AB151567

Anti-NUP50 antibody [EPR9525]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal NUP50 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

View Alternative Names

NPAP60L, PRO1146, NUP50, Nuclear pore complex protein Nup50, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, Nucleoporin Nup50

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9525] (AB151567)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9525] (AB151567)

Immunofluorescent analysis of HeLa cells labeling NUP50 with ab151567 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (AB151567)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (AB151567)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling NUP50 with ab151567 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (AB151567)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (AB151567)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (AB151567)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (AB151567)

Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (AB151567)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (AB151567)

Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (AB151567)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (AB151567)

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (AB151567)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (AB151567)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-NUP50 antibody [EPR9525] (AB151567)

WB

Unknown

Western blot - Anti-NUP50 antibody [EPR9525] (AB151567)

All lanes:

Western blot - Anti-NUP50 antibody [EPR9525] (ab151567) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 50 kDa

false

Carrier free

Anti-NUP50 antibody [EPR9525] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9525

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoporin 50 also known as NUP50 is a nucleoporin protein that plays an essential role in the nuclear pore complex. With a molecular mass of approximately 50 kDa it is primarily found in the nuclear envelope. NUP50 functions mechanically as part of the assembly and regulation of nuclear transport facilitating the translocation of macromolecules between the nucleus and cytoplasm.

Biological function summary

This protein serves an integral role in nucleocytoplasmic transport functioning as a component of the nuclear pore complex. NUP50 interacts with karyopherins which are transport receptors to regulate the directionality and specificity of molecular trafficking between the nucleus and cytoplasm. It also helps disassemble importin-cargo complexes within the nucleus important for the recycling of importins.

Pathways

NUP50 participates in the nuclear transport pathways that are vital for gene expression and cell cycle regulation. It associates closely with the other nucleoporins and transport protein receptors such as Importin-β within these pathways. NUP50 also contributes to DNA replication and repair pathways by facilitating the import of necessary proteins into the nucleus.

NUP50 is associated with certain cancers and neurodegenerative disorders. Its altered expression can impact the normal functioning of the nuclear transport system leading to these pathological conditions. Dysregulation or mutations of NUP50 can interact with other nucleoporins like NUP98 connecting it to disorders involving abnormal cellular proliferation and differentiation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the nuclear pore complex that has a direct role in nuclear protein import (PubMed : 20016008). Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha : beta-cargo complex and importin recycling (PubMed : 20016008). Interacts with regulatory proteins of cell cycle progression including CDKN1B (By similarity). This interaction is required for correct intracellular transport and degradation of CDKN1B (By similarity).

See full target information NUP50

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Annals of the rheumatic diseases 82:1181-1190 PubMed37147113

2023

Autoantibodies identify primary Sjögren's syndrome in patients lacking serum IgG specific for Ro/SS-A and La/SS-B.

Applications

Unspecified application

Species

Unspecified reactive species

Sherri Longobardi,Charmaine Lopez-Davis,Bhuwan Khatri,Constantin Georgescu,Cherilyn Pritchett-Frazee,Christina Lawrence,Astrid Rasmussen,Lida Radfar,Robert Hal Scofield,Alan N Baer,Susan A Robinson,Erika Darrah,Robert C Axtell,Gabriel Pardo,Jonathan D Wren,Kristi A Koelsch,Joel M Guthridge,Judith A James,Christopher J Lessard,Amy Darise Farris

Frontiers in molecular biosciences 9:902220 PubMed35669562

2022

SMARCC1 Enters the Nucleus via KPNA2 and Plays an Oncogenic Role in Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengmao Wei,Jinming Xu,Weiqing Li,Longhua Ou,Yingchen Zhou,Yan Wang,Bentao Shi

Journal of cell science 134: PubMed34409458

2021

Nucleoporin 50 mediates Kcna4 transcription to regulate cardiac electrical activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xueting Gao,Shuai Yu,Yi Guan,Yunli Shen,Liang Xu

Journal of neuroscience research 95:1745-1759 PubMed28029704

2016

Dynamic mislocalizations of nuclear pore complex proteins after focal cerebral ischemia in rat.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Li,Yasuyuki Ohta,Toru Yamashita,Jingwei Shang,Kentaro Deguchi,Tian Feng,Kota Sato,Nozomi Hishikawa,Yumiko Nakano,Koji Abe

Genes & development 29:1224-38 PubMed26080816

2015

The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing.

Applications

Unspecified application

Species

Unspecified reactive species

Filipe V Jacinto,Chris Benner,Martin W Hetzer

Molecular biology of the cell 25:2472-84 PubMed24943837

2014

Nup50 is required for cell differentiation and exhibits transcription-dependent dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Abigail L Buchwalter,Yun Liang,Martin W Hetzer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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