute Carriers in Mice Cortex Cell Culture and .

Applications

Unspecified application

Species

Unspecified reactive species

Mikaela M Ceder,Emilia Lekholm,Axel Klaesson,Rekha Tripathi,Nadine Schweizer,Lydia Weldai,Sourabh Patil,Robert Fredriksson
View all publications
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Product promise

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