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AB137092

Anti-NUP50 antibody [EPR9526]

4

(1 Review)

|

(6 Publications)

Rabbit Recombinant Monoclonal NUP50 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

NPAP60L, PRO1146, NUP50, Nuclear pore complex protein Nup50, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, Nucleoporin Nup50

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (AB137092)
  • ICC/IF

AbReview32462****

Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (AB137092)

ab137092 (1/200) staining NUP50 in HeLa Cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

This image is courtesy of an abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (AB137092)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (AB137092)

Immunofluorescent analysis of HeLa cells labelling NUP50 with ab137092 at 1/250 dilution.

Western blot - Anti-NUP50 antibody [EPR9526] (AB137092)
  • WB

Unknown

Western blot - Anti-NUP50 antibody [EPR9526] (AB137092)

All lanes:

Western blot - Anti-NUP50 antibody [EPR9526] (ab137092) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9526

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoporin 50 also known as NUP50 is a nucleoporin protein that plays an essential role in the nuclear pore complex. With a molecular mass of approximately 50 kDa it is primarily found in the nuclear envelope. NUP50 functions mechanically as part of the assembly and regulation of nuclear transport facilitating the translocation of macromolecules between the nucleus and cytoplasm.
Biological function summary

This protein serves an integral role in nucleocytoplasmic transport functioning as a component of the nuclear pore complex. NUP50 interacts with karyopherins which are transport receptors to regulate the directionality and specificity of molecular trafficking between the nucleus and cytoplasm. It also helps disassemble importin-cargo complexes within the nucleus important for the recycling of importins.

Pathways

NUP50 participates in the nuclear transport pathways that are vital for gene expression and cell cycle regulation. It associates closely with the other nucleoporins and transport protein receptors such as Importin-β within these pathways. NUP50 also contributes to DNA replication and repair pathways by facilitating the import of necessary proteins into the nucleus.

NUP50 is associated with certain cancers and neurodegenerative disorders. Its altered expression can impact the normal functioning of the nuclear transport system leading to these pathological conditions. Dysregulation or mutations of NUP50 can interact with other nucleoporins like NUP98 connecting it to disorders involving abnormal cellular proliferation and differentiation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the nuclear pore complex that has a direct role in nuclear protein import (PubMed : 20016008). Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha : beta-cargo complex and importin recycling (PubMed : 20016008). Interacts with regulatory proteins of cell cycle progression including CDKN1B (By similarity). This interaction is required for correct intracellular transport and degradation of CDKN1B (By similarity).
See full target information NUP50
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:342 PubMed36670122

2023

Integrative genetic analysis illuminates ALS heritability and identifies risk genes.

Applications

Unspecified application

Species

Unspecified reactive species

Salim Megat,Natalia Mora,Jason Sanogo,Olga Roman,Alberto Catanese,Najwa Ouali Alami,Axel Freischmidt,Xhuljana Mingaj,Hortense De Calbiac,François Muratet,Sylvie Dirrig-Grosch,Stéphane Dieterle,Nick Van Bakel,Kathrin Müller,Kirsten Sieverding,Jochen Weishaupt,Peter Munch Andersen,Markus Weber,Christoph Neuwirth,Markus Margelisch,Andreas Sommacal,Kristel R Van Eijk,Jan H Veldink,Géraldine Lautrette,Philippe Couratier,Agnès Camuzat,Isabelle Le Ber,Maurizio Grassano,Adriano Chio,Tobias Boeckers,Albert C Ludolph,Francesco Roselli,Deniz Yilmazer-Hanke,Stéphanie Millecamps,Edor Kabashi,Erik Storkebaum,Chantal Sellier,Luc Dupuis

Cell reports 40:111106 PubMed35858577

2022

Nuclear RNA binding regulates TDP-43 nuclear localization and passive nuclear export.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Duan,Benjamin L Zaepfel,Vasilisa Aksenova,Mary Dasso,Jeffrey D Rothstein,Petr Kalab,Lindsey R Hayes

The EMBO journal 40:e108788 PubMed34725842

2021

The nucleoporin Nup50 activates the Ran guanine nucleotide exchange factor RCC1 to promote NPC assembly at the end of mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Holzer,Paola De Magistris,Cathrin Gramminger,Ruchika Sachdev,Adriana Magalska,Allana Schooley,Anja Scheufen,Birgitt Lennartz,Marianna Tatarek-Nossol,Hongqi Lue,Monika I Linder,Ulrike Kutay,Christian Preisinger,Daniel Moreno-Andres,Wolfram Antonin

Neuron 107:1124-1140.e11 PubMed32673563

2020

GC Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa N Coyne,Benjamin L Zaepfel,Lindsey Hayes,Boris Fitchman,Yuval Salzberg,En-Ching Luo,Kelly Bowen,Hannah Trost,Stefan Aigner,Frank Rigo,Gene W Yeo,Amnon Harel,Clive N Svendsen,Dhruv Sareen,Jeffrey D Rothstein

Cell 173:958-971.e17 PubMed29628143

2018

Stress Granule Assembly Disrupts Nucleocytoplasmic Transport.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Zhang,J Gavin Daigle,Kathleen M Cunningham,Alyssa N Coyne,Kai Ruan,Jonathan C Grima,Kelly E Bowen,Harsh Wadhwa,Peiguo Yang,Frank Rigo,J Paul Taylor,Aaron D Gitler,Jeffrey D Rothstein,Thomas E Lloyd

Developmental cell 43:141-156.e7 PubMed29065306

2017

Mitotic Disassembly of Nuclear Pore Complexes Involves CDK1- and PLK1-Mediated Phosphorylation of Key Interconnecting Nucleoporins.

Applications

Unspecified application

Species

Unspecified reactive species

Monika I Linder,Mario Köhler,Paul Boersema,Marion Weberruss,Cornelia Wandke,Joseph Marino,Caroline Ashiono,Paola Picotti,Wolfram Antonin,Ulrike Kutay
View all publications
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Product promise

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